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http://dx.doi.org/10.13103/JFHS.2020.35.6.561

Development and Validation of Multiplex PCR Method for the Identification of Pagrus major and Sciaenops ocellatus  

Choi, Iseul (Department of Food Science and Technology, Pukyoung National University)
Shin, Jiyoung (Department of Food Science and Technology, Pukyoung National University)
Yang, Ji-young (Department of Food Science and Technology, Pukyoung National University)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.6, 2020 , pp. 561-566 More about this Journal
Abstract
Nowadays, with increase of seafood consumption, there have been increasing reports of defective seafood products. There have been incidents of red drum (Sciaenops ocellatus) being sold as red seabream (Pagrus major). In this study, we sought to develop and validate species-specific PCRs to differentiate between P. major and S. ocellatus to prevent the sale of S. ocellatus as P. major. Primers for P. major were designed to bind 12s rRNA and those for S. ocellatus were designed to bind 16s rRNA. Multiplex PCR showed a 468 bp amplicon for P. major and a 181 bp amplicon for S. ocellatus. The limit of detection of P. major and S. ocellatus was present at 1 ng each. The developed primers were validated with 19 P. major samples of food items purchased through the internet. Using this monitoring method, the experimental results and tested species were in agreement. Hence, the developed multiplex PCR method is considered reliable to authenticate P. major and S. ocellatus.
Keywords
Pagrus major; Sciaenops ocellatus; Multiplex PCR;
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Times Cited By KSCI : 15  (Citation Analysis)
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