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http://dx.doi.org/10.13103/JFHS.2021.36.4.353

Antioxidant and Anti-Cholesterol Activities of Standardized Ecklonia Stolonifera Extract  

Han, Xionggao (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Kim, Woo-Hyeok (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Sun-Il (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Men, Xiao (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Lee, Se-jeong (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Jin, Heegu (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Oh, Hyun-Ji (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Kang, Dahye (Naturalway Co. Ltd)
Kim, HyungBin (Naturalway Co. Ltd)
Lee, Boo-Yong (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Lee, Ok-Hwan (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.36, no.4, 2021 , pp. 353-362 More about this Journal
Abstract
Ecklonia stolonifera, which belongs to the family Laminariaceae, is an edible perennial brown marine alga that is widely distributed, and is rich in polyphenols, including dieckol. Here, we investigated the radical scavenging activities of E. stolonifera extract (ESE) using various in vitro models. We further evaluated the effect of ESE on the cholesterol secretion inhibition activity in HepG2 cells, as well as the hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase activity. Our results showed that the total phenol, total flavonoid, and dieckol contents of ESE were 9.64±0.04 mg GAE/g, 2.72±0.08 mg RE/g and 27.42±0.66 mg/g, respectively. The antioxidant activity of ESE increased in a dose-dependent manner. In addition, the ESE inhibited cholesterol secretion from HepG2 cells with anti-HMG-CoA reductase activity. These results suggested that ESE possesses antioxidant and anti-cholesterol activities, and can therefore be used as a preclinical bioresource for development of health functional foods.
Keywords
Ecklonia stolonifera; Antioxidant activity; HMG-CoA reductase; Anti-cholesterol activity; Dieckol;
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1 Lee, S.J., Shin, J.H., Kang, M.J., Jung, W.J., Ryu, J.H., Kim, R.J., Sung, N.J., Antioxidants activity of aged red garlic. J. Life Sci., 20, 775-781 (2010).   DOI
2 Ou, B., Hampsch-Woodill, M., Prior, R.L., Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 49, 4619-4626 (2001).   DOI
3 Chae, K.S., Lee, S.J., Gim, S.W., Cho, S.W., Kwon, J.W., Kim, Y.S., Improvement in antioxidant activities and cholesterol secretion inhibition abilities of black raspberry extracts upon maturation. Korean J. Food Preserv., 27, 325-332 (2020).   DOI
4 Lachenmeier, D.W., Monakhova, Y.B., Kuballa, T., Lobell-Behrends, S., Maixner, S., Kohl-Himmelseher, M., Waldner, A., Steffen, C., NMR evaluation of total statin content and HMG-CoA reductase inhibition in red yeast rice (Monascus spp.) food supplements. Chin. Med., 7, 1-7 (2012).   DOI
5 Kim, H.J., Jun, B.S., Kim, S.K., Cha, J.Y., Cho, Y.S., Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L). J. Korean Soc. Food Sci. Nutr., 29, 1127-1132 (2000).
6 Son, H.J., Studies on the Antioxidant Activity and MetalChelating Ability of Seaweed by the Cultivating Locations. MS thesis, Kosin University, Busan, Korea (2007).
7 Kim, J.H., Kang, H.M., Lee, S.H., Lee, J.Y., Park, L.Y., Anti-oxidant and α-glucosidase inhibition activity of seaweed extracts. Korean J. Food Preserv., 22, 290-296 (2015).   DOI
8 Cerqueira, N.M.F.S.A., Oliveira, E.F., Gesto, D.S., Santos-Martins, D., Moreira, C., Moorthy, H.N., Ramos, M.J., Fernandes, P.A., Cholesterol biosynthesis: A mechanistic overview. Biochemistry, 55, 5483-5506 (2016).   DOI
9 Kuda, T., Kunii, T., Goto, H., Suzuki, T., Yano, T., Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula, Japan. Food Chem., 103, 900-905 (2007).   DOI
10 Sirtory, C.R., Pharmacology and mechanism of action of the new HMG CoA reductase inhibitors. Pharmcol. Res., 22, 555-563 (1990).   DOI
11 Brown, S.L., Lowered serum cholesterol and low mood. BMJ, 313, 637-638 (1996).   DOI
12 Shin, M.K., Han, S.H., Effects of methanol extracts from bamboo (P seudosasa uaponica Makino) leaves extracts on lipid metabolism in rats fed high fat and high cholesterol diet. J. Korean Soc. Food Cult., 17 30-36 (2002).
13 Kim, T.H., Son, Y.K., Hwang, K.H., Kim, M.H., Effects of Angelica keiskei Koidzumi and turmeric extract supplementation on serum lipid parameters in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. J. Korean Soc. Food Sci. Nutr., 37, 708-713 (2008).   DOI
14 Kim, M.K., Cho, S.W., Park, Y.K., Long-term vegetarians have low oxidative stress, body fat, and cholesterol levels. Nutr. Res. Pract., 6, 155-161 (2012).   DOI
15 Tyrovolas, S., Panagiotakos, D.B., The role of Mediterranean type of diet on the development of cancer and cardiovascular disease, in the elderly: A systematic review. Maturitas, 65, 122-130 (2010).   DOI
16 Yeo, A.R., Lee, J., Tae, I.H., Park, S.R., Cho, Y.H., Lee, B.H., Shin, H.C., Kim, S.H., Yoo, Y.C., Anti-hyperlipidemic effect of polyphenol extract (Seapolynol™) and dieckol isolated from Ecklonia cava in in vivo and in vitro models. Prev. Nutr. Food Sci., 17, 1-7 (2012).   DOI
17 Jo, H.G., Kim, M.J., Cheong, S.H., Sea tangle (Saccharina japonica), an edible brown seaweed, improves serum lipid profiles and antioxidant status in rats fed high-fat and high-cholesterol diets. J. Appl. Phycol., 31, 3957-3967 (2019).   DOI
18 Kang, H.S., Chung, H.Y., Kim, J.Y., Son, B.W., Jung, H.A., Choi, J.S., Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res. 27, 194-198 (2004).   DOI
19 Manandhar, B., Wagle, A., Seong, S.H., Paudel, P., Kim, H.R., Jung, H.A., Choi, J.S., Phlorotannins with potential anti-tyrosinase and antioxidant activity isolated from the marine seaweed ecklonia stolonifera. Antioxidants, 8, 240 (2019).   DOI
20 Goo, H.R., Choi, J.S., Na, D.H., Quantitative determination of major phlorotannins in ecklonia stolonifera. Arch. Pharm. Res., 33, 539-544 (2010).   DOI
21 Park, E.Y., Kim, E.H., Kim, M.H., Seo, Y.W., Lee, J.I., Jun, H.S., Polyphenol-rich fraction of brown alga Ecklonia cava collected from Gijang, Korea, reduces obesity and glucose levels in high-fat diet-induced obese mice. Evid. Based complement. Alternat. Med., 2012, 418912 (2012).
22 Yu, S.M., Kim, W.K., Health beneficial effects of brown algae ecklonia stolonifera in liver. Food Sci. Ind., 51, 334-342 (2018).   DOI
23 Duval, B., Shetty, K., The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J. Food Biochem., 25, 361-377 (2007).   DOI
24 Bang, C.Y., Byun, J.H., Choi, H.K., Choi, J.S., Choung, S.Y., Protective effects of Ecklonia stolonifera extract on ethanol-induced fatty liver in rats. Biomol. Ther., 24, 650-658 (2016).   DOI
25 Oyaizu, M., Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Jpn. J. Nutr., 44, 307-315 (1986).   DOI
26 Benzie, I.F., Stranin, J.J., The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal. Biochem., 239, 70-76 (1996).   DOI
27 National Institutes of Health (NIH). Lowering blood cholesterol to prevent heart disease. JAMA, 253, 2080-2086 (1985).   DOI
28 Qureshi, A.A., Abuirmeileh, N., Din, Z.Z., Elson, C.E., Burger, W.C., Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic. Lipids, 18, 343-348 (1983).   DOI
29 Buyukokuroglu, M.E., Gulcin, I., Oktay, M., Kufrevioglu, O.I., In vitro antioxidant properties of dantrolene sodium. Pharmacol. Res., 44, 491-494 (2001).   DOI
30 Lee, S.J., Park, J.Y., Nam, C.M., Jee, S.H., The prevalence estimation of metabolic syndrome and it's related factors based on data from general health medical examination: A multi-center study. J. Health Info. Stat., 33 119-134 (2008).
31 Ebrahim, S., Sung, J.H., Song, Y.M., Ferrer, R.L., Lawlor, D.A., Smith, G.D., Serum cholesterol, haemorrhagic stroke, ischaemic stroke, and myocardial infarction: Korean national health system prospective cohort study. BMJ, 333, 22-27 (2006).   DOI
32 Hallivell, B., Aeschbach, R., Loliger, J., Aruoma, O.I., The characterization of antioxidants. Food Chem. Toxicol., 33, 601-617 (1995).   DOI
33 Ozgen, M., Reese, R.N., Tulio Jr, A.Z., Scheerens, J.C., Miller A.R., Modified 2,2-azino-bis-3-ethylbenzothiazoline6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2' diphenyl-1-picrylhydrazyl (DPPH) methods. J. Agric. Food Chem. 54, 1151-1157 (2006).   DOI
34 Stranahan, A.M., Cutler, R.G., Button, C., Telljohann, R., Mattson, M.P., Diet-induced elevations in serum cholesterol are associated with alterations in hippocampal lipid metabolism and increased oxidative stress. J. Neurochem., 118, 611-615 (2011).   DOI
35 Choi, J.S., Han, Y.R., Byeon, J.S., Choung, S.Y., Sohn, H.S., Jung, H.A., Protective effect of fucosterol isolated from the edible brown algae, Ecklonia stolonifera and Eisenia bicyclis, on tert-butyl hydroperoxide- and tacrine-induced HepG2 cell injury. J. Pharm. Pharmacol., 67, 1170-1178 (2015).   DOI
36 Jin, H.G., Lee, K.P., Chei, S.W., Oh, H.J., Lee, K.P., Lee, B.Y., Ecklonia stolonifera Extract Suppresses Lipid Accumulation by Promoting Lipolysis and Adipose Browning in High-Fat Diet-Induced Obese Male Mice. Cells, 9, 871 (2020).   DOI