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http://dx.doi.org/10.3746/jkfn.2011.40.10.1423

Changes in Antigenicity of Gliadin from Medium Flour by Autoclave and Microwave Treatments  

Kwak, Ji-Hee (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Lee, Chung-Jo (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Min-Ji (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Dong-Hyun (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
SunWoo, Chan (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Jung, Seul-A (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kang, Ju-Youn (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Hyun-Jee (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Choi, Jung-Su (Subdivision of Food Science, Kyungnam College of Information and Technology)
Kim, Seong-Won (Dept. of Pediatrics, Busan ST. Mary's Medical Center)
Ahn, Dong-Hyun (Dept. of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.10, 2011 , pp. 1423-1429 More about this Journal
Abstract
This study was conducted to evaluate the effect of physical treatments on the antigenicity of gliadin in medium wheat flour. The wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), and both (10, 30, 50 min/ 5, 10 min), and investigated by SDS-PAGE, immunoblotting and Ci-ELISA using anti-gliadin IgG. The results showed that the binding ability of anti-gliadin IgG to gliadin in wheat flour was slightly decreased when autoclaved or when autoclaved and microwaved. Especially, it was reduced to about 69% after autoclaving for 50 min and 73% after autoclaving for 50 min and microwaving for 5 min. In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. Consequently, there were no considerable changes in using an autoclave alone or in combination with a microwave. These results suggest that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat flour.
Keywords
wheat allergy; gliadin; autoclave; microwave;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Mullin J. 1995. Microwave processing. In New Methods of Food Preservation. Gould GW, ed. Blackie Acad Prof., Glasgow, UK. p 112-134.
2 Venkatachalam M, Teuber SS, Roux KH, Sathe SK. 2002. Effects of roasting, blanching, autoclaving, and microwave heating on antigenicity of almond (Prunus dulcis L.) proteins. J Agric Food Chem 50: 3544-3548.   DOI
3 Hanane K, Kamel Eddine EM, Omar K, Djamel S. 2006. Microwave treatment modify antigenicity properties of bovine milk proteins. Afr J Biotechnol 5: 1267-1270.
4 Kim MJ, Lee JW, Yook HS, Lee SY, Kim MC, Byun MW. 2002. Changes in the antigenic and immunoglobulin E-binding properties of hen's egg albumin with the combination of heat and gamma irradiation treatment. J Food Prot 65: 1192-1195.   DOI
5 Bonomi F, Iametti S, Rasmussen P, Restani P, Rovere P. 2000. Advances in the combined application of enzymatic and physical treatments for reducing food allergenicity. High Pres Res 19: 175-181.   DOI
6 Han GD, Matsuno M, Ikeuchi Y, Suzuki A. 2002. Effects of heat and high-pressure treatments on antigenicity of beef extract. Biosci Biotechnol Biochem 66: 202-205.   DOI
7 Towbin HT, Staehelin T, Gordon J. 1979. Electrophoretic transfers of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.   DOI
8 Lee JW, Park JH, Kim SB, Kim CJ, Hyun CK, Shin HK. 1998. Application of competitive indirect enzyme-linked immunosorbent assay (Ci-ELISA) for monitoring the degree of frozen denaturation of bovine myosin. Int J Food Sci Technol 33: 401-410.   DOI
9 Rumbo M, Chirdo FG, Fossati CA, Anon MC. 2001. Analysis of the effects of heat treatment on gliadin immunochemical quantification using a panel of anti-prolamin antibodies. J Agric Food Chem 49: 5719-5726.   DOI
10 Mondoulet L, Paty E, Drumare MF, Ah-Leung S, Scheinmann P, Willemot RM, Wal JM, Bernard H. 2005. Influence of thermal processing on the allergenicity of peanut proteins. J Agric Food Chem 53: 4547-4553.   DOI
11 Shibata M, Saegusa S, Hosoi T, Ogawa T, Numata K. 2008. The fate of soybean allergens in autoclaved soybeans and 'Edo-ama' miso. Bull Tokyo Metro Agric Fores Res Cen 3: 71-79.
12 Alvarez-Alvarez J, Guillamon E, Crespo JF, Cuadrado C, Burbano C, Rodríguez J, Fernández C, Muzquiz M. 2005. Effects of extrusion, boiling, autoclaving, and microwave heating on lupine allergenicity. J Agric Food Chem 53: 1294-1298.   DOI
13 Varjonen E, Bjorksten F, Savolainen J. 1996. Stability of cereal allergens. Clin Exp Allergy 26: 436-443.   DOI
14 Cuadrado C, Cabanillas B, Pedrosa MM, Varela A, Guillamon E, Muzquiz M, Crespo JF, Rodriguez J, Burbano C. 2009. Influence of thermal processing on IgE reactivity to lentil and chickpea proteins. Mol Nutr Food Res 53: 1462-1468.   DOI
15 Kumagai H, Suda A, Sakurai H, Kumagai H, Arai S, Inomata N, Ikezawa Z. 2007. Improvement of digestibility, reduction in allergenicity, and induction of oral tolerance of wheat gliadin by deamidation. Biosci Biotechnol Biochem 71: 977-985.   DOI
16 DuPont FM, Vensel WH, Chan R, Kasarda DD. 2000. Characterization of the 1B-type $\omega$-gliadins from Triticum aestivum cultivar butte. Cereal Chem 77: 607-614.   DOI
17 Su M, Venkatachalam M, Teuber SS, Roux KH, Sathe SK. 2004. Impact of $\gamma$-irradiation and thermal processing on the antigenicity of almond, cashew nut and walnut proteins. J Sci Food Agr 84: 1119-1125.   DOI
18 Izquierdo FJ, Penas E, Baeza ML, Gomez R. 2008. Effects of combined microwave and enzymatic treatments on the hydrolysis and immunoreactivity of dairy whey proteins. Int Dairy J 18: 918-922.   DOI
19 Maruyama N, Sugiura F, Kishimoto T, Ichise K, Takeuchi Y, Sawada T, Tsuda A, Utsumi S. 1999. Decreased IgEbinding with wheat gluten by deamidation. Biosci Biotechnol Biochem 63: 567-569.   DOI
20 Leszczynska J, Lacka A, Szemraj J, Lukamowicz J, Zegota H. 2003. The influence of gamma irradiation on the immunoreactivity of gliadin and wheat flour. Eur Food Res Technol 217: 143-147.   DOI
21 Leszczynska J, Lacka A, Szemraj J, Lukamowicz J, Zegota H. 2003. The effect of microwave treatment on the immunoreactivity of gliadin and wheat flour. Eur Food Res Technol 217: 387-391.   DOI
22 Park JY, Ahn JY, Hong HO, Hahn YS. 2004. Reduction of allergenicity of wheat flour by enzyme hydrolysis. Korean J Food Sci Technol 36: 152-157.   과학기술학회마을
23 Sungshin Women's University. 2002. Report: Development of hypoallergenic bread for domestic wheat. Ministry of Science and Technology. p 1-43.
24 Son DY, Lee BR, Shon DW, Lee KS, Ahn KM, Nam SY, Lee SI. 2000. Allergenicity change of soybean proteins by thermal treatment. Korean J Food Sci Technol 32: 959-963.   과학기술학회마을
25 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.   DOI
26 Lee JW, Yook HS, Cho KH, Lee SY, Byun MW. 2001. The changes of allergenic and antigenic properties of egg white albumin (Gal d 1) by gamma irradiation. J Korean Soc Food Sci Nutr 30: 500-504.   과학기술학회마을
27 Shin JH, Jeong SG, Han GS, Jang A, Chae HS, Yoo YM, Ahn CN, Lee JW, Jo C, Lee WK, Ham JS. 2008. Reduction of the antigenicity of powdered milk by gamma irradiation. Korean J Food Sci Ani Resour 28: 306-311.   과학기술학회마을   DOI
28 Kim SJ, Kim KBWR, Song EJ, Lee SY, Yoon SY, Lee SJ, Lee CJ, Park JG, Lee JW, Byun MW, Ahn DH. 2009. Changes of pork antigenicity by heat, pressure, sonication, microwave, and gamma irradiation. Korean J Food Sci Ani Resour 29: 709-718.   과학기술학회마을   DOI
29 Kim SM, Park JG, Kim KBWR, Lee JW, Byun MW, Park SM, Ahn DH. 2006. Study on the changes in allergen and allergenicity originated from shrimp by physical treatments. J Korean Soc Food Sci Nutr 35: 990-996.   과학기술학회마을   DOI
30 Han GD, Fan JP, Atsushi S. 2006. Changes of SDS-PAGE pattern and allergenicity of BSA and BGG in beef extract treated with heat and high pressure. J Korean Soc Food Sci Nutr 35: 594-599.   과학기술학회마을   DOI
31 Food and Agriculture Organization of the United Nations. 1995. Report of the FAO Technical Consultation on Food Allergies. Rome, Italy. November 13-14.
32 Li Z, Lin H, Cao L, Khalid J. 2007. Impact of irradiation and thermal processing on the immunoreactivity of shrimp (Penaeus vannamei) proteins. J Sci Food Agr 87: 951-956.   DOI
33 Sampson HA. 1999. Food allergy. Part 1: Immunopathogenesis and clinical disorders. J Allergy Clin Immunol 103: 717-728.   DOI
34 Lee SI. 2006. Food allergy. Safe Food 1: 12-17.
35 Son DY, Yoon KR, Lee SI. 2002. Study of the most common allergic foods in Korea. Korean J Food Sci Technol 34: 885-888.   과학기술학회마을
36 Food balance sheet. 2008. Korea rural economic institute.
37 Korea Food and Drug Administration. Labeling standards for foods etc. KFDA notification No. 2011 (Amendment on Mar. 31, 2010). Accessed Jan. 1, 2011.
38 Shon DW. 2000. Food and allergy. Food Sci Indus 33: 2-9.
39 Falchuk ZM, Gebhard RL, Sessoms C, Strober W. 1974. An in vitro model of gluten-sensitive enteropathy. J Clin Invest 53: 487-500.   DOI   ScienceOn
40 Battais F, Mothes T, Moneret-Vautrin DA, Pineau F, Kanny G, Popineau Y, Bodinier M, Denery-Papini S. 2005. Identification of IgE-binding epitopes on gliadins for patients with food allergy to wheat. Allergy 60: 815-821.   DOI
41 Lee SY. 2004. Food allergy. Korean J Pediatr 47: 240-246.   과학기술학회마을