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http://dx.doi.org/10.11002/kjfp.2015.22.4.559

Survey and method validation of simultaneous quantitative analysis of T-2 and HT-2 toxins in cereals  

Paek, Ockjin (Department of Chemistry, Sang Myung University)
Kang, Teabeom (Department of Chemistry, Sang Myung University)
Publication Information
Food Science and Preservation / v.22, no.4, 2015 , pp. 559-566 More about this Journal
Abstract
The aim of this study was to develop an analytical method for determination of T-2 toxin and HT-2 toxin level in cereals and to survey their levels using LC-MS/MS. The T-2 and HT-2 toxins were simultaneously analyzed by electrospray ionization with a positive ion mode and multiple reaction monitoring (MRM) after filteration and immuno-affinity column clean-up. A matrix-matched standard calibration used for quantification and recoveries of T-2 and HT-3 toxins were in the range of $100.6{\pm}7.2%$ and $96.8{\pm}9.4%$, respectively. Limits of detection and quantification of T-2 and HT-2 toxins were estimated to be 0.5 and $1.5{\mu}g/kg$, respectively. Each repeatability (RSRr) of T-2 and HT-2 toxins was determined to be 0.9~6.0%, and 4.9~6.1%, respectively. Total 115 samples cereals were collected from 9 types of cereals for analysis. The positive percentages of T-2 and HT-2 toxins obtained from collected samples were found to be 72% and 80%, respectively. The contamination level of T-2 toxin and HT-2 toxin in cereals were $37.1{\mu}g/kg$, and $5.4{\mu}g/kg$, respectively. Therefore, this study suggests that the developed method could be an useful analytical method to determine the T-2 and HT-2 toxin level in cereals and the present data could be used as a reference to estimate the risk assessment.
Keywords
T-2 toxin; HT-2 toxin; LC-MS/MS; cereals; survey;
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Times Cited By KSCI : 2  (Citation Analysis)
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