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http://dx.doi.org/10.13103/JFHS.2018.33.4.266

Optimization of Analytical Methods for Octacosanol in Related Health-functional Foods with GC-MS  

Lee, Jin Hee (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Oh, Mi Hyune (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Lee, Kyung Jin (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Kim, Yang Sun (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Keum, Eun Hee (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Park, Ji Eun (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Cho, Mee Hyun (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Seong, Min Hye (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Kim, Sang A (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Kim, Mee hye (Imported Food Analysis Division, Gyeongin Regional Office of Food and Drug Safety, Ministry of Food and Drug Safety)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.4, 2018 , pp. 266-271 More about this Journal
Abstract
The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health-functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we continue our research on developing analytical methods for the items. Octacosanol is the major component of polycosanol and is a high-molecular-mass primary fatty alcohol, obtained from sugar cane wax. Previous researchers have shown that octacosanol can lower cholesterol and has antiaggregatory properties, cytoprotective uses, and ergogenic properties for human health. Recently, octacosanol products have been actively introduced into the domestic market because of their functional biological activity. We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographic-tandem mass spectrometry (GC-MS). The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of $12.5{\sim}200{\mu}g/L$. Standard calibration curves presented linearity with the correlation coefficient ($r^2$) > 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were $4.5{\mu}g/L$ and $13.8{\mu}g/L$, respectively. The high recoveries (92.5 to 108.8%) and precision (1.8 to 2.4%) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.
Keywords
Octacosanol; GC-MS; Dietary supplement; Method validation;
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1 Kazuko, K., Kumlko, A. Yohel, H.: Free primary alcohols in oils and waxes from germs, kernels and other components of nut, seeds, fruits and cereals. J. Am. Oil Chem. Soc. 68(11), 869 (1991).   DOI
2 Cureton M et al., Maximal voluntary and functional performance levels needed for independence in adults aged 65 to 97 years. Phys. Ther., 83(1), 37-48 (1972).
3 Kabir Y et al., Tonsue distribution of octacosanol in liver and muscle of rats after serial administration. Ann. Nutr. Metab., 39(5), 279-284 (1995).   DOI
4 Cureton, T.K.: The physiological effects of wheat germ oil on human in exercise, Charles C. Thomas Publisher (1972).
5 Kato, S., Karino, K., Hasegawa, J., Nagasaki, A., Eguchi, M. and Ichinose, T.: Octacosanol affects lipid metabolism in rats fed on a high fat diet. British J. Nutr. 73, 433 (1995).   DOI
6 Health Fuctional Food Code, 100-101, Ministry of Food and Drug Safety (MFDS) Notice 2016-143 (2016).
7 2017 Food & Drug Statistical Yearbook, 41, Ministry of Food and Drug Safety (MFDS) (2017).
8 Daniela H, Miriam B, Alfonso V, Luis A. V, Trace quantification of 1-octacosanol and 1-triacontanol and their main metabolites in plasma by liquid-liquid extraction coupled with gas chromatography-mass spectrometry. Chromatog. B., 877, 4154-4158 (2009).   DOI
9 Anna L, Romina B, Nicolas S, Tal I, Jan E L, Hanjo H, Jocelyn KC R, Alisdair R F, Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues. Plant Methods. 5(1), 4 (2009).   DOI
10 Han Y, Chunmin W, Haibo S, Ruichen G, Quality Evaluation of Policosanol film-coated tablets by Simultaneous determination of Eight Ingredients Using GC-FID and GC-MS. J. Anal. Sci. Methods and Inst., 2, 24-28 (2012).
11 David M. D, Luis G. B, Trace determination of 1-octacosanol in rat plasma by solid-phase extraction with Tenax GC and capillary gas chromatography. J. Chromatography B. 762, 43-49 (2001).   DOI
12 Shiyi O, Jian Z, Yong W, Ye T, Jiong W, Prepartion of octacosanol from filter mud produced after sugarcane juice clarification. LWT- Food Sci. Technol., 45, 295-298 (2012).   DOI
13 Simin F, Zisheng L, Fangfang Z, Songbai L, Zia U. K, Effect of water, metallic ions, fatty acid and temperature on oxidative stability of 1-octacosanol from sugarcane rind. Food Chem., 182, 171-177 (2015).   DOI
14 Sirin, S., Siwarutt B, Suwatchai J, Issara S, Wichchunee P, Kittiwut K, Purification of policosanol from crude sugarcane wax obtained from supercritical $CO_2$ extraction. The 6th Internationcal conference on Natural Products for Health and Beauty (NATPRO6). 29-33 (2016).