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Enzymatic Synthesis of Diacylglycerol Oil from Glyceryl Mono-oleate and Conjugated Linoleic Acid Using a Stirred-Batch Type Reactor  

Jeon, Mi-Sun (Department of Food Science and Technology, Chungnam National University)
Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
Publication Information
Food Science and Preservation / v.16, no.2, 2009 , pp. 246-252 More about this Journal
Abstract
Diacylglycerol(DAG) was produced by enzymatic esterification of glyceryl mono-oleate(GMO) and conjugated linoleic acid(CLA) in a stirred-batch type reactor. The reaction was catalyzed by lipozyme RMIM(an immobilized lipase from Rizomucor miehei). DAG was isolated by a short-path distillation process and decolorized. DAG oil was composed of 87.3% DAG, 11.4% triacylglycerol(TAG), and 1.5% monoacylglycerol(MAG)(all w/w). Major fatty acids in DAG oil were oleic acid(54%), CLA(31.1%), and linoleic acid(7%). DAG oil iodine,and acid values were 108.8, 2.57, and 1, respectively. The DAG oil solid fat index(SFI) and thermograms were obtained using differential scanning calorimetry.
Keywords
glyceryl monooleate; conjugated linoleic acid; diacylglycerol oil; lipozyme RMIM; short path distillation;
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