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Effect of the Biological Activities of Ethanol Extract from Korean Traditional Kochujang Added with Sea Tangle (Laminaria longissima)  

함승시 (강원대학교 바이오산업공학부)
최승필 (강원대학교 바이오산업공학부)
오상화 (강원대학교 바이오산업공학부)
이득식 (동해대학교 관광외식산업학과)
Publication Information
Food Science and Preservation / v.9, no.1, 2002 , pp. 1-7 More about this Journal
Abstract
This study was carried out to investigate antimutagenic and anticancer effects of Korean traditional Kochujang added with sea tangle (Laminuia longissima). Most of the mineral content in Kochujang added with sea tangle was increased when compared to traditional Kochujang. In the Ames test, the inhibition rate of ethanol extract (200 $\mu\textrm{g}$/plate) of Kochujang added with 5% sea tangle in the S. typimurium TA100 strain showed 87.2% against the mutagenesis induced by MNNG. And also, the inhibition rate of ethanol extract (200 $\mu\textrm{g}$/plate) of Kochujang added with 5% sea tangle in the S. typhimurium TA98 and TA100 strains was 62.9% and 71.6%, respectively, against the mutagenesis induced by 4NQO. The suppression under the same condition against B($\alpha$)P and Trp-P-1 in the TA98 and TA100 strains showed 70.2% and 80.8%, and 62.9% and 73.1%, respectively. In the anticancer effects, the treatment of 1.0 mg/mL Kochujang added with 5% sea tangle showed strong cytotoxicity of 78.6% and 79.4% against KATOⅢ and HepG2, respectively.
Keywords
sea tangle; kochujang; cytotoxicity; micronucleus;
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1 Jung. Y.C., Choi, W.J., W.J., Oh, N.S. and Han, M.S. (1996) Distribution and physiological characteristics of yeasts in traditional and commercial kochujang. Kor. J. Food Sci. Technol., 28, 253-259
2 Lee. K.S and Kim, D.H. (1985) Trial manufacture of low salted kochuzang (red pepper soybean paste) by the addition of alcohol. Kor. J. Food Sci. Technol., 17, 146-154
3 Lee, S.K., Lee, T.S. and Nam, S.H. (1978) Studies on the effect of garlic on the enzyme productihon and growth of Aspergillus oryzae. Kor. J. Agric. Chem. Soc., 21, 123-130
4 Colliec, S., Fischer, A.M., Tapon-Bretaudiere, J., Boisson, C., Durand, P. and Jozefonvicz, J. (1991) Anticoagulant properties of a fucoidan fraction. Thromb. Res., 64, 143-154   DOI   ScienceOn
5 Ahn, J.M. and Song, Y.S. (1992) Physico-chemical and sensory characteristic of cakes added sea Mustard and sea tangle powder. Kor. J. Soc. Food Nutr., 28, 534-541
6 Scudiero, D.A., Shoemaker, R.H., Paul, K.D., Monks, A., Tiemey, S., Nofziger, T.H., Currens, M.J., Seniff, D. and Boyd, M.R. (1988) Evaluation of a soluble tetrazolium/ formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res., 48, 4827-4836
7 Martin, A. and Martin, C. (1997) Comparision of 5 microplate colorimetric assay for in vitro cytotoxicidy testing and cell proliferation assays. Cytotechnology, 11, 49-54   DOI   ScienceOn
8 Yoon, K.D., Kwon, D.J., Hong, S.S., Kim, H.S., Chung, K.S. (1996) Inhibi-tory effect of soybean and fermented soyben products on the chemically induced mutagenesis. Kor. J. Appl. Microbiol. Biolechnol., 24, 525-528
9 Tiisala, S., Majuri, M-L., Carpen, O. and Renkonen, R. (1994) Genistein enhances the ICAM-1 and its counter-receptors. Biochim. Biophys. Res. Commun., 203, 443   DOI   ScienceOn
10 Shin. D.H., Kim, D.H., Choi, U., Lim, D.K. and Lim, M.S. (1996) Studies in taste components of traditional kochujang. Kor. J. Food Sci. Technol., 28, 152-156
11 Ji, W.D., Jeong. M.S., Choi, U.K., Choi, D.H. and Chung, Y.G. (1998) Growth inhibition of garlic (Allium sativum L.) juice on the microorganisms. Agric. Chem. and Biothechnol., 41, 1-5
12 Cho. H.O., Kim, J.G., Lee, H.J., Kang, J.H. and Lee, T.S. (1981) Brewing method and composition of traditional kochuzang (red pepper paste) in junrabookdo area. Kor. J. Agric. Chem. Soc., 24, 21-28
13 Kim, D.S. and Park, Y.H. (1985) Uronic acid composition, block structure and some related properties of alginic acid. Kor. J Fish. Soc., 18, 29-36
14 Shin, D.H., Kim, D.H., Choi, U., Lim, D.K. and Lim, M.S. (1996) Studies on the physicochemical characteristics of traditional kochujang. Kor. J. Food Sci. Technol., 28, 157-161
15 Cho, D.M., Kim, D.S., Lee, D.S., Kim, H.R. and Pyeun, J.H. (1995) Trace components and functional saccharides in seaweed-1. Kor. J. Fish. Soc., 28, 49-59
16 최청 (1998) 장류의 생리활성과 산업화 전망. 제6회 인제식품과학 FORUM 논총. p.122-151
17 농촌진흥청 농업생활연구소 (1996) 식품성분표, 제5개정판
18 Yahagi, T., Nagao, M., Seino, Y., Matsushima, T., Sugimura, T. and Okada, M. (1977) Mutagenicities of N-nitrosoamines on Salmonella. Mutation Res., 48, 121-130   DOI
19 Lee, K.H., Lee, M.S. and Park, S.O. (1976) Studies on the microflora and enzymes influencing on Korean native kochujang (red pepper soybean paste) aging. Kor. J. Agic. Chem. Soc., 19, 82-92
20 AOAC (1995) Official Methods for Analysis. 16th ed., Association of Official Analytical Chemists, Washington, D.C., Ch. 27, p.31
21 Chung, K.S., Yoon, D.J., Hong, S.S. and Choi, S.Y. (1997) Cytotoxity of fermented soybean products with various tumor cell using MTT assay. Kor. J. Appl. Microbool. Biotechnol., 25, 477-482
22 食品醫藥品安全廳 (2000) 食品公典(別冊). 문영사, 서울, p.69-72, p.273-277
23 Sheo, H.J. (1999) The antibacterial action of garlic, onion, ginger and red pepper juice. Kor. J. Soc. Food Sci. Nutr., 28, 94-99
24 Lee, K.S., Moon. C.O., Baek, S.H. and Kim, D.H. (1986) Effect of garlic on quality of barley kochuzang brewed with whole red pepper. Kor. J. Appl. Microbiol. Bioeng., 14, 225-232
25 Kim, D.H. and Lee, J.S. (2001) Effect of condiments on ehe physicochemical characteristics of traditional kochujang during fermentation. Kor. J. Food Sci. Technol., 33, 353-360
26 Cho, D.M., Kim, D.S., Lee, D.S., Kim, H.R. and Pyeun, J.H. (1995) Trace omponents and functional saccharides in seaweed-2. Kor. J. Fish. Soc., 28, 270-278