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Determination of Optimum Hydrolysis Conditions for Flavonoid Analysis in Plant Leaves  

Park, Jin-Soon (Department of Food Science & Technology, Kyungpook National University)
Hwang, In-Wook (Department of Food Science & Technology, Kyungpook National University)
Zheng, Hu-Zhe (Department of Food Science & Technology, Kyungpook National University)
Kim, Suk-Kyung (Food and Bio-industry Research Institute, Kyungpook National University)
Chung, Shin-Kyo (Department of Food Science & Technology, Kyungpook National University)
Publication Information
Food Science and Preservation / v.17, no.2, 2010 , pp. 261-266 More about this Journal
Abstract
Acidic hydrolysis conditions prior to HPLC analysis of plant flavonoids was investigated by response surface methodology (RSM), using leaves of Ginkgo biloba L. Ten hydrolysis conditions using 0.5-2.5 M HCl and 0.5-2.5 hr hydrolysis time were chosen to form a central composite rotatable design (CCRD), and optimization by RSM was achieved by measuring myricetin, quercetin, and kaempferol levels by HPLC. Optimum hydrolysis condition was 1.5 M HCl for 1.5 hr. The levels of flavonoids obtained under the condition were in good agreement with predicted maximum values, with yields of more than 95%. These optimum hydrolysis condition was applied to analysis of flavonoid content in the leaves of Camellia sinensis, Ficus carica, and Sageretia theezans.
Keywords
flavonoid contents; acid hydrolysis condition; optimization; RSM; Ginkgo biloba L;
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