Browse > Article
http://dx.doi.org/10.3746/jkfn.2015.44.10.1583

Comparison of Protective Effects of Young and Ripened Persimmon Extracts against Inflammatory Stress Induced by Deoxycholic Acid in Small Intestinal Cells  

Kim, Leeseon (Department of Food Science and Technology, Seoul National University of Science and Technology)
Kwon, Oran (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.10, 2015 , pp. 1583-1587 More about this Journal
Abstract
Bile acids are endogenous metabolites that aid in the digestion and absorption of ingested fat and fat-soluble vitamins. However, high concentrations of deoxycholic acid (DCA) in the colon are associated with high incidence of colorectal cancer. In the present study, the binding of persimmon extracts to DCA in order to decrease inflammatory stress induced by DCA in a small intestinal epithelial cell line, Caco-2, was investigated. Young and ripened persimmons were extracted with distilled water (DW), ethanol, and acidic ethanol. Further, DW extract residue was re-extracted with acidic ethanol. Of the obtained extracts, acidic ethanol extract of young persimmon showed the highest bile-acid binding capacity. Moreover, acidic ethanol extract of young persimmon significantly inhibited nitric oxide production in Caco-2 cells stimulated with DCA and prevented significant reduction of trans-epithelial electric resistance. Based on these results, acidic ethanol extract of young persimmon can be used as a functional ingredient to enhance gastrointestinal health.
Keywords
Caco-2; deoxycholic acid; inflammation; tannin; young persimmon;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Da Silva M, Jaggers GK, Verstraeten SV, Erlejman AG, Fraga CG, Oteiza PI. 2012. Large procyanidins prevent bile-acid-induced oxidant production and membrane-initiated ERK1/2, p38, and Akt activation in Caco-2 cells. Free Radic Biol Med 52: 151-159.   DOI
2 Moschetta A, Portincasa P, van Erpecum KJ, Debellis L, Vanberge-Henegouwen GP, Palasciano G. 2003. Sphingomyelin protects against apoptosis and hyperproliferation induced by deoxycholate: potential implications for colon cancer. Dig Dis Sci 48: 1094-1101.   DOI
3 Kim HJ, Park TS, Jung MS, Son JH. 2011. Study on the anti-oxidant and anti-inflammatory activities of sarcocarp and calyx of persimmon (Cheongdo Bansi). J Appl Biol Chem 54: 71-78.   DOI
4 Erlejman AG, Fraga CG, Oteiza PI. 2006. Procyanidins protect Caco-2 cells from bile acid- and oxidant-induced damage. Free Radic Biol Med 41: 1247-1256.   DOI
5 Rakic S, Petrovic S, Kukic J, Jadranin M, Tesevic V, Povrenovic D, Siler-Marinkovic S. 2007. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. Food Chem 104: 830-834.   DOI
6 Hayes JE, Allen P, Brunton N, O'Grady MN, Kerry JP. 2011. Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical product: olive leaf extract (Olea europaea L.), lutein, sesamol and ellagic acid. Food Chem 126: 948-955.   DOI   ScienceOn
7 Mandrioli R, Mercolini L, Ferranti A, Fanali S, Raggi MA. 2011. Determination of aloe emodin in Aloe vera extracts and commercial formulations by HPLC with tandem UV absorption and fluorescence detection. Food Chem 126: 387-393.   DOI
8 Takekawa K, Matsumoto K. 2012. Water-insoluble condensed tannins content of young persimmon fruits-derived crude fibre relates to its bile acid-binding ability. Nat Prod Res 26: 2255-2258.   DOI
9 Trautwein EA, Kunath-Rau A, Erbersdobler HF. 1999. Increased fecal bile acid excretion and changes in the circulating bile acid pool are involved in the hypocholesterolemic and gallstone-preventive actions of psyllium in hamsters. J Nutr 129: 896-902.
10 Epe B, Ballmaier D, Roussyn I, Briviba K, Sies H. 1996. DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res 24: 4105-4110.   DOI
11 Bogdan C. 2001. Nitric oxide and the immune response. Nat Immunol 2: 907-916.   DOI
12 Clark JA, Doelle SM, Halpern MD, Saunders TA, Holubec H, Dvorak K, Boitano SA, Dvorak B. 2006. Intestinal barrier failure during experimental necrotizing enterocolitis: protective effect of EGF treatment. Am J Physiol Gastrointest Liver Physiol 291: G938-G949.   DOI
13 Hackam DJ, Upperman JS, Grishin A, Ford HR. 2005. Disordered enterocyte signaling and intestinal barrier dysfunction in the pathogenesis of necrotizing enterocolitis. Semin Pediatr Surg 14: 49-57.   DOI
14 Halpern MD, Holubec H, Saunders TA, Dvorak K, Clark JA, Doelle SM, Ballatori N, Dvorak B. 2006. Bile acids induce ileal damage during experimental necrotizing enterocolitis. Gastroenterology 130: 359-372.   DOI
15 Lee YC, Sa YS, Jeong CS, Suh KG, Choi HS. 2001. Anticoagulating activity of persimmon and its processed foods. J Korean Soc Food Sci Nutr 30: 949-953.
16 Matsumoto K, Kadowaki A, Ozaki N, Takenaka M, Ono H, Yokoyama S, Gato N. 2011. Bile acid-binding ability of kaki-tannin from young fruits of persimmon (Diospyros kaki) in vitro and in vivo. Phytother Res 25: 624-628.   DOI
17 Seong JH, Han JP. 1999. The qualitative differences of persimmon tannin and the natural removal of astringency. Korean J Postharvest Sci Technol 6: 66-70.
18 Matsuo T, Ito S. 1978. The chemical structure of kaki-tannin from immature fruit of the persimmon (Diospyros kaki L.). Agric Biol Chem 42: 1637-1643.   DOI
19 Kim SG, Lee YC, Suh KG, Choi HS. 2001. Acetaldehyde dehydrogenase activator from persimmon and its processed foods. J Korean Soc Food Sci Nutr 30: 954-958.
20 Harada M, Sakagami R, Watanabe T, Onitsuka T, Katoh H, Nagai A. 2005. Antibacterial and deodorizing effect of persimmon tannin. Jpn J Conserv Dent 48: 314-319.
21 Shinmoto H, Kimura T, Yamagishi K, Suzuki M. 2002. Antimutagenicity of fruit extract on Trp-P2 induced mutagenicity of Salmonella typhimurium TA98. J Jpn Soc Food Sci Technol 49: 203-206.   DOI
22 Gorinstein S, Bartnikowska E, Kulasek G, Zemser M, Trakhtenberg S. 1998. Dietary persimmon improves lipid metabolism in rats fed diets containing cholesterol. J Nutr 128: 2023-2027.
23 Achiwa Y, Hibasami H, Katsuzaki H, Imai K, Komiya T. 1997. Inhibitory effects of persimmon (Diospyros kaki) extract and related polyphenol compounds on growth of human lymphoid leukemia cells. Biosci Biotechnol Biochem 61: 1099-1101.   DOI
24 Hibino G, Nadamoto T, Fujisawa F, Fushiki T. 2003. Regulation of the peripheral body temperature by foods: a temperature decrease induced by the Japanese persimmon (kaki, Diospyros kaki). Biosci Biotechnol Biochem 67: 23-28.   DOI
25 Sakaguchi T, Nakajima A. 1994. Accumulation of uranium by immobilized persimmon tannin. Sep Sci Technol 29: 205-221.   DOI
26 Seo JH, Jeong YJ, Kim KS. 2000. Physiological characteristics of tannins isolated from astringent persimmon fruits. Korean J Food Sci Technol 32: 212-217.
27 Matsumoto K, Yokoyama S, Gato N. 2010. Bile acid-binding activity of young persimmon (Diospyros kaki) fruit and its hypolipidemic effect in mice. Phytother Res 24: 205-210.
28 Raimondi F, Santoro P, Barone MV, Pappacoda S, Barretta ML, Nanayakkara M, Apicella C, Capasso L, Paludetto R. 2007. Bile acids modulate tight junction structure and barrier function of Caco-2 monolayers via EGFR activation. Am J Physiol Gastrointest Liver Physiol 294: G906-G913.