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Analysis of Immunomodulating Activities in Methanol Extracts from Several Kinds of Grapes  

Heo, Jin-Chul (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Woo, Sang-Uk (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Kweon, Mi-Ae (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Kim, Bo-Bae (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Lee, Sook-Hee (GyeongSangBukDo Agricultural Research & Extension Services)
Lee, Jin-Man (Department of Food & Biotechnology, Hoseo University)
Choi, Jong-Uck (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Chung, Shin-Kyo (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Lee, Sang-Han (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
Publication Information
Food Science and Preservation / v.14, no.4, 2007 , pp. 419-424 More about this Journal
Abstract
Fruits and vegetable extracts are well-known as healthy foods. Such foods have been used as herbal medicines or traditional therapies for centuries. To assess biological activities in grapes, we examined the immunomodulating activities of water extracts from four kinds of grapes (cultivars Kyoho, Delaware, Campbell, and Niagara). We explored possible antioxidant and anticancer activities using antioxidant assays such as the 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction assay, the ferric iron reducing ability of plasma (FRAP) assay, a cell proliferation assay, an NO inhibition assay, a wound healing assay, and an IL-4/IL-13 elicitation assay. Methanol extracts of grapes were tested. The results showed that each grape extract had potent antioxidant activities. The grape extracts increased cell proliferation and NO production activities in tumor cell lines. IL-4 and IL-13 cytokine levels were decreased in mouse primary spleen cells by treatment with any extract. These results suggest that grape extracts can be used as biomaterials with immunomodulating activities.
Keywords
grape extracts; immune-modulating activities; antioxidant; anticancer; nitric oxide;
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