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http://dx.doi.org/10.13103/JFHS.2018.33.5.412

Quantitative Cell Count of Vibrio vulnificus Cells Based on MPN-PCR Method  

Jang, Yu-Mi (Department of Food Science and Technology, Pukyong National University)
Park, Seul-Ki (Department of Food Science and Technology, Pukyong National University)
Jeong, Hee-Jin (Department of Food Science and Technology, Pukyong National University)
Lee, Jang-Won (Department of Food Science and Technology, Pukyong National University)
Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)
Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
Shin, Il-Shik (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.5, 2018 , pp. 412-415 More about this Journal
Abstract
The objective of this study was to establish a quantitative count method of Vibrio vulnificus cells. Plate count method is often used to count the number of V. vulnificus cells using thiosulfate citrate bile salts sucrose (TCBS) agar plate. However, this method is unsuitable for counting V. vulnificus cells due to growth inhibition and cell injuries in TCBS medium. In this study, we suggested a most probable number-polymerase chain reaction (MPN-PCR) method using alkaline peptone water medium for the quantification of V. vulnificus. This MPN-PCR method showed 2 log higher cell number than TCBS agar plate method. Similar results were also found in the control using, Luria-Bertani agar containing 2% NaCl. Thus, this MPN-PCR method can be used a sensitive method for quantitative count of viable V. vulnificus cells in fish and shellfish samples.
Keywords
Injured cell; MPN-PCR; Quantitative cell count; Vibrio vulnificus;
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