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http://dx.doi.org/10.3746/jkfn.2015.44.6.809

Free Radical Scavenging Effect and Oxidative Stress Protective Activity of Domestic Processed Polygoni Multiflori Radix  

Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology)
Kim, Jun Young (Busan Regional Korea Food and Drug Administration, Ministry of Food and Drug Safety)
Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University)
Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology)
Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology)
Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology)
Lee, Jin Hwan (National Institute of Chemical Safety, Ministry of Environment)
Kim, Yun-Geun (Sancheong Oriental Medicinal Herb Institute)
Ko, Keon Hee (Sancheong Oriental Medicinal Herb Institute)
Goo, Young-Min (Sancheong Oriental Medicinal Herb Institute)
Oh, Kyeong Yeol (Sancheong Oriental Medicinal Herb Institute)
Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.6, 2015 , pp. 809-815 More about this Journal
Abstract
In this study, we confirmed biological compounds from methanol (MeOH) extract of processed Polygoni Multiflori Radix (PPMR), and the radical scavenging effect and oxidative stress protective activity of MeOH extract of PPMR were investigated under in vitro conditions using LLC-$PK_1$ renal epithelial cells. In HPLC analysis, MeOH extract of PPMR contained four species of biological compounds named 2,3,5,4'-tetrahydroxystilbene 2-O-${\beta}$-D-glucoside, emodin, chrysophanol, and rhein. 2,3,5,4'-Tetrahydroxystilbene 2-O-${\beta}$-D-glucoside was detected as the main compound in PPMR as 115.02 mg/kg. MeOH extract of PPMR showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging activities in a concentration- dependent manner. In particular, upon $50{\mu}g/mL$ of PPMR extract treatment, DPPH, ABTS, and hydroxyl radical scavenging activities were approximately 48.4%, 57.9%, and 81.2%, respectively. LLC-$PK_1$ cell viability declined in response to oxidative stress induced by pyrogallol, sodium nitroprusside (SNP), and morpholinosydnonimine (SIN-1) generators of NO, $O_2{^-}$, and $ONOO^-$, respectively. However, MeOH extract of PPMR significantly and dose-dependently inhibited oxidative-stressed LLC-$PK_1$ cell cytotoxicity. In fact, upon $50{\mu}g/mL$ of PPMR extract treatment, LLC-$PK_1$ cell viabilities were approximately 82.1%, 89.1%, and 77.6% compared to stress levels induced by pyrogallol, SNP, and SIN-1, respectively.
Keywords
processed Polygoni Multiflori Radix; biological compounds; radical scavenging activity; LLC-PK1 cell; oxidative stress;
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Times Cited By KSCI : 8  (Citation Analysis)
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