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Biological Activities of Methanol Extract of Angelica gigas Nakai  

Park, Kyung-Wuk (Department of Food and Nutrition, Sunchon National University)
Choi, Sa-Ra (Department of Food and Nutrition, Sunchon National University)
Hong, Hye-Ran (Department of Food and Nutrition, Sunchon National University)
Kim, Jae-Yong (Department of Food Science and Technology, Kyungpook National University)
Shon, Mi-Yae (Department of Food and Nutrition, Gyeongsang National University)
Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University)
Publication Information
Food Science and Preservation / v.14, no.6, 2007 , pp. 655-661 More about this Journal
Abstract
The biological activities of methanol extracts of Angelica gigas Nakai, such as antioxidation, anticancer and immuno-activity, were investigated in relation to development of functional foods. Anti-oxidation activity in the methanol extracts were assessed by hydrogen donating activity, reducing power and hydroxyl radical scavenging activity. Activities were dose-dependent over concentrations of 0.1, 0.5 and 1 mg/mL, with thehydrogen donating activity being over 50% at 1 mg/mL concentration. The methanol extracts inhibited the proliferation of SW480 cells in a dose-dependent manner, and chromatin condensation and apoptotic bodies were observed by fluorescence microcopy in the cells treated with the extracts for 24 hr. Caspase-3 activity was also increased in a dose-dependent manner in cells treated with the extracts relative to control cells. The extracts did not induce the proliferation of mouse spleen cells or NO production in macrophage cells (RAW 264.7). These results show that the methanol extract had slight anti-oxidative activity and did not increase immuno-activity, but inhibited proliferation of SW480 through apoptosis via a caspase dependent pathway.
Keywords
Angelica gigas Nakai; antioxidation; cytotoxicity; apoptosis;
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Times Cited By KSCI : 12  (Citation Analysis)
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