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http://dx.doi.org/10.13103/JFHS.2018.33.5.354

Method Development for Determination of Trichothecene Mycotoxins in Nuts by LC-MS/MS  

Kim, Dan-Bi (Korea Food Research Institute)
Park, Ji-Su (Korea Food Research Institute)
Yoo, Mi-Young (Korea Food Research Institute)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.5, 2018 , pp. 354-360 More about this Journal
Abstract
This study presents a method validation for extraction and quantitative analysis of trichothecene mycotoxins in nuts based on quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for extraction and enhanced matrix removal (EMR)-lipid-disperive-SPE (d-SPE) cleanup method, with detection and quantification by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in positive- and negative-ion modes. Linearity, precision, and accuracy were validated for LC-MS/MS methods. Results obtained with LC-MS/MS were linear, with correlation coefficient ($R^2$) of 0.998. Limits of detection and quantification for mycotoxins were $0.41-3.57{\mu}g/kg$ and $1.23-10.82{\mu}g/kg$, respectively. Intra- and inter-day precisions (RSD, %) were 0.40-8.44% and 1.93-12.46%, respectively. Results indicated to be rapidly and accurately identifying trichothecene mycotoxins and may be used as a suitable safety management method for nuts and nuts related commodities.
Keywords
Trichothecene mycotoxins; Nuts; QuEChERS; LC-MS/MS; Analytical method;
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