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http://dx.doi.org/10.3746/jkfn.2003.32.3.315

Isolation and Identification of Bioactive Organosulfur Phytochemicals from Solvent Extract of Broccoli  

석대은 (충남대학교 식품영양학과)
김진희 (충남대학교 식품영양학과)
김미리 (충남대학교 약학대학)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.3, 2003 , pp. 315-319 More about this Journal
Abstract
Bioactive organosulfur phytochemicals were isolated from fresh broccoli using methlylene chloride as an extract solvent and identified by GC/MSD analyses. Major organosulfur phytochemicals of broccoli extract were found to be isothiocyanates, which constitute 40.42% of total phytochemicals. The isothiocyanates from broccoli extract were identified to be 3-butenyl, 4-methyl thiobutyl, 4-methylthio-3-butenyl, 5-methylthiopentyl, 2-phenylethyl, 3-methyl sulfinyl propyl, and 4-methylsulfinylbutyl isothiocyanates, of which major isothio cyanates were 3 butenyl isothiocyanate and 4-methylsulfinylbutyl isothiocyanate, constituting about 38.55% of total isothiocyanates present in the solvent extract. Also, nitrites, corresponding to products from enzymatic hydrolysis of glucosinolates were identified as 4-methylthiobutyl, 5-methyl thiopentyl, 2-phenylethyl and 4-methylsulfinylbutyl nitrile. In addition, three sulfides were identified as dimethyl disulfide, dimethyl trisulfide and dimethyl tetrasulfide.
Keywords
broccoli; organosulfur phytochemicals; isothiocyanates; GC/MSD;
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