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http://dx.doi.org/10.13103/JFHS.2018.33.4.289

Development and Validation of Analytical Method for Wogonin, Quercetin, and Quercetin-3-O-glucuronide in Extracts of Nelumbo nucifera, Morus alba L., and Raphanus sativus Mixture  

Jang, Gill-Woong (Department of Food Science and Biotechnology, Kangwon National University)
Park, Eun-Young (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Sun-il (Department of Food Science and Biotechnology, Kangwon National University)
Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University)
Sim, Wan-Sup (Department of Food Science and Biotechnology, Kangwon National University)
Han, Xinggao (Department of Food Science and Biotechnology, Kangwon National University)
Cho, Hyun-Duk (Haram Co. Ltd.)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.4, 2018 , pp. 289-295 More about this Journal
Abstract
The aim of this study was to develop and validate an analytical method for determining the presence of wogonin, quercetin, and quercetin-3-O-glucuronide in extracts of Nelumbo nucifera, Morus alba L., and Raphanus sativus mixtures. We evaluated the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of analytical methods for wogonin, quercetin, and quercetin-3-O-glucuronide using high performance liquid chromatography. Our result showed that the correlation coefficients of the calibration curve for wogonin, quercetin, and quercetin-3-O-glucuronide were 0.9999. The LOD for wogonin, quercetin, and quercetin-3-O-glucuronide ranged from 0.09 to 0.16 and those for the LOQ ranged from 0.26 to $0.48{\mu}g/mL$. The inter-day and intra-day precision values of wogonin, quercetin, and quercetin-3-O-glucuronide ranged from 0.74 to 1.87 and from 0.28 to 1.12%, respectively. The inter-day and intra-day accuracies were 99.96~115.88% and 99.73~114.81%, respectively. Therefore, the analytical method was validated for the detection of wogonin, quercetin, and quercetin-3-O-glucuronide in extracts of Nelumbo nucifera, Morus alba L., and Raphanus sativus mixtures.
Keywords
Wogonin; Quercetin; Quercetin-3-O-glucuronide; HPLC; Method validation;
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