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http://dx.doi.org/10.3746/jkfn.2015.44.9.1286

Acute and Subchronic Toxicity of Gamma-Irradiated Orange  

Jung, Da-Woon (Department of Food Science and Nutrition & The Korean Institute for Nutrition, Hallym University)
Huang, Yu-Hua (Department of Food Science and Nutrition & The Korean Institute for Nutrition, Hallym University)
Choi, Geun-Pyo (Department of Food Processing and Bakery, Gangwon Provincial College)
Kang, Il-Jun (Department of Food Science and Nutrition & The Korean Institute for Nutrition, Hallym University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.9, 2015 , pp. 1286-1294 More about this Journal
Abstract
The acute and subchronic toxicity of 1 kGy gamma-irradiated orange was evaluated in ICR mice. For acute toxicity, groups of 30 male and 30 female ICR mice were orally administered 1 kGy gamma-irradiated orange (0, 1,000, and 2,000 mg/kg). The mortality, clinical sign, body weight changes, and necropsy findings of ICR mice were observed for 14 days. No significant changes in body weight or abnormal gross findings were observed in relation to 1 kGy gamma-irradiated orange. Hematological and serum biochemical parameters were within normal ranges. According to the results, 1 kGy gamma-irradiated orange had no special toxic effects in male and female ICR mice at 2,000 mg/kg. For subchronic toxicity, groups of 36 male and 36 female ICR mice were given a diet of 1 kGy gamma-irradiated orange for 13 weeks (control, non-irradiated, and irradiated imported orange). During the experimental period, mortality, clinical signs, body weight change, food consumption, organ weight, and histopathological examination did not show any changes in comparison to the control group. Several hematological and serum biochemical parameters showed statistically significant changes, but these changes were within normal range. These results indicate that 1 kGy gamma-irradiated orange did not cause any toxic effects in male and female ICR mice and therefore can be considered as safe.
Keywords
acute toxicity; subchronic toxicity; gamma-irradiation; orange; safety;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Pala CU, Toklucu AK. 2013. Microbial, physicochemical and sensory properties of UV-C processed orange juice and its microbial stability during refrigerated storage. LWTFood Sci Technol 50: 426-431.
2 Moon H, Lee HK, Park H. 2013. Impacts of the KORUS FTA's orange import tariff-cut on domestic fruit prices. Korean J Agricultural Economics 54: 15-38.
3 Kang HJ, Chung HS, Jo DJ, Byun MW, Choi SJ, Choi JU, Kwon JK. 2003. Effects of gamma radiation and methyl bromide fumigation on physiological and chemical quality of apples. Korean J Food Preserv 10: 381-387.
4 UNEP. 1994. Montreal protocol on substances that deplete the ozone layer. Report of the Methyl Bromide Technical Options Committee, Nairobi, Kenya. p 294.
5 Kyung EJ, Kim KH, Yook HS. 2014. Quality characteristics of gamma irradiated-imported orange during storage at room temperature (20$^{\circ}C$). Korean J Food & Nutr 27: 183-193.   DOI
6 Ko J, Ma Y, Song KB. 2005. Effect of electron beam irradiation on the microbial safety and qualitied of sliced dried squid. J Korean Soc Food Sci Nutr 34: 433-437.   DOI
7 Byun MW, Yook HS. 2003. Internal and external situation of irradiation technology utilization in the food and public health industry. Korean J Food Preserv 10: 106-123.
8 Byun MW, Yook HS, Jo SK, Chong YJ. 1996. Status and prospects of food irradiation technology in Korea. J Food Sci Nutr 1: 262-268.
9 Kume T, Furuta M, Todoriki S, Uenoyama N, Kobayashi Y. 2009. Status of food irradiation in the world. Radiat Phys Chem 78: 222-226.   DOI
10 Byun MW. 1997. Application and aspect of irradiation technology in food industry. Food Science and Industry 30(1):89-100.
11 Jo DJ, Kown JH. 2003. Detection of radiation induced markers in oranges imported from united states of America. J Korean Soc Food Sci Nutr 32: 1-7.   DOI
12 Lee JW. 2009. International cooperation for establishing SOP on quarantine management of irradiated food in international trade. Korea Atomic Energy Research Institute, Daejeon, Korea. p 1-3.
13 Nam HS, Kim KE, Yang JS, Ly SY. 2000. Food majoring college students' knowledge and acceptance of irradiated food. Korean J Dietary Culture 15: 269-277.
14 Wolford ST, Schroer RA, Gohs FX, Gallo PP, Brodeck M, Falk HB, Ruhren R. 1986. Reference range data base for serum chemistry and hematology values in laboratory animals. J Toxicol Environ Health 18: 161-188.   DOI
15 Delincee H. 1998. Detection of irradiated food: DNA fragmentation in grapefruits. Radiat Phys Chem 52: 135-139.   DOI
16 Song HP, Shin EH, Yun HJ, Jo C, Kim D. 2009. Establishing the genotoxicological safety of gamma-irradiated egg white and yolk. Korean J Food Preserv 16: 782-788.
17 Jeon YE, Yin XF, Kim TK, Kang IJ. 2013. Safety evaluation of 30 kGy-irradiated Dakgalbi. J Korean Soc Food Sci Nutr 42: 1475-1481.   DOI
18 Huang YH, Jung DW, Kang IJ. 2014. Genotoxicological safety evaluation of imported oranges irradiated with ionizing energy. J Korean Soc Food Sci Nutr 43: 909-915.   DOI
19 WHO. 1994. Safety and nutritional adequacy of irradiated food. Geneva, Switzerland. p 150.
20 Yin XF, Jeon YE, Kim TK, Shim JH, Kang IJ. 2012. Toxicity evaluation of irradiated Tarakjuk for three months. J Korean Soc Food Sci Nutr 41: 1534-1539.   DOI
21 Jeon YE, Kim HM, Lee JW, Byun MW, Kang IJ. 2008. Toxicity of 30 kGy irradiated cereal powders for three months. J Korean Soc Food Sci Nutr 37: 1264-1270.   DOI