Browse > Article
http://dx.doi.org/10.11002/kjfp.2016.23.3.378

Protective effects of quality certified traditional Doenjang in Korea on TNF-α-induced vascular inflammation in human umbilical vein endothelial cells  

Kim, Eun-Ju (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
Jang, Yeon-Jeong (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
Kim, So-Young (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
Choi, Hye-Sun (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
Park, Shin-Young (Fermentated Food Science Division, National Institute of Agricultural Sciences, RDA)
Publication Information
Food Science and Preservation / v.23, no.3, 2016 , pp. 378-386 More about this Journal
Abstract
Anti-atherogenic effects in tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-stimulated human umbilical vein endothelial cells (HUVEC) are involved in the suppression of oxidative stress, cell adhesion molecules, and pro-inflammatory factors. This study investigated the vascular inflammation inhibitory activity of traditional Doenjang plays a key role in the pathogenesis and progression of atherosclerosis. The protective effects of Korean Deonjang was investigated on the expression of cell adhesion molecules (CAMs) in tumor necrosis factor (TNF)-${\alpha}$-induced human umbilical vascular endothelial cells (HUVECs). Deonjang extracts (20, 50, $100{\mu}g/mL$) decreased the expression of 20 ng/mL TNF-${\alpha}$-induced vascular cell adhesion molecule (VCAM)-1 intracellular adhesion molecule (ICAM)-1 proteins, and their corresponding mRNA levels. Nitric oxides (NO) produced by endothlial nitric oxides synthase (eNOS) dilated blood vessels, which had protective effects against platelet and leukocyte adhesion. While TNF-${\alpha}$-induced suppressed the production of nitric oxide in HUVECs, Doenjang restored NO production in HUVECs. In addition, Deonjang reduced the TNF-${\alpha}$-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA levels. These results suggested that Doenjang can inhibited the production of cell adhesion molecules and inflammatory mediators, which could be a potential candidate for preventing atherosclerosis.
Keywords
Doenjang; HUVECs; tumor necrosis factor (TNF)-${\alpha}$; inflammation;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Choi UK, Ji WD, Chung HC, Choi DH, Chung YG (1997) Optimum condition for pigment production and antioxidative activity of the products by Bacillus subtilis DC-2 with response surface methodology. J Korean Soc Food Sci Nutr, 26, 620-624
2 Ross R (1995) Cell biology of atherosclerosis. Annu Rev Physiol, 57, 791-804   DOI
3 Lee ES, Ju HK, Moon TC, Lee EK, Jahng YD, Lee SH, Son JK, Baek SH, Chang HW (2004) Inhibition of nitric oxide and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production by propenone compound through blockade of nuclear factor (NF)-${\kappa}$B activation in cultured murine macrophages. Biol Pharm Bull, 27, 617-620   DOI
4 Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim JY (2007) Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-${\kappa}$B transactivation in LPS-activated RAW 264.7 cells. Mol Cells, 23, 398-404
5 Nathan C (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J, 6, 3051-3064   DOI
6 Mohamed AK, Bierhaus A, Schiekofer S, Tritschler H, Ziegler R, Nawroth PP (1999) The role of oxidative stress and NF-${\kappa}$B activation in late diabetic complications. Biofactors, 10, 157-167   DOI
7 Chen JW, Chen YH, Lin FY, Chen YL, Lin SJ (2003) Ginkgo biloba extract inhibits tumor necrosis factor-${\alpha}$ -induced reactive oxygen species generation, transcription factor activation, and cell adhesion molecule expression in human aortic endothelial cells. Arterioscler Thromb Vasc Biol, 23, 1559-1566   DOI
8 Kanters E, Pasparakis M, Gijbels MJ, Vergouwe MN, Partouns-Hendriks I, Fijneman RJ, Clausen BE, Forster I, Kockx MM, Rajewsky K, Kraal G, Hofker MH, de Winther MP (2003) Inhibition of NF-${\kappa}$B activation in macrophages increases atherosclerosis in LDL receptor-deficient mice. J Clin Invest, 112, 1176-1185   DOI
9 Kunsch C, Luchoomun J, Grey JY, Olliff LK, Saint LB, Arrendale RF, Wasserman MA, Saxena U, Medford RM (2004) Selective inhibition of endothelial and monocyte redox-sensitive genes by AGI-1067: a novel antioxidant and anti-inflammatory agent. J Pharmacol Exp Ther, 308, 820-829
10 Huh JI, Jin SY (2011) A survey on the perception of agricultural food accreditation and traditional food quality certification. J Korean Soc Food Cult, 26, 220-229
11 National Agricultural Products Quality Management Service (2012) Traditional Food Standards. Anyang, Korea, p 88-93, p 99-100
12 Kang JE, Choi HS, Choi HS, Park SY, Song J, Choi JH, Yeo SH, Jung ST (2013) The quality characteristics of commercial Deonjang certified for traditional foods. Korean J Community Living Sci, 24, 537-542   DOI
13 Je JY, Park PJ, Kim EK, Ahn CB (2009) Antioxidant and angiotensin I converting enzyme inhibitory activity of Bambusae caulis in liquamen. Food Chem, 113, 932-935   DOI
14 Lee SJ, Kim EK, Kim YS, Hwang JW, Lee KH, Choi DK, Kang H, Moon SH, Jeon BT, Park PJ (2012) Purification and characterization of a nitric oxide inhibitory peptide from Ruditapes philippinarum. Food Chem Toxicol, 50, 1660-1666   DOI
15 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-254   DOI
16 Vodovotz Y, Russell D, Xie QW, Bogdan C, Nathan C (1995) Vesicle membrane association of nitric oxide synthase in primary mouse macrophage. J Immunol, 154, 2914-2925
17 Kim JY, Jung KS, Jeong HG (2004) Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett, 569, 321-326   DOI
18 Li H, Forstermann U (2000) Nitric oxide in the pathogenesis of vascular disease. J Pathol, 190, 244-254   DOI
19 Sarkar D, Fisher PB (2006) Molecular mechanisms of aging-associated inflammation. Cancer Lett, 236, 13-23   DOI
20 Sung MS, Kim YH, Choi YM, Ham HM, Jeong HS, Lee JS (2011) Anti-inflammatory effect of Erigeron annuus L. flower extract through heme oxygenase-1 induction in RAW 264.7 macrophages. J Korean Soc Food Sci Nutr, 40, 1507-1511   DOI
21 Yoon WJ, Lee JA, Kim KN, Kim JY, Park SY (2007) In vitro anti-inflammatory activity of the Artemisia fukudo extracts in murine macrophage RAW 264.7 cells. Korean J Food Sci Technol, 39, 464-469
22 Hwang SJ, Ballantyne CM, Sharrett AR, Smith LC, Davis CE, Gotto AM Jr, Boerwinkle E (1997) Circulating adhesion molecules VCAM-1, ICAM-1, and E-selectin in carotid atherosclerosis and incident coronary heart disease cases. Circulation, 96, 4219-4225   DOI
23 Li H, Cybulsky MI, Gimbrone MA Jr, Libby P (1993) An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium. Arterioscler Thromb, 13, 197-204   DOI
24 Schmidt A, Goepfert C, Feitsma K, Buddecke E (2002) Lovastatin-stimulated superinduction of E-selectin, ICAM-1 and VCAM-1 in TNF-${\alpha}$ activated human vascular endothelial cells. Atherosclerosis, 164, 57-64   DOI
25 Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E (2007) Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-${\kappa}$B activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-${\kappa}$B activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm, 2007, 45673
26 Rimbach G, Valacchi G, Canali R, Virgili F (2000) Macrophages stimulated with IFN-${\gamma}$ activate NF-${\kappa}B$ and induce MCP-1 gene expression in primary human endothelial cells. Mol Cell Biol Res Commun, 3, 238-242   DOI
27 Lim SY, Rhee SH, Park KY (2004) Inhibitory effect of methanol extract of Doenjang on growth and DNA synthesis of human cancer cells. J Korean Soc Food Sci Nutr, 33, 936-940   DOI
28 Lim SY, Kim KH, Park KY, Lee SH (2007) Effect of solvent fractions from Doenjang on antimutagenicity, growth of tumor cells and production of interleukin-2. J Life Sci, 17, 791-797   DOI
29 Kim MJ, Rhee HS (1990) Studies on the changes of taste compounds during soy paste fermentation. Korean J Food Cook Sci, 6, 1-8
30 Coward L, Barnes NC, Setchell KDR, Barnes S (1993) Genistein, daidzein, and their ${\beta}$-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets. J Agric Food Chem, 41, 1961-1967   DOI
31 Ungar Y, Osundahunsi OF, Shimoni E (2003) Thermal stability of genistein and daidzein and its effect on their antioxidant activity. J Agric Food Chem, 51, 4394-4399   DOI
32 Guo JM, Xiao BX, Liu DH, Grant M, Zhang S, Lai YF, Guo YB, Liu Q (2004) Biphasic effect of daidzein on cell growth of human colon cancer cells. Food Chem Toxicol, 42, 1641-1646   DOI
33 Lee BK, Jang YS, Yi YS, Chung KS, Choi SY (1997) Immunomodulators extracted from Korean-style fermented soybean paste and their function. Isolation of B cell mitogen from Korean-style fermented soybean paste. Korean J Immonol, 19, 559-569
34 Park KY, Moon SH, Baik HS, Cheigh HS (1990) Antimutagenic effect of Doejang (Korean fermented soy paste) toward aflatoxin. J Korean Soc Food Sci Nutr, 19, 158-162
35 Cui CB, Lee EY, Lee DS, Ham SS (2002) Antimutageniic and anticancer effects of ethanol extract from Korean traditional Doenjang added sea tangle. J Korean Soc Food Sci Nutr, 31, 322-328   DOI
36 Kim SH (1998) New trends of studying on potential activities of Doenjang-fibrinolytic activity. Korea Soybean Digest, 15, 8-15
37 Suh HJ, Suh DB, Chung SH, Whang JH, Sung HJ, Yang HC (1994) Purification of ACE inhibitor from soybean paste. Appl Biol Chem, 37, 441-446
38 Hwang JH (1997) Angiotensin I converting enzyme inhibitory effect of Doenjang fermented by B. subtilis SCB-3 isolated from Meju, Korean traditional food. J Koeran Soc Food Sci Nutr, 26, 775-783
39 Yang BK, Jeong SC, Hur NJ, Ha SO, Kim KY, Kym KH, Yun JW, Song CH (2000) Hypoglycemic effect of extracts of soybean paste containing mycelia of mushrooms in streptozotocin-induced diabetic rats. Korean J Mycol, 28, 126-129
40 Choe GS, Lim SY, Choi JS (1998) Antioxidant and nitrite scavenging effect of soybean, Meju and Doenjang. Korean J Life Sci, 8, 473-478
41 Kim MH, Im SS, Kim SH, Kim GE, Lee JH (1994) Antioxidative materials in domestic Meju and Doenjang 2. Separation of lipophilic brown pigment and their antioxidative activity. J Korean Soc Food Nutr, 23, 251-260