Browse > Article
http://dx.doi.org/10.11002/kjfp.2013.20.3.419

Bactericidal effect of 461 nm blue light emitting diode on pathogenic bacteria  

Do, Jung Sun (LED-IT Fusion Technology Research Center, Yeungnam University)
Bang, Woo Suk (LED-IT Fusion Technology Research Center, Yeungnam University)
Publication Information
Food Science and Preservation / v.20, no.3, 2013 , pp. 419-423 More about this Journal
Abstract
The objective of this study was to characterize the bactericidal effect of 461nm visible-light LED on three common foodborne bacteria: Escherichia coli O157:H7, Staphylococcus aureus and Vibrio parahaemolyticus. Tests were conducted against pathogen strains that were treated with 461nm LED for 10 h at $15^{\circ}C$. The E. coli (ATCC 43894, ATCC 8739 and ATCC 35150) and the S. aureus (ATCC 27664, ATCC 19095 and ATCC 43300) had average reductions of 2.5, 6.6, 1.5, 2.5 and 2.0 log CFU/mL, respectively, after they were exposed for 10 h to 461nm LED light (p<0.05). In contrast, V. parahaemolyticus (ATCC 43969) had 6 log CFU/mL reductions after it was exposed for 4 h to 461nm LED light. The results showed that both the Gram-positive and Gram-negative bacteria were inactivated with 461nm LED light exposure. Also, the Gram-negative bacteria were more sensitive to the LED treatment than the Gram-positive bacteria. These results show the potential use of 461nm LED as a food preservation and application technology.
Keywords
461 nm LED; blue light; microorganism; photosensitization;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 BIR research group (2010) Eco-friendly, high-efficient LED technology development trends and market outlook. BIR Inc, Seoul, Korea p 27-188
2 United States Environmental Protection Agency (2003) Ultraviolet disinfection guidance manual. NSCEP, EPA 815-D-03-007
3 Young AR (2006) Acute effects of UVR on human eyes and skin. Prog Biophys Mol Biol, 92, 80-85   DOI   ScienceOn
4 Halliday GM, Norval M, Byrne SN, Huang XX, Wolf P (2008) The effects of sunlight on the skin. Drug discovery today: Disease Mechanisms, 5, 201-209   DOI   ScienceOn
5 Ikehata H, Ono T (2011) The mechanisms of UV mutagenesis. J Radiat Res, 52, 115-125   DOI   ScienceOn
6 Maisch T (2007) Anti-microbial photodynamic therapy: useful in the future? Lasers Med Sci, 22, 83-91   DOI   ScienceOn
7 Endarko E, Maclean M, Timoshkin IV, Macgregor SJ, Anderson JG (2012) High-Intensity 405 nm Light Inactivation of Listeria monocytogenes. J photochem photobiol, 88, 1280-1286   DOI   ScienceOn
8 Luksiene Z (2005) New approach to inactivation of harmful and pathogenic microorganisms by Photosensitization. Food Technol Biotechnol, 43, 411-418
9 Luksiene Z, Zukauskas A (2009) Prospects of photosensitization in control of pathogenic and harmful micro-organisms. J Appl Microbiol, 107, 1415-1424   DOI   ScienceOn
10 Buchovec I, Paskeviciute E, Luksiene Z (2010) Photosensitization-based inactivation of food pathogen Listeria monocytogenes in vitro and on the surface of packaging material. J Photochem Photobiol B, 99, 9-14   DOI   ScienceOn
11 Barolet D (2008) Light-emitting diodes (LEDs) in dermatology. Semin Cutan Med Surg, 27, 227-238   DOI   ScienceOn
12 Baek KH, Jang MH, Kwack YB, Lee SW, Yun HK (2010) Regulation of acid contents in kiwifruit irradiated by various wavelength of light emitting diode during postharvest storage. Clean Tech, 16, 88-94
13 Oh MS, Lee HS (2010) Development of phototactic test apparatus equipped with light source for monitoring Pests. J Appl Biol Chem, 53, 248-252   DOI   ScienceOn
14 Oh SJ, Park DS, Yang HS, Yoon YH, Honjo T (2007) Bioremediation on the benrhic layer in polluted inner bay by promotion of microphytobenthos growth using Light Emitting Diode (LED). J Kor Soc MEE, 10, 93-101
15 Durantini EN (2006) Photodynamic inactivation of bacteria. Curr Bioactive Compounds, 2, 127-142   DOI
16 Kim SW (2011) In vitro bactericidal effect of red, green and blue (RGB) light emitting diode (LED) irradiation. MS thesis Chonnam National University Gwangju, Korea
17 Murdoch LE, Maclean M, Endarko E, MacGregor SJ, Anderson JG (2012) Bactericidal effects of 405 nm light exposure demonstrated by inactivation of Escherichia, Salmonella, Shigella, Listeria, and Mycobacterium species in liquid suspensions and on exposed surfaces. The Scientific World Journal, 137805, 1-8
18 Maclean M, MacGregor SJ, Andersom JG, and Woolsey G (2009) Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array. Appl Environ Microbiol, 75, 1932-1937   DOI   ScienceOn
19 Maclean M, Macgregor SJ, Anderson JG, Woolsey (2008) The role of oxygen in the visible-light inactivation of Staphylococcus aureus. J Photochem Photobiol, 92, 180-184   DOI   ScienceOn
20 Fotinos N, Convert M, Piffaretti JC, Gurny R, Lange N (2008) Effects on gram-negative and gram-positive bacteria mediated by 5-aminolevulinic Acid and 5-aminolevulinic acid derivatives. Antimicrob Agents Chemother, 52, 1366-1373   DOI   ScienceOn
21 Um GY (2008) display engineering. gijeon, Seoul, Korea p 320-368
22 Nitzan Y, Salmon DM, Shporen E, Malik Z (2004) ALA induced photodynamic effects on Gram positive and negative bacteria. Photochem Photobiol Sci, 3, 430-435   DOI   ScienceOn
23 Korea Food and Drug Administration (2012) Available from: http://www.mfds.go.kr/e-stat/index.do Accessed on 2012
24 Kim YS, Shin DH (2003) Researches on the volatile antimicrobial compounds from edible plants and their food application. Korean J Food Sci Technol, 35, 159-165
25 Kim YS, Park IS, Ha SD (2009) Application sanitizer for the control of microorganisms in Food. Food Sci Indus, 42, 26-35
26 Shin JK, Kim BR, Kim AJ (2010) Nonthermal food processing technology using electric Power. Food Sci Indus, 43, 21-34
27 Nam SY, Park MS, Gang JG (2010) The LED technology and application of green energy. SangHakdang, Seoul, Korea p 11-114