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http://dx.doi.org/10.3746/jkfn.2007.36.5.521

Antioxidant Effects of Red Ginseng Powder on Liver of Benzo(α)Pyrene-Treated Mice  

Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University)
Lee, Ji-Won (Dept. of Food and Technology, Keimyung University)
Ji, Young-Ju (Dept. of Food and Technology, Keimyung University)
Yu, Me-Hei (Dept. of Food and Technology, Keimyung University)
Park, Jung-Hyun (The Center for Traditional Microorganism Resources, Keimyung University)
Lee, Ki-Dong (Daegu Bio Industry Center, Daegu New Technology Agency)
Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.36, no.5, 2007 , pp. 521-526 More about this Journal
Abstract
The effects of red ginseng powder on hepatotoxicity in $benzo(\alpha)pyrene\;[B(\alpha)P]$-treated mice were investigated. Male ICR mice were pretreated with red ginseng powder (50 or 100 mg/kg/day, for 5 days, intraperitoneally) before treatment with $B(\alpha)P$ (0.5 mg/kg, i.p, single dose). The ability of red ginseng powder to protect against oxidative damage to the mouse liver was examined by determining the level of lipid peroxide, glutathione, and the antioxidant enzyme activities. The glutathione content depleted by $B(\alpha)P$ were significantly increased by red ginseng powder, but elevation of lipid peroxide content induced by $B(\alpha)P$ was decreased by red ginseng powder. The increased activities of superoxide dismutase, catalase and glutathione peroxidase after $B(\alpha)P$-treatment were decreased by the treatment of red ginseng Powder; however, glutathione S-transferase activity depleted by $B(\alpha)P$ were significantly increased. These results suggest that red ginseng powder can protect against $B(\alpha)P$ intoxification through its antioxidant properties.
Keywords
red ginseng powder; $B(\alpha)P$; hepatotoxicity; antioxidant activity;
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1 Marklund S, Marklund CT. 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474   DOI   ScienceOn
2 Kim BS, Kim HK, Choi JW, Lee CK. 1996. The effects of meliae toosendan fructus on liver function. III. Korean J Pharmacog 27: 47-52   과학기술학회마을
3 Jeon BH, Seong GS, Chun SG, Sung JH, Chang CC. 2005. Antioxidantive effects of white ginseng and red ginseng on liver of high fat diet-treated mice. J Ginseng Res 29: 138-144   과학기술학회마을   DOI   ScienceOn
4 Gelboin HV. 1980. Benzo(${\alpha}$)pyrene metabolism, activation and carcinogenesis: role and regulation of mixed-function oxidase and related enzymes. Physiol Rev 60: 1107-1166   DOI
5 Kim CS, Jang DS, Che SY. 2006. Histological charateristics of Korean red ginseng in steaming processes. Korean J Medicinal Crop Sci 14: 36-40   과학기술학회마을
6 Song YB, Kwak YS, Park KH, Chang SK. 2002. Effects of total saponin from red ginseng on activities of antioxidant enzymes in pregnant rats. J Ginseng Res 26: 139-144   과학기술학회마을   DOI   ScienceOn
7 Park SH, Kim JY, Chang JS, Oh EJ, Kim OM, Bae JT, Kim HJ, Hae DJ, Lee KR. 2001. Protective effect of Hericium erinaceus extracts on hepatic injury induced by benzo(${\alpha}$)pyrene in mice. J Korean Soc Food Sci Nutr 30: 928-932   과학기술학회마을
8 Kim DJ, Seug KS, Kim DW, Ko SR, Chang CC. 2004. Antioxidantive effects of red ginseng saponins on paraquot-induced oxidative stress. J Ginseng Res 28: 5-10   과학기술학회마을   DOI   ScienceOn
9 Cheigh HS. 1994. Lipid peroxidation and its nutritional significance. J Korean Soc Food Nutr 23: 867-871   과학기술학회마을
10 Lee CK, Han YN, Kim NY, Choi JW. 2003. The therapeutic effects of Korea red ginseng on carbone tetrachloride- and galactosamine-induced hepatoxicity in rats. J Ginseng Res 27: 11-16   과학기술학회마을   DOI   ScienceOn
11 Lee SE, Lee SU, Bang JK, Yu YJ, Seong RS. 2004. Antioxidant activities of leaf, stem, and root of Panax ginseng C. A. Meyer. Korean J Medicinal Crop Sci 12: 237- 242   과학기술학회마을
12 Sung KS, Chun CC, Kwon YH, Kim KH, Chang CC. 2000. Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice. J Ginseng Res 24: 29-34   과학기술학회마을
13 Choi HJ, Zhang YB, An BJ, Choi C. 2002. Identification of biologically active compounds from Panax ginseng C. A. Meyer. Korean J Food Sci Technol 34: 493-497   과학기술학회마을
14 Ha DC, Ryu GH. 2005. Chemical components of red, white and extruded root ginseng. J Korean Soc Food Sci Nutr 34: 247-254   과학기술학회마을   DOI   ScienceOn
15 Hwang EY, Choi SY. 2006. Quantitative analysis of phenolic compounds in different parts of Panax ginseng C. A. Meyer and its inhibitory effect on melanin biosysthesis. Korean J Medicinal Crop Sci 14: 148-152   과학기술학회마을
16 Kim KH, Sung KS, Chang CC. 2000. Effects of the antioxidative components to ginsenoside in the liver of 40-week-old mice. J Ginseng Res 24: 162-167   과학기술학회마을
17 Aebi H. 1974. Catalase. In Methods of Enzymatic Analysis. Vergmeyer HU, ed. Academic press, New York, USA. Vol 2, p 673-698
18 Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-72   DOI   ScienceOn
19 Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95: 351-358   DOI   ScienceOn
20 Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28: 56-63   DOI
21 Kim HJ, Lee KR. 2003. Effect of Ramaria botrytis methanol extract on antioxidant enzyme activities in benzo(${\alpha}$)pyrene-treated mice. Korean J Food Sci Technol 354: 286-290   과학기술학회마을
22 Sung KS, Chun C, Kwon YH, Chang CC. 2000. Effects of red ginseng component administration on glutathione and lipid peroxidation levels in mice liver. J Ginseng Res 24: 176-182   과학기술학회마을
23 Paglia ED, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169
24 Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferase: the first enzymatic step in mercapturic acid reaction. Anal Biochem 249: 7130-7139
25 Lowry OH, Rosebrough NH, Farr AL, Randall RJ. 1951. Protein measurement with Folin phenol reagent. J Biol Chem 193: 265-275
26 Yoon SH, Park EJ, Oh KH, Chung YG, Kwon OJ. 1993. The effect of lithospermi radix benzo($\alpha$)pyrene-induced hepatotoxicity. J Korean Soc Food Nutr 22: 144-148
27 Lee BC, Park JO, Ryu BH. 1997. Antioxidative effects of silymarin and silybin purified from Silybum marianum on lipid peroxidation. Korean J Food & Nutr 10: 37-43   과학기술학회마을
28 Halliwell B, Gutterridge JM. 1990. Roles of free radicals and catalytic metal ions in human disease. In Methods Enzymol. Fleischer S, Packer L, eds. Academic Press, New York, USA. Vol 186, p 1-12
29 Cohen GM, Freedman RB. 1982. Roles and functions of glutathione. Biochem Soc Trans 10: 78-85   DOI
30 Bompart GJ, Prevot DS, Basacands JL. 1990. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin induced toxicity. Clin Biochem 23: 501-504   DOI   ScienceOn
31 Reynolds J. 1989. The extra pharmacopoeia. 29th ed. The pharmaceutical press, London. p 1613-1620
32 Aktay G, Deliorman D, Ergun E, Ergun F, Yesilada E, Cevik C. 2000. Hepatoprotective effects of turkish folk remedies on experimental liver injury. J Ethernopharm 73: 121-129   DOI   ScienceOn
33 Yun HS, Chang IM, Chi HJ, Lee SY. 1980. Plants with liver protective activities (IV). Korean J Pharmacog 11: 57-60   과학기술학회마을
34 Muriel P, Garciapina T, Perez-alvarez V, Mourelle M. 1992. Silymarin protects against paracetamol-induced lipid-peroxidation and liver damage. J Appl Toxicol 12: 439-442   DOI
35 Sung GS, Chun SG, Chang CC. 2005. Hepatoprotective effects of white and red ginseng extracts on acetaminophen- induced hepatotoxicity in mice. J Ginseng Res 29: 131-137   과학기술학회마을   DOI   ScienceOn