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Analysis of Antimicrobial Substance isolated from Scutellariae Radix Extract using LC-MS  

Kim Young-Rok (Gyeongsangnamdo Provincial Government Institute of Health & Environment)
Choi Sung-Gil (Department of Food Science and Technology, Institute of Agriculture and Life Sciences, Gyeongsang National University)
Cho Sung-Hwan (Department of Food Science and Technology, Institute of Agriculture and Life Sciences, Gyeongsang National University)
Publication Information
Food Science and Preservation / v.12, no.4, 2005 , pp. 350-354 More about this Journal
Abstract
It was carried out for development of natural antimicrobial on Scutellariae Radix by measuring the general nutritional composition and mineral contents. This study analyzed it with LC-MS to identify antimicrobial activity substance in the Scutellariae Radix extract, its content was analyzed using HPLC. The searching substance was examined antimicrobial activity by disk method. The approximate composition of Scutellariae Radix was $8.93\%$ of moisture, $4.13\%$ of crude ash, $15.30\%$ of crude protein, $3.71\%$ of crude fat, $67.93\%$ of carbohydrate, respectively. Scutellariae Radix was contained high contents of minerals such as K(12,050 mg/kg),. Mg(4,295 mg/kg), remarkably. It was identified that molecular weight 445' peak was baicalin, molecular weight 283' peak was wogonin and molecular weight 269' peak was baicalein on Scutellariae Radix extract Baicalin showed remarkable antimicrobial activity against V. parahaemolyticus and L monocytogenes when examined by disk method, which was estimated antimicrobial activity substance in the Scutellariae Radix extract. The content of baicalin was $0.75\%$(with D.W.) and $0.82\%$(with methanol) in the Scutellariae Radix extract using HPLC.
Keywords
Scutellariae Radix extract; LC-MS; baicalin; wogonin; baicalein;
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