Browse > Article
http://dx.doi.org/10.11002/kjfp.2015.22.5.744

Effect of hot water and ethanol extracts from Nelumbo nucifera Gaertner flower on lipid accumulation and reactive oxygen species (ROS) production in adipogenesis of 3T3-L1 cells  

Oh, Ji-Won (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Kye-Hoon (Department of Food Science and Technology, Kongju National University)
Kim, Hey-Ran (Department of Food Science and Technology, Kongju National University)
Lee, Hyo-Ku (Department of Food Science and Technology, Kongju National University)
Publication Information
Food Science and Preservation / v.22, no.5, 2015 , pp. 744-750 More about this Journal
Abstract
The present study was designed to investigate the effects of hot water and ethanol extracts of Nelumbo nucifera Gaertner flower on lipid accumulation and reactive oxygen species (ROS) production during adipogenesis in 3T3-L1 cells. 3T3-L1 preadipocytes were treated with both hot water and ethanol extracts for up to 8 days following standard induction of differentiation. Regarding anti-adipogenic activity, compared with the control, the hot water and ethanol extracts significantly inhibited lipid accumulation (37.4 and 66.6%, respectively) and ROS production (46.4 and 46.8%, respectively) during adipogenesis in 3T3-L1 cells. Treatment with hot water and ethanol extracts significantly inhibited mRNA expression of peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and CCAAT/enhancer-binding protein alpha ($C/EBP{\alpha}$), thereby reducing the mRNA expression of adipocyte-specific fatty acid binding protein (aP2). Moreover, both the extracts significantly inhibited mRNA expression of NADPH oxidase (NOX4). Overall, our research suggests that N. nucifera Gaertner flower extracts might be a valuable source of bioactive compounds that exhibit anti-adipogenic activity and could have applications in the field of medicine and food industry.
Keywords
Nelumbo nucifera Gaertner; extracts; 3T3-L1 cell; lipid accumulation; reactive oxygen species;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Kim MS, Park YJ, Sohn YW (2010) Antioxidative effect and melanogenesis of Nelumbo nucifera stamen extract on cultured Human skin melanoma cells injured by hydrogen peroxide. Korean J Plant Res, 23, 145-150
2 Kwak WS, Lee SK, Lee KJ, Kim KH, Kim HR, Lee HK, Oh JW, Lee OH (2014) Nutritional components and antioxidant activities of Nelumbo nucifera Gaertner flower and its wine. Korean J Food Preserv, 21, 373-380   DOI
3 Yoo JS (2014) Antioxidant and antiobesity effects of lotus (Nelumbo nucifera) root and seed grown in Korea. PhD Thesis. Inha University, Incheon, Korea, p 5-11
4 Seo YM, Park YJ, Choi YS (2009) Study on the protective effect of Nelumbo nucifera GAERTN extract on cultured cerebral neuroglial cells damaged by hexavalent chromium. Flower Res J, 17, 242-245
5 Oh YL (2010) Effect of flower extract on antioxidative of cultured C6 glioma cells damaged by glucose oxidase of reactive oxygen species in Nelumbo nucifera. J Korean Soc People Plants Environ, 13, 17-24
6 Chang MS, Ko EB, Lee HJ, Kim JS, Kim JS, Jee SW, Kim HY, Yeom MH, Kim DH, Kim HK, Park SK (2011) The effects of Nelumbo nucifera on utraviolet-B irradiated human keratinocytes. Korean J Herbol, 26, 45-49
7 Lee KS, Kim MG, Lee KY (2006) Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf. J Korean Soc Food Sci Nutr, 35, 182-186   DOI
8 Park SY, Hwang TY, Kim JH, Moon KD (2001) Quality changes of minimally processed lotus root (Nelumbo nucifera) with browning inhibitors. Korean J Postharvest Sci Technol, 8, 164-168
9 Peng Q, Wei Z, Lau BH (1998) Fructus corni enhances endotherial cell antioxidant defenses. Gen Pharmacol, 31, 221-225   DOI
10 Spiegelman BM, Flier JS (2001) Obesity and the regulation of energy balance. Cell, 104, 531-543   DOI
11 Marcelin G, Chua S (2010) Contributions of adipocyte lipid metabolism to body fat content and implications for the treatment of obesity. Curr Opin Pharmacol, 10, 588-593   DOI
12 Holst D, Grimaldi PA (2002) New factors in the regulation of adipose differentiation and metabolism. Curr Opin Lipidol, 13, 241-245   DOI
13 de Ferranti S, Mozaffarian D (2008) The perfect storm : obesity, adipocyte dysfunction, and metabolic consequences. Clin Chem, 54, 945-955   DOI
14 Attie AD, Scherer PE (2009) Adipocyte metabolism and obesity. J Lipid Res, 50, 395-359   DOI
15 Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol, 7, 885-896   DOI
16 Kim KH (2009) Perspective in regulation of adipogenesis by bioactive food components. Food Sci Ind, 42, 51-57
17 Lee OH, Kwon YI, Hong HD, Park CS, Lee BY, Kim YC (2009) Production of reactive oxygen species and changes in antioxidant enzyme activites during differentiation of 3T3-L1 adipocyte. J Korean Soc Appl Biol Chem, 52, 70-75
18 Valko M, Moncol DJ, Cronin TD, Mazura M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol, 39, 44-84   DOI
19 Lee JY, Yu MR, An BJ (2010) Comparison of biological activity between Nelumbo nucifera G. extracts and comsmetics adding Nelumbo nucifera G.. J Life Sci, 20, 1241-1248   DOI
20 Rosen ED (2005) The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acid, 73, 31-34   DOI
21 Chance B, Oshino N (1971) Kinetics and mechanism of catalase in peroxisomes of the mitochondrial fraction. Biochem J, 112, 225-233
22 Chang MS, Kim HM, Yang WM, Kim DR, Park EH, Ko EB, Choi MJ, Kim HY, Oh JH, Shim KJ, Yoon JW, Park SK (2007) Inhibitory effects of Nelumbo nucifera on tyrosinase activity and melanogenesis in Clone M-3 melanocyte cells. Korean J Herbol, 22, 87-94
23 Evans RM, Barish GD, Wang YX (2004) PPARs and the complex journey to obesity. Nat Med, 10, 355-361   DOI
24 Pablo C, Daiana S, Soledad C, Virginia D, Lelia D, Juan CC, Liliana NG (2011) N-Acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes. Int J Mol Sci, 12, 6936-6951   DOI
25 Sampson N, Koziel R, Zenzmaier C, Bubendorf L, Plas E, Jansen-Durr P, Berger P (2011) ROS signaling by NOX4 drives fibroblast-to-myofibroblast differentiation in the diseased prostatic stroma. Mol Endocrinol, 25, 503-515   DOI
26 Basuroy S, Tcheranova D, Bhattacharya S, Leffler CW, Parfenova H (2011) Nox4 NADPH oxidase-derived reactive oxygen species, via endogenous carbon monoxide, promote survival of brain endothelial cells during TNF-${\alpha}$-induced apoptosis. Am J Physiol Cell Physiol, 300, C256-265   DOI
27 Yang RZ, Wei XL, Gao FF, Wang LS, Zhang HJ, Xu YJ, Li CH, Ge YX, Zhang JJ, Zhang J (2009) Simultaneous analysis of anthocyanins and flavonols in petals of lotus (Nelumbo) cultivars by high-performance liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry. J Chromatogr A, 1216, 106-112   DOI
28 Lee JJ, Park SY, Lee MY (2006) Effect of lotus root (Nelumbo nucifera G.) on lipid metabolism in rats with diet-induced hypercholesterolemia. Korean J Food Preserv, 13, 634-642
29 Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I (2004) Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest, 114, 1752-1761   DOI