Browse > Article

Effects of Phenolic Compounds of Persimmon Leaves on Antioxidative System and Miscellaneous Enzyme Activities Related to Liver Function in Ethanol-Induced Hepatotoxicity of Rats  

정창주 (조선대학교 식품영양학과)
윤준식 (조선대학교 유전자과학과)
이명렬 (조선대학교 식품영양학과)
Publication Information
Food Science and Preservation / v.11, no.1, 2004 , pp. 79-87 More about this Journal
Abstract
To investigate antioxidative effects of phenolic compounds separated from persimmon leaves(PL)(Diospyros kaki Thunb.) on the ethanol-induced hepatotoxicity in rat, Sprague-Dawley rats weighing 100-150 g were divided into 5 groups; control group(CON), PL(70 mg/kg) administered group(PEl), ethanol(5 mL/kg, 25％) administered group(ETH), PL(70 mg/kg) and ethanol administered group (PE2), and PL(140 mg/kg) and ethanol administered group(PE3), respectively. The antioxidative activity of persimmon leaves decreased in order of ethylacetate>interphase materials>n-butanol>chloroform>n-hexane>water fraction. The growth rate and feed efficiency ratio decreased by ethanol were gradually increased to the adjacent level of CON by administering PL. The serum activities of ALT, alkaline phosphatase and lactic acid dehydrogenase elevated by ethanol were decreased significantly. It was also observed that the activities of SOD, catalase, and GSH-Px of rat liver increased by ethanol were markedly decreased in PL administered group as compared to ETH. The GSH content of liver was decreased by ethanol, but that was increased in PE1 and PE2 compared with ETH as a dose-dependant manner. These results suggested that phenolic compounds separated from persimmon leaves have a possible protective and relievable effect on the ethanol-induced hepatotoxicity in rats.
Keywords
protective effect; hepatotoxicity; TBA-reactants; antioxidative activity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 김진구 . 김광수 (1982) 감엽성분에 관한 연구. 상주농업전문대학 논문집, 21, 95-97
2 Joung, S.Y. Lee, S.J. Sung, N.J. Jo, J.S. and Kang, S.K. (1995) The chemical composition of persimmon (Diospyros kaki, Thumb) leaf tea. J. Korean Soc. Food Nutr., 24, 720-726
3 Glatthaar, B.E. Homig, D.H. and Fladmoe, A.V. (1979) Inhibition of chemical carcinogen induced neoplasia by coumanns and a-angelicalactone. Cancer Res., 37, 1051-1063
4 Song, H.S. Lee, H.K Jang, H.D. Kim, J.I. Park, O.J. Lee, M.S. and Kang, M.H. (1996) Antimutagenic effects of persimmion leaf tea extracts in Sister Chromated Exchange(SCE) Assay System. J. Koraen Soc. Food Nutr., 25, 232-241
5 Buege, J. A. and Aust, S.D. (1969) Microsomal lipid peroxidation. In 'Methods in enzymoogy' Packer, L. (ed.), Academic Press, New York, 27, 502-511
6 Tietze, F. (1969) Enzymatic methods for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem., 27, 502-513   DOI   PUBMED   ScienceOn
7 Lowry, C.H. Rsenbrough, N.J. Farr, A.L. and Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem., 193, 265-275
8 Okamura, H. Mimura, A. Yakou, Y. Niwano, M. and Takahama, Y. (1993) Antioxidant activity of tannins and flavonoids in Eucalyptus rostrata. Phytochemistry, 33, 557-563   DOI   ScienceOn
9 Lieber, C.S. (1986) The influence of alcohol on nutritional status. Nutr. Rev., 46, 24-35   DOI   ScienceOn
10 Zimmerman, H.J. (1981) Chemical hepatic injury and its detection, in toxicology of the liver, Plaa, G. L. and Hewitt, W. R., eds., Raven press, 22, p.1-10
11 McCord, J.M. and Fridovich, I. (1969) Superoxide dismutase an enzymatic function for erythrocuprein (hemocuprin), J. Biol. Chem., 244, 6049-6059
12 Lieber, C.S. (1980) Increased loss and decreased synthesis of hepatic glutathione after acute alcohol administration. Phamacol. Biochem. Behav., 13, 17-29   DOI   ScienceOn
13 Fridovich, I. (1986) Bilogical effects of the superoxide radical, Arch. Biochem. Biophys., 247, 1-45   DOI   PUBMED   ScienceOn
14 Robbins, S.L. and Kumar, V,K. (1987) Basic pathology, Saunders, New York, p.14-21
15 Fujji, M. Ohmachi, T. Sagarni, I. and Watanabe, M. (1985) Liver microsomal drug metabolism in ethanol treated hamsters. Biochem. Pharmacol., 34, 3881-3890   DOI   ScienceOn
16 Park, W.K. Yoo, Y.H. and Hyun, J.S. (1975) Study on the manufacture jam with korean persimmon. J. Korean Soc. Food Nutr., 4, 25-29
17 Sohn, T.H. Choi, J.U Cho, R.K. and Soong, H.M. (1978) Studies on the Utilization of Persimmons (part 5). Investigation of the Optimum Thickness of Film Bag for Polyethylene Film Storage of Astringent Variety. Korean J. Food Sci. Technol., 10, 73-77
18 Crapo, C.H. McCord, J.M and Fridovich, I. (1978) Preparation and assay of superoxide dismutase, Methods enzymol. ed. Fleischer S and Packer L., Academic press, New York, 53, p.382-422
19 Choi, U. Shin, D.H. Chang, Y.S. and Shin, J.I. (1992) Screening of Natural Antioxidant from Plant and Their Antioxidative Effect. Korean J. Food Sci. Technol., 24, 142-148
20 Stocker, R. and Frei, B. (1991) Endogenous antioxidant defences in human blood plasma. In oxidative stress, Sies, H. ed. Academic Press, New York, 60, p.213-224
21 Bate-Smith, E.C. (1975) Phytochemistry of proanthocyannidins. Phytochemistry, 14, 1107-1118   DOI   ScienceOn
22 Aykac, G. Usnal, M. Yalcin, S. Kocak-Toker,N. Sivas, A. and Oh, H. (1985) The effects of chronic ethanol ingestion on hepatic lipid peroxide, glutathione peroxide, and glutathione transferase in rats. Toxico., 36, 73-77
23 Hong, J.H. Lee, G.M. and Hur. S.H. (1996) Production of vinegar using deteriorated deastringent persimmons during low temperature storage. J. Korean Soc. Food Nutr., 25, 123-128
24 Choi, C.H. (1990) The Aroma Components of Duchung Tea and Persimmon Leaf Tea. Korean J. Food Sci. Technol., 22, 405-411
25 Fridovich, I. (1989) Superoxide dismutases, J. Biol. Chem., 264, 7761-7770
26 Choi, S.W. Kang, W.W. Chung, S.K. and Cheon, S.H. (1996) Antioxidative activity of flavonoids in persimmon leave. Foods and Biotechnology, 5, 119-127
27 Kameda, K. Takaku, T. Okuda, H. and Kimura, Y. (1987) Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-coverting enzyme activity. J. Natl. Products, 50, 680-692   DOI
28 Recknagel, R.O. Glende, E.A, and Hruszkewyez, A.M. (1977) Chemical mechanisms in carbon tetrachloride toxicity. in 'Free radicals in biology' Pryor, W. A. (ed.), Academic Press, New York, p.91-110
29 Park, J.O. and Kim, H.J. (1976) Studies on Ascorbic Acid contents in Persinunon leaves tea by different cooking methods. Journal of the Korean Home Economics Association, 17, 31-61
30 Girotti, A.W. Thomas, J.P. and Jordan, J.E. (1985) Inhibitory effect of Zinc( II) on free radical lipid peroxidation in erythrocyte membranes. J. Free Rad. Bio. Med., 1, 395-404   DOI   ScienceOn
31 Jones,D. P. Eklow, L. Thor, H. and Orrenius, S. (1981) Metabolism of hydrogen peroxide in islated hepatocytes : relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated $H_2O_2.$ Arch. Biochem. Biophys., 210, 505-513   DOI   ScienceOn
32 성종환 (1986) 감시의 자연탈삽현상 및 탄닌물질의 문포. 박사학위논문, 경북대학교
33 Joslyn, M.A. (1970) Methods in food analysis. Academic Press, New York, p.701
34 Su, J.D. Osawa, T. Kawakishi, S. and Namiki, M. (1988) Tannin antioxidants from Osbeckia chinensis. Phytochemistry, 27, 1315-1322   DOI   ScienceOn
35 Videlia, L.A. Femanded, V, Valenzuela, A. and Ugarte, G. (1980) The effects of chronic alcohol on glutathione in islated hepatocytes. Biochem. J., 188, 549-556   DOI
36 Aebi, H. (1974) Catalase, Methods of enzymatic analysis, Bergmeyer HU, Bergmeyer, J and Grabi, M., eds. 3rd ed., Verlag. chemie., 2, p.673-688
37 Uchida, S. Edamatsu, R. Hiramatsu, M. Mori, A Nonaka, G. Nishioka, I. Niwa, M. and Ozaki, M. (1987) Condensed tannins scavenge active oxygen free radicals. Med. Sci. Res., 15, 831-841
38 Park, Y.J. Kang, M.H. Kim, J.I. Park, O.J. Lee, M.S. and Jang, H. (1995) Changes of viarnin C and superoxide dismutase(SOD)-like activity of persinunon leaf tea by processing method and extraction condition. Korean J. Food Sci. Technol., 27, 281-285
39 Yoshikawa, T. and Kondo, M. (1989) Free radical lipid peroxidation and vitamin E in liver injury. In 'Handbook of free radical and antioxidants in biomedicine' Jaine, M., Alexander, T. and Qintanihala, H.(eds), Weber, CRC Press., 2, p.l67-178
40 Vina, J. Estrella, J.M. Guerro, C. and Romero, F.J. (1980) Effects of ethanol on glutathione concenturation in islated hepatocytes. Biochem. J., 188, 549-558   DOI
41 Kim, B.G. Rhew, Y.H. Choe, B.S. Chung, H.Y Park, K.Y. and Rhee, S.H. (1993) Effect of selected persimmon leaf components aganist Sarcoma 180 induced tumor in mice. J. Korean Soc. Food Nutr., 22, 307-315
42 Moon, S.H. Kim, J.O. Rhee, S.H. Park, K.Y. Kim, K.H. and Rhew, T.H. (1993) Antimutagenic effects and compounds identified from hexane fraction of persimmon leaves. J. Korean Soc. Food Nutr., 22, 307-318
43 Lieber, C.S. (1980) Interaction of ethanol witr drug, hepatotoxic agent, carcinogen and vitamins. Alcoholism, 25, 157-164
44 차원섭 . 김광수 (1984) 시업차 제조방법이 제품의 품질에 미치는 영향. 상주농업전문대학 논문집, 23, 109-111
45 Flohe, L. Wolfgng, A. and Gunzler, W.A. (1984) Assay of glutathine peroxidase. In Methods in enzymatic analysis. Packer. L. eds. Academic Press, Inc., New York, p.l14-124
46 Shin, S.R. Moon, K.D. Lee, K.H. and Kim, KS. (1991) Change in the enzyme activities pectines and structure of persimmon fruit during softening., J. Korean Soc. Food Nutr., 22, 611-616
47 Lee, T.B. (1982) Illustrated Flora of Korea. Hayngmunsa, Seoul., 612
48 Hong, Y.S. Ham, Y.A. and Sung, N.E. (1984) The effects of vitamin A and E on lipid peroxidation administered rat liver microsomes. Ewha Med., 7, 3-9   DOI
49 Park, W.K. and Yoo, Y.H. (1976) Effect of gas concentration during the storage of persimmon. J. Korean Soc. Food Nutr., 5, 11-17
50 Blois, M.S. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1206   DOI   ScienceOn
51 Plaa, G.L. and Wistschin, H. (1976) Chemicals, drugs and lipid peroxidations. Am.. Rev. Toxicol. Pharmacol., 16, 125-131
52 Okonogi, T. Hattori, Z. Ogiso, A. and Mitsui, S. (1970) Detoxification by persimmon tannin of snake venoms and bacterial toxins. Toxico., 17, 524-535
53 Fridovich, I. (1975) Superoxide dismutase, Annu. Rev. Biochem., 44, 147-151   DOI   PUBMED   ScienceOn
54 Krenitsky, T.A. (1973)Xanthine oxidase and aldehyde oxidase in purine and purine analogue metabolism. Exp. Med. Biol. p.151-169
55 Lee, S.J. (1983) lllustrated Botanical List, Komunsa, Seoul, p.1016
56 Chung, S.H. Moon, K.D. Kim, J.K and Seong, J.H. (1994) Change of chemical components in persimmon leaves during growth for processing persimmon leaves tea. Korean J. Food Sci. Technol., 26, 141-146
57 Lieber, C.S, (1994) Alcohol and the liver. Gastro., 106, 1085-1097   DOI
58 Smuckler, E.A. and Koplitz, M. (1969) The effects of carbon tetrachloride and ethione on RNA synthesis in vivo and in isolated rat liver nuclei. Arch. Biochem. Biophs., 132, 62-71   DOI   ScienceOn
59 Kirigaya, N. Kato, H. and Fujimarki, M. (1971) Studies on antioxidant activity of non-enxymatic browning reactions (lll). Fractionation of browning reaction solution between ammonoa and D-glucose and antioxidant activity of the resulting fractions. J. Agr. Chem. Soc. (Japan), 45, 292-301
60 Lieber, C.S. and DeCarli, L.M. (1970) Hepartic microsomal ethanol oxidizing system : In vitro characteristics and adaptive properties in vivo. J. Biol. Chem.,. 245, 2505-2516
61 Downry, J.M. Miura, Y. Eddy, L.J. Chambers, D.E., Mellen, T. Hearse, DJ. and Yellon, D.M. (1987) Xanthine oxidase is not a source of free radicals in the ischemic rabbit heart. J. Mol. Cell Cardiol., 19 ,1053-1067   DOI   ScienceOn
62 Lieber, C.S. and DeCarli, L.M. (1986) The feeding of ethanol in liquid diet. Alcoholim : Clin. Exp. Res., 10, 550-560   DOI   ScienceOn
63 Fred, J. Yost, J. and Fridovich, I. (1976) Superoxide and hydrogen peroxide in oxygen damage. Arch. Biochem. Biophys., 175, 514-519   DOI   ScienceOn
64 Dolphin, D. Forman, A. Berg, D.C. Fajce, J. and Felton, R, H. (1971) Compounds I of catalase and horeradish peroxidase. Proc. Natl. Acad. Sci. USA, 68, 614-623   DOI   ScienceOn