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http://dx.doi.org/10.13103/JFHS.2020.35.4.312

Determination of Neonicotinoid Pesticides in Commercial Agricultural Products by LC-MS/MS  

Hwang, Lae-hong (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Yang, Hye-ran (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Jae-kyoo (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Chang-kyu (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Min-jung (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.4, 2020 , pp. 312-318 More about this Journal
Abstract
A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for neonicotinoid pesticide analysis in agricultural products. Four compounds (imidacloprid, clothianidin, acetamiprid, thiacloprid) were extracted with acetonitrile from agricultural products and cleaned up by NH2 solid-phase extraction procedure, and eluted with 0.1% formic acid in methanol/dichloromethane (5/95, v/v). The limit of detection and quantification were 0.0001-0.0005 mg/kg and 0.001 mg/kg, respectively. The mean recoveries of neonicotinoid pesticide from agricultural products were in the range of 90.7-100.9% and 94.4-99.8%, as spiked at 0.2 mg/kg and 0.02 mg/kg, respectively. This validation satisfied the national criteria for pesticide analytical methods. In summary, The present method is fast, precise and sensitive enough for the Positive List System (PLS), and we conclude that the method is also suitable for neonicotinoid pesticide determination in a wide range of agricultural products.
Keywords
Neonicotinoid Pesticide; LC-MS/MS; Agricultural product;
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1 Kim, E.H., Lee, J.H., Sung, J.H., Lee, J.H., Shin, Y.H., Kim, J.H., Exposure and risk assessment for operator exposure to insecticide acetamiprid during water melon cultivation in greenhouse using whole body dosimetry, Korean J. Pestic. Sci., 18(4), 247-257 (2014).   DOI
2 Korea Rural Economic Institute, (2020, January 20), Insecticide "Neonicotinoid" banned in EU, Retrieved from https://www.krei.re.kr/wldagr/selectBbsNttView.do?key=162&bbsNo=66&nttNo=45049
3 Korea Agro-Fisheries & Food Trade Corporation, (2020, January 20), Insecticide "Neonicotinoid" banned in EU, Retrieved from https://www.kati.net/board/exportNewsView.do?board_seq=86035
4 Hwang, L.H., Lee, S.D., Kim, J.G., Kim, J.Y., Park, S.H., Kim, J.H., Park, J.H., Han, C.H., Kim, M.S., Determination of carbendazim in commercial agricultural products by LCMS/MS. J. Food Hyg. Saf., 32(2), 1-6 (2017).   DOI
5 Lee, Y.D., 2012. Handbook for the pesticide residue analytical methods of food code, National Institution of Food and Drug Safety Evaluation, Cheongwon, pp. 78-82.
6 Ministry of Food and Drug Safety : Korea food code, https://www.mfds.go.kr., 235-380 (2015).
7 Kim, J.Y., Jung, Y.M., Oh, H.S., Kang, S.T., Monitoring and risk assessment of pesticide residues in commercial environment- friendly agricultural products distributed using LCMS/ MS in seoul metropolitan area. Korean J. Food Sci. Technol., 47(3), 306-320 (2015).   DOI
8 Manuel, M.S., Mercedes, M., F.Javier A., Jose, L.M.V., Analysis of acetamiprid in vegetables using gas chromatography-tandem mass spectrometry, Anal. Sci., 19, 701-704 (2003).   DOI
9 Kim, N.K., Lee, S.H., Nam, Y.J., Moon, K.M., Park, M.H., Yun, M.H., Kim, M.Y., Jang, H.M., Shin, B.S., Monitoring of neonicotinoid pesticide residues in paprika using UPLCMS/MS from Gyeongnam Region. Korean J. Pestic. Sci., 15(1), 15-21 (2011).
10 Park, B.J., Son, K.A., Paik, M.K., Kim, J.B., Hong, S.M., Im, G.J., Hong, M.K., Monitoring of neonicotinoid pesticide residues in fruit vegetable and human exposure assessment. Korean J. Pestic. Sci., 14(2), 104-109 (2010).
11 Bai, D., Lummis, S., Leicht, W., Breer, H., Sattelle, D., Actions of imidacloprid and a related nitromethylene on cholineric receptors of an identified insect motor neurone. Pestic. Sci., 33(2), 197-204 (1991).   DOI
12 Matsuda, K., Buckingham, S., Kleier, D., Rauh, J., Grauso, M., Sattelle, D., Neonicotinoids ; insecticides action on insect nicotinic acetylcholine receptor. Trends Pharmacol. Sci., 22(11), 573-580 (2001).   DOI
13 Zhang, A., Kaiser, H., Maienfisch, P., Casida, J., Insect nicotinic acetylcholine receptor ; conserved neonicotinoid specificity of (3H) imidacloprid binding site. J. Neurochem., 75(3), 1294-1303 (2000).   DOI
14 Lee, E.Y., Noh, H.H., Park, Y.S., Kang, K.W., Lee, K.H., Park, H.K., Yun, S.S., Jin, C.W., Han, S.K., Kyung, K.S., Residual characteristics of neonicotinoid insecticide dinotefuran and thiacloprid in cucumber, Korean J. Pestic. Sci., 13(2), 96-104 (2009).
15 Kim, J.W., Lee, J.M., Lee, D.S., Kang, S.T., Kim, D.W., Lee, D.S., Riu, K.Z., Boo, K.H., Residual chracteristics of insecticide acetamiprid in asparagus under greenhouse condition. Korean J. Pestic. Sci., 19(3), 204-209 (2015).   DOI