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http://dx.doi.org/10.13103/JFHS.2020.35.4.334

Improvement of the Detection Technique of Listeria monocytogenes through Modification of the Enrichment Medium and DNA Extraction Buffer  

Lee, Jeeyeon (Department of Food & Nutrition, Dong-eui University)
Seo, Yeongeun (Department of Food and Nutrition, Sookmyung Women's University)
Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.4, 2020 , pp. 334-340 More about this Journal
Abstract
In this study we developed an enrichment medium and lysis buffers to detect Listeria monocytogenes in meat and processed meat products under various lysis conditions. The enrichment efficiency of L. monocytogenes medium listed in the Food Standards was compared, and thus, Listeria Enrichment Broth (LEB) was modified by adding supplements such as carbon source and minerals. The lysis buffers were developed to extract L. monocytogenes DNA quickly and efficiently under various lysis conditions. L. monocytogenes was most rapidly grown in LEB containing 0.1% pyruvate and 0.1% ferric citrate. A lysis buffer mixed with 0.5% or 1% N-lauroylasrcosine sodium salt, 0.5 N NaOH and 0.5 M EDTA for 30 min at room temperature was found to be the best in terms of DNA purity and yield. These results indicate that developed enrichment medium and lysis buffer can be used to detect L. monocytogenes in meat and processed meat products rapidly and efficiently.
Keywords
Listeria monocytogenes; Detection; Enrichment medium; DNA extraction buffer;
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1 Lee, J., Gwak, E., Lee, H., Ha, J., Lee, S., Kim, S., Oh, M.H., Park, B.Y., Choi, K.H., Yoon, Y., Effects of low $NaNO_2$ and NaCl concentrations on Listeria monocytogenes growth in emulsion-type sausage. Asian-Australasian J. Anim. Sci., 30, 432-438 (2017).   DOI
2 Liu, Y., Sun, W., Sun, T., Gorris, L.G., Wang, X., Liu, B., Dong, Q., The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach. Int. J. Food Microbiol., 312, 108358 (2020).   DOI
3 Rietberg, K., Lloyd, J., Melius, B., Wyman, P., Treadwell, R., Olson, G., Kang, M.G., Duchin, J.S., Outbreak of Listeria monocytogenes infections linked to a pasteurized ice cream product served to hospitalized patients. Epidemiol. Infect., 144, 2728-2731 (2016).   DOI
4 Karyotis, D., Skandamis, P.N., Juneja, V.K., Thermal inactivation of Listeria monocytogenes and Salmonella spp. in sous-vide processed marinated chicken breast. Food Res. Int., 100, 894-898 (2017).   DOI
5 Tangwatcharin, P., Sorapukdee, S., Kongsrirat, K., Sousvided restructured goat steaks: process optimized by thermal inactivation of Listeria monocytogenes and their quality characteristics. Food Sci. Anim. Resour., 39, 863-876 (2019).   DOI
6 Thangavel, G., Subramaniyam, T., Antimicrobial efficacy of Leuconostoc spp. isolated from Indian meat against Escherichia coli and Listeria monocytogenes in Spinach leaves. Food Sci. Anim. Resour., 39, 677-685 (2019).   DOI
7 Ministry of Food and Drug Safety, (2020, July 14). Food standards. Retrieved from http://www.foodsafetykorea.go.kr/foodcode/index.jsp
8 Lee, Y., Yoon, Y., Seo, Y., Kim, S., Ha, J., Lee, J., Choi, Y., Oh, H., Kim, Y., Kang, J., Park, E., Kim, W.I., Lee, S., Combined enrichment and quantitative polymerase chain reaction to improve sensitivity and reduce time of detection of Listeria monocytogenes in mushrooms. Foodborne Pathog. Dis., 17, 276-283 (2020).   DOI
9 Delibato, E., Gattuso, A., Minucci, A., Auricchio, B., De Medici, D., Toti, L., Castangnola, M., Cpoluongo, E., Gianfranceschi, M.V., PCR experion automated electrophoresis system to detect Listeria monocytogenes in foods. J. Sep. Sci., 32, 3817-3821 (2009).   DOI
10 Furrer, B., Candrian, U., Hoefelein, C., Luethy, J., Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. J. Appl. Bacteriol., 70, 372-379 (1991).   DOI
11 Silk, T.M., Roth, T.M., Donnelly, C.W., Comparison of growth kinetics for healthy and heat-injured Listeria monocytogenes in eight enrichment broths. J. Food Prot., 65, 1333-1337 (2002).   DOI
12 McLaughlin, H.P., Hill, C., Gahan, C.G., The impact of iron on Listeria monocytogenes; inside and outside the host. Curr. Opin. Biotechnol., 22, 194-199 (2011).   DOI
13 Clark, M.S., 1997. Plant Molecular Biology - A Laboratory Manual. Springer, Berlin/ Heidelberg, Germany, pp.305-328.
14 Popa, O.P., Murariu, D.U.M.I.T.R.U., Popa, L.O., Comparison of four DNA extraction methods from invasive freshwater bivalve species (Mollusca: Bivalvia) in Romanian fauna. Trav. Mus. Natl. Hist. Nat. Grigore Antipa, 6, 527-536 (2007).
15 Poms, R., Glössl, J., Foissy, H., Increased sensitivity for detection of specific target DNA in milk by concentration in milk fat. Eur. Food Res. Technol., 213, 361-365 (2001).   DOI
16 Thermo Fisher Scientific, (2020, August 11). PCR setup-Six Critical Components to Consider. Retrieved from https://www.thermofisher.com/kr/ko/home/life-science/cloning/cloninglearning-center/invitrogen-school-of-molecular-biology/pcr-education/pcr-reagents-enzymes/pcr-component-considerations.html
17 Chen, Y., Chen, M., Wang, J., Wu, Q., Cheng, J., Zhang, J., Sun, Q., Xue, L., Zeng, H., Lei, T., Pang, R., Ye, Q., Wu, S., Zhang, S., Wu, H., Li, W., Kou, X., Heterogeneity, characteristics, and public health implications of Listeria monocytogenes in ready-to-eat foods and pasteurized milk in China. Front. Microbiol., 11, 642 (2020).   DOI
18 Demaitre, N., Van Damme, I., De Zutter, L., Geeraerd, A.H., Rasschaert, G., De Reu, K., Occurrence, distribution and diversity of Listeria monocytogenes contamination on beef and pig carcasses after slaughter. Meat Sci., 108177 (2020).