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Extraction and Bleaching of Acid- and Pepsin-Soluble Collagens from Shark Skin and Muscle  

Kim, Jae-Won (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Kim, Do-Kyun (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Kim, Mee-Jung (Devision of Hotel Culinary Arts and Bakery, Shinsung University)
Kim, Soon-Dong (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Publication Information
Food Science and Preservation / v.17, no.1, 2010 , pp. 91-99 More about this Journal
Abstract
Extraction and bleaching of citric acid- and pepsin-soluble collagens (ASC and PSC, respectively) from shark (Isurus oxyrinchus) skin and muscle were investigated. The optimal sodium hydroxide concentration for extraction was 0.3 M and the optimal treatment time for removal of foreign material was 9 h. The optimal sodium hypochlorite level for bleaching of shark skin was 0.48% (w/v), and sodium hypochlorite was a better bleaching agent than acetone, hydrogen peroxide (10%, v/v), sodium sulfite (0.48%, w/v), sodium thiosulfate (0.48%, w/v), or sodium metabisulfite (0.48%, w/v). Optimal citric acid concentration and extraction time for ASC were 0.3 M and 72 h, respectively, whereas optimal conditions for extraction of PSC were treatment with 0.1 M citric acid containing 0.1% (w/v) pepsin for 24 h. Protein contents in ASSC (acid-soluble shark skin collagen), ASMC (acid-soluble shark meat collagen), PSSC (pepsin-soluble shark skin collagen), and PSMC (pepsin-soluble shark meat collagen) were 88.66%, 83.09%, 90.33%, and 84.81% (on a dry weight basis), respectively, similar to that of commercial marine collagen (88.86%). Net collagen contents of ASSC, ASMC, PSSC, and PSMC, calculated from hydroxyproline levels, were 70.31%, 25.70%, 83.09%, and 32.94%, respectively. The yields of freeze-dried ASSC, ASMC, PSSC,and PSMC were 57.22%, 53.85%, 23.28%, and 20.61%.
Keywords
shark; collagen; extraction; bleaching; acid soluble collagen; pepsin soluble collagen;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Senaratne, L.S., Park, P. and Kim, S. (2006) Isolation and characterization of collagen from brown backed toadfish (Lagocephalus gloveri) skin. Bioresource Technol., 97, 191-197   DOI   ScienceOn
2 Jerome, S.P., Gabrielle, L. and Raul, F. (1998) Identification of collagen fibris in Scleroderma skin. J. Invest. Dermatol., 90, 48-54
3 Kwon, M.C., Kim, C.H., Kim, H.S., Syed, A.Q., Hwang, B.Y. and Lee, H.Y. (2007) Anti-wrinkle activity of low molecular weight peptides derived from collagen isolated from Asterias amurensis. Kor. J. Food Sci. Technol., 39, 625-629
4 Li, H., Liu, B.L., Gao, L.Z. and Chen, H.L. (2004) Studies on bullfrog skin collagen. Food Chem., 84, 65-69   DOI   ScienceOn
5 Richard, M., Judy G.Z. (1980) Type IV collagen from chicken muscular tissues. Isolation and characterization of the pepsin-resistant fragments. Biochmistry, 19, 4065-4072   DOI   ScienceOn
6 Yamaguchi, K., Lavety, J. and Love, R.M. (1976) The connective tissues of fishVIII. Comparative studies on hake, cod and catfish collagen. J. Food Technol., 11, 389-399   DOI
7 ChandraRajan, J. (1978) Separation of typeIII collagen from type I collagen and pepsin by differential denaturation and renaturation. Biochem. Biophys. Res. Comm., 83, 180-186   DOI   ScienceOn
8 A.O.A.C. (2000) Official Methods of Analysis of the AOAC INTERNATIONAL. 17th edition. William Horwitz, ed. Association of Official Analytical Chemists, U.S.A.
9 Woo, J.W., Yu, S.J., Cho, S.M., Lee, Y.B. and Kim, S.B. (2008) Extraction optimization and properties of collagen from yellowfin tuna(Thunnus albacares) dorsal skin. Food Hydrocolloids, 22, 879-887   DOI   ScienceOn
10 Rutala, W.A. and Weber, D.J. (1997) Uses of inorganic hypochlorite (bleach) in health-care facilities. Chinese Microbiol. Rev., 10, 597-610
11 Ahn, C.W., Nam, H.S, Lee, H.J. and Shin, Y.C. (1994) Decolorization of shrimp chitin using sodium hypochlorite. Korean J. Food Sci. Technol., 6, 787-790
12 Hu, Y., Zhao, W., Qian, X. and Zhang, L. (2003) Effects of oral administration of type II collagen on adjuvant arthritis in rats and its mechanisms. Chi. Med. J., 116, 284-287
13 Murphy, M.G., Wright, V., Ackman, R.G. and Horackova, M. (1997) Diets enriched in menhaden fish oil, seal oil, or shark liver oil have distinct effects on the lipid and fatty acid composition of guinea pig heart. Molecular Cell. Biochem., 177, 257-269   DOI
14 Weiss, J.B. and Ayad, S. (1982) An introduction to collagen. In: Collagen in health and disease. Weiss JB, Jayson MIV. (eds.), Ch 1, Churchill Livingstone, NY, pp. 1-17
15 Davison, P.F., Levine, L., Drake, M.P., Ruvin, A.L. and Bump, S. (1967) The serological specificity of tropocollagen telopeptides. J. Exp. Med., 126, 331-345   DOI   ScienceOn
16 Steffen, G., Edward, J. and Miller. (1979) Characterization of lens capsule collagen: Evidence for the presence of two unique chains in molecules derived from major basement membrane structures. Arch. Biochem. Biophys., 198, 370-378   DOI   ScienceOn
17 Bergaman, I. and Loxley, R. (1963) Tow improved and simplified methods for the spectrophotometric determination of hydroxyproline. Anal. Chem., 35, 1961-1963   DOI
18 McCormick, R.J. (1994) Structure and properties of tissues. In: Muscle foods, meat poultry and seafood technology. Kinsman DM, Kotula AW, Breidenstein BC. (eds.), Chapman and Hall, NY, pp. 25-62
19 Taylor, S,L., Higley, N,C. and Bush, R,K. (1986) Sulfites in foods: uses, analytical methods, residues, fate, exposure assessment, metabolism, toxicity, and hypersensitivity. Adv. Food Res., pp. 2-39
20 Raphael, D., Wong, T.A., Hoodnik, R. and Borden, B.G. (1981) The effect of temperature on the bactericidal efficiency of sodium hypochlorite. J. Endocrinol., 7, 330-334
21 Tonnesen, MG., Feng, X. and Clark, R.A. (2000) Angiogenesis in wound healing. J. Invest. Dermatol. Symp. Proc., 5, 40-46
22 Sato, K., Yoshinaka, R., Sato, M. and Shimizu, Y. (1986) Collagen content in the muscle of fishes in association with their swimming movement and meat texture. Nippon Suisan Gakk., 52, 1595-1600   DOI
23 Knapp, T.R., Luck, E. and Daniels, J.R. (1977) Behaviour of solubilised collagen as a bioimplant. J. Surg. Res., 23, 96-105   DOI   ScienceOn
24 Ronald, F. (1995) Effect of sodium hypochlorite exposure on infectivity of cryptosporidium parvum oocysts for neonatal BALB/c Mice. Appl. Environ. Microbiol., 61, 844-846
25 Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.L. (1951) Protein measurement folin phenol reagent. J. Biol. Chem., 193, 265-275
26 Brown, T.T. (1981) Laborotory evaluation of selected disinfectants as virucidal agents against porcine pavovirus, pseudorabies virus, and transmissible gastroenteritis virus. Am. J. Vet. Res. 42, 1033-1036
27 Ahn, C,W., Nam, H,S., Lee, H,J. and Shin, Y,C. (1994) Decolorization of shrimp chitin using sodium hypochlorite. Korean J. Food Sci., 26, 787-790
28 Kim, S.K. and Kwak, D.C. (1991) The enzymatic modification and functionalities of filefish skin collagen. J. Kor. Agic. Chem. Soc., 34, 265-272
29 Kwon, M.C., Qadir, S.A., Kim, H.S. and Ahn, J.H. (2008) UV protection and whitening effects of collagen isolated from outer layer of the squid Todarodes pacificus. J. Kor. Fish. Soc., 41, 7-12
30 Yang, J.H., Lee, Y.C. and Lee, K.H. (2007) Comparison of sanitization process for long-term storage of fresh red pepper. Korean J. Food Sci. Technol., 39, 419-424
31 Fessler, J.H. (1959) Some properties of neutral-salt-soluble collagen. Biochem. J., 76, 452-463
32 Tanzer, M.L. (1973) Cross-linking of collagen. Science, 180, 561-566   DOI
33 Rehn, M., Veikkola, T., Kukk-valdre, E., Makamura, H., IImonen, M., Lombardo, C., Pihlajaniemi, T., Alitalo, K. and Vuori, K. (2001) Interaction of endostatin with intefrins implicated in angiogeneses. Proc. Natl. Acad. Sci., 98, 1024-1029   DOI   ScienceOn
34 Bailey, A.J. and Light, N.D. (1989) Connective tissue in meat and meat products. Elsevier Science Publishers LTD, NY.
35 Salt, J.S., Barnett, P.V., Dani, P. and Williams, L. (1998) Emergency vaccination of pigs against foot-and-mouth disease: protection against disease and reduction in contact transmission. Vaccine, 16, 746-754   DOI   ScienceOn
36 Rich, A. and Crick, F.H. (1991) The molar structure of collagen. J. Mol. Biol., 3, 483-506
37 Cochrane, C.G. (1991) Cellular injury by oxidants. Am. J. Med., 97, 23-30
38 Ashley, W., Masahiro, O., Ralph, JP., Mark, S., Mark, S. and Jack, NL. (2008) Biochemical properties of alligator(Aligator mississippiensis) bone collagen. Comparative Biochem. Physiol (Part B)., 151, 246-249   DOI   ScienceOn