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http://dx.doi.org/10.11002/kjfp.2012.19.4.577

Establishment of Extraction Conditions for the Optimization of the Black Garlic Antioxidant Activity Using the Response Surface Methodology  

Kang, Jae-Ran (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences Gyeongsang National University)
Lee, Soo-Jung (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences Gyeongsang National University)
Kwon, Hyo-Jin (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences Gyeongsang National University)
Kwon, Min-Hye (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences Gyeongsang National University)
Sung, Nak-Ju (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences Gyeongsang National University)
Publication Information
Food Science and Preservation / v.19, no.4, 2012 , pp. 577-585 More about this Journal
Abstract
This study was conducted to establish the extraction conditions for the optimization of the biological activities of black garlic using the response surface methodology (RSM). The extraction conditions were based on the central composite design, with 15 kinds of variations in the extraction temperature (50-$90^{\circ}C$ ), extraction time (3-15 hrs), and ethanol concentration (0-100%). The total phenol, flavonoids, thiosulfinate contents, and anti-oxidant activity of black garlic extract were significantly higher at the J condition ($90^{\circ}C$ for 9 hrs with a 50% ethanol concentration). In this condition, the biological activities such as DPPH radical scavenging (66.10%), ABTS radical scavenging activity (75.02%), and reducing power by of FRAP (375.4 ${\mu}M/mL$) were excellent. Overall, the extraction conditions for the optimized biological activities of black garlic via RSM were expected to be at $89.68^{\circ}C$ for 9.79 hrs with a 55.72% ethanol concentration. The actual values were 96.4-114.8% of the predicted values.
Keywords
Black garlic; biological activity; response surface methodology;
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Times Cited By KSCI : 19  (Citation Analysis)
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1 Kim HY, Woo KS, Hwang IG, Lee YR, Jeong HS (2008) Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J Food Sci Thechnol, 40, 166-170   과학기술학회마을
2 Choi YM, Kim MH, Shin JJ, Park JM, Lee JS (2003) The antioxidant activities of some commercial test. J Korean Soc Food Sci Nutr, 32, 723-727   DOI
3 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237   DOI   ScienceOn
4 Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem, 239, 70-76   DOI   ScienceOn
5 Jeong JE, Shim SP, Jeong YS, Jung HK, Kim YC, Hong JH (2011) Optimization of extraction conditions for ethanol extracts from Citrus unshiu peel by response surface methodology. Korean J Food Preserv, 18, 755-763   과학기술학회마을   DOI
6 Jo IH, Kim CY, Lee TW, Lee GH, Choi YH (2010) Optimization of extraction of effective components from Vitis coignetiae, the crimson glory vine. Korean J Food Preserv, 17, 659-666   과학기술학회마을
7 Sin HN, Yusof S, Hamid NSA, Rahman RA (2006) Optimization of hot water extraction for sapodilla juice using response surface methodology. J Foods Eng, 74, 352-358   DOI   ScienceOn
8 Joglekar AM, May AT (1987) Product excellence through design of experiments. Cereal Foods World, 32, 857-868
9 Na GM, Han HS, Ye SH, Kim HK (2004) Physiological activity of medicinal plant extracts. Korean J Food Preserv, 11, 388-393   과학기술학회마을
10 Lee JW, Lee SK, Do JH, Yang JW (2000) Determination of total phenolic compounds from Korean red ginseng, and their extraction conditions. J Ginseng Res, 24, 64-67   과학기술학회마을
11 Lee HJ, Do JR, Kwon JH, Kim HK (2010) Antioxidant effects of Viscum album L. extracts by extraction conditions. J Korean Soc Food Sci Nutr, 39, 14-19   과학기술학회마을   DOI
12 Choi YH, Shim YS, Kim CT, Lee C, Shin DB (2007) Characteristics of thiosulfinates and volatile sulfur compounds from blanched garlic reacted with allinase. Korean J Food Sci Technol, 39, 600-607
13 Kwon SK (2003) Organosulfur compounds from Allium sativum and physiological activities. J Appl Pharmacol, 11, 8-32
14 Tsao SM, Yin MC (2001) In vitro activity of garlic oil and four diallyl sulfides against antibiotic-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. J Antimicrob Chemother, 47, 665-670   DOI   ScienceOn
15 Chung JG (1999) Effects of garlic components diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity in human bladder tumor cells. Drug Chem Toxicol, 22, 343-358   DOI   ScienceOn
16 Shin JH, Choi DJ, Chung MJ, Kang MJ, Sung NJ (2008) Changes of physicochemical components and antioxidant activity of aged garlic at different temperatures. J Korean Soc Food Sci Nutr, 37, 1174-1181   과학기술학회마을   DOI
17 Nagae S, Ushijima M, Hatono S, Imai J, Kasuga S, Matsuura H, Itakura Y, Higashi Y (1994) Pharmacokinetics of the garlic compound S-allylcysteine. Planta Med, 60, 214-217   DOI
18 Ichikawa M, Yoshida J, Ide N, Sasaoka T, Yamaguchi H, Ono K (2006) Tetrahydro-$\beta$-carboline derivatives in aged garlic extract show antioxidant properties. J Nutr, 136, 726S-731S   DOI
19 Lee SE, Seong NS, Bang JK (2003) Antioxidative activities of Korean medicinal plants. Korean J Medicinal Crop Sci, 11, 127-134   과학기술학회마을
20 Dewanto V, Wu X, Adom KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem, 50, 3010-3014   DOI   ScienceOn
21 Hwang EY, Kim DH, Kim HJ, Hwang JY, Park TS, Lee IS, Son JH (2011) Antioxidant activities and nitric oxide production of medicine plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora). J Appl Biol Chem, 54, 171-177   과학기술학회마을   DOI
22 Lim TS, Do JR, Kwon OJ, Kim HK (2007) Monitoring on extraction yields and functional properties of onion (Allium cepa) extracts by using response surface methodology. J Korean Soc Food Sci Nutr, 36, 105-110   과학기술학회마을   DOI
23 Shin JH, Choi DJ, Lee SJ, Cha JY, Kim JG, Sung NJ (2008) Change of physicochemical components and antioxidant activity of garlic during its processing. J Life Sci, 18, 1123-1131   DOI
24 Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ (2008) Antioxidant activity of black garlic (Allium sativum L). J Korean Soc Food Sci Nutr, 37, 965-971   과학기술학회마을   DOI
25 Shin JH, Lee HG, Kang MJ, Lee SJ, Sung NJ (2010) Antioxidant activity of solvent fraction from black garlic. J Korean Soc Food Sci Nutr, 39, 933-940   과학기술학회마을   DOI
26 Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ, Shin JH (2008) Physicochemical characteristics of black garlic (Allium sativum L). J Korean Soc Food Sci Nutr, 37, 465-471   과학기술학회마을   DOI
27 Gutfinger T (1981) Polyphenols in olive oil. J Am Oil Chem Soc, 58, 966-968   DOI   ScienceOn
28 You BR, Kim HR, Kim MJ, Kim MR (2011) Comparison of the quality characteristics and antioxidant activities of the commercial black garlic and lab-prepared fermented and aged black garlic. J Korean Soc Food Sci Nutr, 40, 366-371   과학기술학회마을   DOI
29 Cha JY (2009) Functional components and biological activities of marketing black garlic. MS Thesis, Gyeongsang National University, Jinju, Korea
30 Jeon SY, Baek JH, Jeong EJ, Cha YJ (2012) Volatile flavor compounds in commercial black garlic extracts. J Korean Soc Food Sci Nutr, 41, 116-122   과학기술학회마을   DOI
31 Moreno MIN, Isla MI, Sampietro AR, Vattuone MA (2000) Comparison of the free radical scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71, 109-114   DOI   ScienceOn
32 Freeman GG, Mcbreen F (1973) A rapid spectrophotometric methods of determination of thiosulfinate in onion and its significance in flavor studies. Biochem Soc Trans, 1, 1150-1154   DOI
33 Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200   DOI   ScienceOn
34 Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS (2006) Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J Food Sci Technol, 38, 331-336   과학기술학회마을
35 Lee YR, Woo KS, Hwang IG, Kim HY, Lee SH, Lee J, Jeong HS (2012) Physicochemical properties and antioxidant activities of garlic (Allium sativum L.) with different heat and pressure treatments. J Korean Soc Food Sci Nutr, 41, 278-282   과학기술학회마을   DOI   ScienceOn