Browse > Article
http://dx.doi.org/10.11002/kjfp.2012.19.3.438

Isolation of Calcium-Binding Peptides from Barley Protein Hydrolysates  

Lee, Ji-Hye (Department of Food Science and Technology, Chungnam National University)
Choi, Dong-Won (Department of Food Science and Technology, Chungnam National University)
Song, Kyung-Bin (Department of Food Science and Technology, Chungnam National University)
Publication Information
Food Science and Preservation / v.19, no.3, 2012 , pp. 438-442 More about this Journal
Abstract
To prepare calcium-binding peptides as calcium supplement, barley proteins were hydrolyzed using Flavourzyme for 18 h and the hydrolysates were ultra-filtered under 3 kDa as a molecular weight. The resultant filtered peptides were fractionated using ion exchange and normal-phase high performance liquid chromatography. Then each fraction that was obtained was determined for its calcium-binding activity to isolate the calcium-binding peptides. As a result, the highest calcium-binding peptide fraction was obtained, and the results suggest that barley protein hydrolysates can be used as a calcium supplement.
Keywords
calcium-binding peptides; barley protein hydrolysates; purification;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Barclay JW, Morgan A, Burgoyne RD (2005) Calciumdependent regulation of exocytosis. Cell Calcium 38, 343-353   DOI   ScienceOn
2 Jeon SJ, Lee JH, Song KB (2010) Preparation for calcium and iron-binding peptides from rice bran protein hydrolysates. J Appl Biol Chem, 53, 174-178   과학기술학회마을   DOI
3 Lee JH, Jeon SJ, Song KB (2010) Isolation of a calcium-binding fraction from hot-water extract of Smilax rhizoma. Korean J Food Preserv, 17, 903-907   과학기술학회마을
4 Lee SH, Song KB (2009) Isolation of a calcium-binding peptide from enzymatic hydrolysates of porcine blood plasma protein. J Korean Soc Appl Biol Chem, 52,290-294   과학기술학회마을   DOI
5 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685   DOI   ScienceOn
6 Ekund A (1976) On the determination of available lysine in casein and rapeseed protein concentration using 2,4,6-trinitrobenzenesulfonic acid (TNBS) as a reagent of free $\alpha$-amino group of lysine. Anal Chem, 70, 434-439
7 Gitelman HJ (1967) An improved automated procedure for the determination of calcium in biological specimens. Anal Biochem, 18, 521-531   DOI
8 Hara H, Funabiki R, Iwata M, Yamazaki KI (1984) Portal absorption of small peptides in rats under unrestrained conditions. J Nutr, 114, 1122-1129
9 Jung WK, Lee BJ, Kim SK (2006) Fish-bone peptide increases calcium solubility and bioavailability in ovariectomised rats. Brit J Nutr, 95, 124-128   DOI   ScienceOn
10 Korhonen H, Pihlanto A (2006) Bioactive peptides: Production and functionality. Int Dairy J, 16, 945-960   DOI   ScienceOn
11 Miquel E, Alegria A, Barbera R, Farre R (2005) Speciation analysis of calcium, iron, and zinc in casein phosphopeptide fraction from toddler milk-based formula by anion exchange and reversed-phase high-performance liquid chromatography-mass spectrometry/flame atomicabsorption spectroscopy. Anal Bioanal Chem, 381, 1082-1088   DOI   ScienceOn
12 Jeon SJ, Lee JH, Song KB (2010) Isolation of a calcium-binding peptide from chlorella protein hydrolysates. J Food Sci Nutr, 15, 282-286   과학기술학회마을   DOI
13 Choe JS, Youn JY (2005) The chemical composition of barley and wheat varieties. J Korean Soc Food Sci Nutr, 34, 223-229   과학기술학회마을   DOI
14 Jun HI, Cha MN, Song GS, Yoo CS, Kim YT, Kim YS (2011) Physicochemical properties and cooking quality of naked waxy barley (Saechalssal bori). Korean J Food Preserv, 18, 165-170   과학기술학회마을   DOI
15 Park TS, Lee SY, Kim HJ, Kim KT, Kim YJ, Jeong IH, Do WN, Lee HJ (2009) Extracts of adlay, barley and rice bran have antioxidant activity and modulate fatty acid metabolism in adipocytes. Korean J Food Nutr, 22, 456-462   과학기술학회마을
16 McIntosh GH, Whyte J, McArthur R, Nestel PJ (1991) Barley and wheat foods: Influence on plasma cholesterol concentration in hypercholesterolemic men1-3. Am J Clin Nutr, 53, 1205-1209
17 Bowers GN, Brassard C (1986) Measurement of ionized calcium serum with ion-selective electrode: A mature technology that can meet the daily service needs. Clin Chem, 32, 1437-1447
18 Yang EJ, Cho YS, Choi MS, Woo MN, Kim MJ, Shon MY, Lee MK (2009) Effect of young barley leaf on lipid contents and hepatic lipid-regulating enzyme activities in mice fed high-fat diet. Korean J Nutr, 42, 14-22   과학기술학회마을
19 Chang HG, Jung IH (1994) The physicochemical properties and cooking qualities of barley. J Korean Soc Food Nutr, 23, 816-821   과학기술학회마을
20 Titchenal CA, Dobbs J (2007) A system to assess the quality of food sources of calcium. J Food Compos Anal, 20, 717-724   DOI   ScienceOn