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http://dx.doi.org/10.13103/JFHS.2022.37.5.328

Assessment of biofilm formation of Lacticaseibacillus rhamnosus LRH020  

Kim, Hye Rim (CH Labs Corp., Chong Kun Dang Health care Labs)
Seo, Eunsol (CH Labs Corp., Chong Kun Dang Health care Labs)
Seo, Min Yeong (ShinWoo Co., Ltd)
Kim, Byung-Yong (CH Labs Corp., Chong Kun Dang Health care Labs)
Publication Information
Journal of Food Hygiene and Safety / v.37, no.5, 2022 , pp. 328-331 More about this Journal
Abstract
Biofilms are complexly structured communities of microorganisms composed of surface-attached microorganisms, where their effects on the host have been controversial. In this study, we investigated the potential biofilm-forming capacity of Lacticaseibacillus rhamnosus LRH020 (DSM25568) by detecting genes known to promote biofilm formation. It was shown that the aggregation substance gene (asa 1) was presented in the LRH020 strain. Therefore, we investigated the phenotypic activities of the gene asa1 via two methods: biofilm formation and auto-aggregation activity. It was shown that the strain LRH020 had significantly less ability to form biofilm compared to the positive control strain Enterococcus faecalis ATCC 19433. Furthermore, LRH020 exhibited biofilm-forming activity comparable to Lacticaseibacillus rhamnosus GG (LGG), widely used probiotics. The auto-aggregation activity of LRH020 was also within the safe range similar to that of LGG. In conclusion, this study shows that both biofilm-forming and auto-aggregation activities of the LRH020 are comparable to one of the most studied probiotics strains, LGG.
Keywords
Probiotics; Lacticaseibacillus rhamnosus; Asa1; Biofilm; Auto-aggregation;
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