Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.3.511

Effect of Extract from Paeoniae radix on Rabbit Platelets  

박영현 (순천향대학교 식품영양학과)
손동주 (순천향대학교 식품영양학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.3, 2002 , pp. 511-515 More about this Journal
Abstract
Paeoniae radix has been considered as one of the most important crude drugs used in traditional oriental medicine and has been employed as a circulatory tonic in care of weakness, night sweats, and lumbar pain, etc. Platelet activation plays an important role in thrombosis and haemostasis. Active compounds for the inhibition of platelet activation from Paeoniae radix were extracted and fractionated into five fractions. Its fraction two and three of ethyl acetate extract inhibited the aggregation of washed rabbit platelets induced by collagen. Two active compounds, bezoyloxypaeoniflorin and paeoniflorin, were isolated from fraction two and three by silica gel column and high performance liquid chromatography. The chemical structures were determined by comparison of their proton and carbon nuclear magnetic resonance spectra. Benzoyloxypaeoniflorin showed strong inhibition at the concentration of 100 ug/mL against collagen-induced washed rabbit platelets aggregation. It is suggested that Paeoniae radix has become food material to prevent a cardiovascular disease.
Keywords
Paeoniae radix; platelet aggregation; benzoyloxypaeniflorin; paeoniflorin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 보건복지부. 1997. 1995년도 국민영양조사보고서. 국민영양 97- 10, p 48-51
2 경제기획원 통계청. 1991. 1990년 사망원인 통계연보
3 Matano Y, Okuyama T, Shibata S, Hoson M, Osada H, Noguchi T. 1996. Studies on coumarins of a Chinese drug 'ian-Hu' VII. Structures of new coumarin-glycosides of Zi-Hua Qian-Hu and effect of coumarin-glycosides on human platelet aggregation. Planta Med 50: 135-138
4 Tuminen M, Bohlin L, Rolfsen W. 1991. Effects of calaguala and an active principle, adenosine, on platelet activating factor. Planta Med 58: 306-310   DOI   ScienceOn
5 강병수. 1992. 본초학. 도서출판 수림사, 서울. p 581-583
6 김태정. 1994. 원색도감:약이 되는 한국의 산야초. 국일문화사, 서울. p 684-685
7 지형준, 이상인. 1988. 대한 약전의 한약(생약) 규격집 주해서. 한국메티칼인쇄사, 서울. p 596-597
8 Kang SS, Kim JS, Kim EM, Yun-Choi HS. 1991. Platelet anti-aggregation of Paeony root. Kor J Pharmacogn 22: 215-218
9 Ro HS, Ko WK, Yang HO, Park KK, Cho YH, Lee YE, Park HS. 1999. Isolation of hyperlipidemic substances from methanol extract of Paeoniae radix. J Kor Pharm Sci 29: 55-60
10 Kim YE, Lee YC, Kim HK, Kim CJ. 1997. Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts. Korean J Food & Nutr 10: 141-144.
11 Bang MH, Song JC, Lee SY, Park NK, Baek NI. 1999. Isolation and structure determination of antioxidants from the root of Paeonia lactiflora. J Korean Soc Agric Chem Biotechnol 42: 170-175
12 Goto H, Shimada Y, Akechi Y, Kohta K, Hattori M, Terasawa K. 1996. Endothelium-dependent vasodilator effect of extract prepared from the root of Paeonia lactiflora on isolated rat aorta. Planta Med 62: 436-439   DOI   ScienceOn
13 Liu J. 1983. Effect of Paeonia ovovata 801 on metabolism of thromboxane B2 and arachidonic acid and platelet aggregation in patients with coronary heart disease and cerebral thrombosis. Chung-Huai Chih 63: 477-481
14 Ikeda N, Fukuda T, Jyo H, Shimada Y, Murakami N, Saka M, Yoshikawa M. 1996. Quality evaluation on Paeoniae radix; Ⅰ. Quantitative analysis of monoterpene glycosides constituents of Paeoniae radix by means of high performance liquid chromatography; Comparative characterization of the external figures, processing method and the cultivated areas. Yakugku Zasshi 116: 138-147   DOI
15 Aimi N, Hattori T, Ueyama M, Shinoda S, Miyake Y. 1979. Determination of paeoniflorin in Paeony extract by high performance liquid chromatography. Yakugaku Zasshi 99: 598-601   DOI
16 Shibata S, Nakahara M. 1963. Studies on the constituents of Japanese and Chinese Paeony crude drugs; Ⅷ. Paeoniflorin, a glucoside of Chinese Paeony root (1). Chem Pharm Bull 11: 372-378   DOI
17 Shibata S, Nakahara M, Aimi N. 1963. Studies on the constituents of Japanese and Chinese Paeony crude drugs; Ⅸ. paeoniflorin, a glucoside of Chinese Paeony root (2). Chem Pharm Bull 11: 379-381   DOI
18 Shimizu M, Hayashi T, Morita N, Kimura I, Kimura M. 1981. Paeoniflorigenone, a new monoterpene from Paeony root. Tetrahedron Lett 22: 3069-3070   DOI   ScienceOn
19 Kang, SS, Kim JS, Yun-Choi HS, Han BH. 1993. Phytochemical studies on Paeoniae radix. Kor J Pharmacogn 24: 247-250
20 Ro HS, Ko WK, Park KK, Cho YH, Park HS. 1997. Antihyperlipidemic effects of Bupleuri radix, Paeoniae radix and Uncariae ramulus et Uncus on experimental hyperlipidemia in rats. J Applied Pharm 5: 43-47
21 Ro HS, Ko WK, Yang HO, Park KK, Cho YH, Park HS. 1997. Effect of several solvent extracts from Paeoniae radix on experimental hyperlipidemia in rats. J Kor Pharm Sci 27: 145-151
22 Kaneda M, Iitakawa Y, Shibata S. 1972. Chemical studies on the oriental plant drugs.; Ⅹ. The absolute structures of paeoniflorin, albiflorin, oxypaeoniflorin and benzoylpaeoniflorin isolated from Chinese Paeony root. Tetrahedron 28: 4309- 4317   DOI   ScienceOn
23 Shibata S, Nakahara M, Aimi N. 1966. The occurrence of paeoniflorin in the plant of Paeonia spp. Shoyakugaku Zasshi 20: 37-39