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http://dx.doi.org/10.11002/kjfp.2014.21.5.740

Anti-inflammatory effect of Polygonum multiflorum extraction in activated RAW 264.7 cells with lipopolysaccharide  

Lee, Eunsu (Department of Cosmeceutical Science, Daegu Hanny University)
Kim, Hyeongjeong (Department of Cosmeceutical Science, Daegu Hanny University)
Yu, Jae-Myo (Department of Cosmeceutical Science, Daegu Hanny University)
Cho, Yong-Hun (Department of Cosmeceutical Science, Daegu Hanny University)
Kim, Dong-In (Department of Cosmeceutical Science, Daegu Hanny University)
Shin, Yuhyeon (Department of Cosmeceutical Science, Daegu Hanny University)
Cho, Yeongje (School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University)
Kwon, O-Jun (Regional industry Evaluation Agency for Gyeongbuk)
An, Bongjeon (Department of Cosmeceutical Science, Daegu Hanny University)
Publication Information
Food Science and Preservation / v.21, no.5, 2014 , pp. 740-746 More about this Journal
Abstract
The anti-inflammatory effects of Polygonum multiflorum water extracts (PMWs) and Polygonum multiflorum 70 % ethanol extracts(PMEs) were investigated using lipopolysaccharide-induce by inflammatory response. The inhibitory effects of PMWs and PMEs on the production of nitric oxide (NO) and pro - inflammatory cytokines in LPS - activated Raw 264.7 cells were investigated. The effects were examined after reducing production of Nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels. RAW 264.7 cells were cultured with LPS ($1{\mu}g/mL$) in the presence or absence of PMWs and PMEs for 24 h to determine their NO, iNOS, COX-2 levels. During the entire experimental period 10, 25, 50 and $100{\mu}g/mL$ of PMWs and PMEs showed no cytotoxicity. At these concentrations, PMWs and PMEs concentration dependently reduced the production of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-$1{\beta}$ (IL-$1{\beta}$). PMWs and PMEs were inhibited the activittion of iNOS, COX-2 by 89%, 54%, 91% and 57% respectively, at $100{\mu}g/mL$. These results indicate that PMWs and PMEs significantly reduces the effect of oxidative and inflammatory cytokines.
Keywords
anti-inflammatory; COX-2; cytokine; iNOS; Polygonum multiflorum;
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Times Cited By KSCI : 4  (Citation Analysis)
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