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Changes in Polysaccharides Content and Cell Morphology of Fomitopsis pinicola Mycelium during Submerged Culture  

Jung Yoo-Kyung (Department of Food Science and Technology, Faculty of Food Industrial Technology, Catholic University of Daegu)
Shin Kyung-Ok (Department of Food Science and Technology, Faculty of Food Industrial Technology, Catholic University of Daegu)
Park Hong-Duok (Department of Medical Life Science, Catholic University of Daegu)
Kim Soon-Dong (Department of Food Science and Technology, Faculty of Food Industrial Technology, Catholic University of Daegu)
Publication Information
Food Science and Preservation / v.13, no.3, 2006 , pp. 397-403 More about this Journal
Abstract
This experiment was conducted to examine the changes in polysaccharide concentration and morphological variation of Fomitopsis pinicola mycelium during submerged-culture in the citrus peel medium (CP). On the 12 days culture, the yields of mycelium and alcohol insoluble substance were 40.21%(w/v) and 6.94%(w/w), respectively, which were much higher than 11.29%(w/v, wet basis) and 3.17%(w/w, wet basis) obtained from YM medium. A large amount of acid soluble polysaccharides was derived from YM medium while a larger amount of alkali soluble polysaccharide was produced from CP medium. Yields of the mycelium were higher when cultured in CP medium However, there was no significant difference in formation of membranous vesicle between mycelia cultured in CP medium and YM medium. It was also observed that the formation of vacuole was closely related to the activation of the multivescular body known as cytolysome. As a result activation of mycelium and cell wall biosynthesis were more accelerated in CP medium.
Keywords
Fomitopsis pinicola; polysaccharide; morphological changes;
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