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http://dx.doi.org/10.13103/JFHS.2018.33.1.58

Analytical Method for Determination of Cephalexin in Bovine Edible Tissues using Liquid Chromatography Coupled to Tandem Mass Spectrometry  

Chae, Won-Seok (Department of Chemistry, Daejin University)
Lee, Sung Joong (Department of Chemistry and Research Institute of Live Science, Gyeongsang National University)
Son, Song-Ee (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Kim, Suk (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu-Jang (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.1, 2018 , pp. 58-64 More about this Journal
Abstract
An analytical method for the determination of cephalexin (CEX) in bovine tissues (muscle, liver, kidney and fat tissues) was developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Tissue samples were extracted by the liquid-liquid extraction based on 5% trichloroacetic acid (TCA). The chromatographic separation was achieved on a reverse phase $C_{18}$ column with gradient elution using a mobile phase of 20 mM hexafluroacetylacetone (HFAC)/50% acetonitrile (40:60). The procedure was validated according to the Ministry of Food and Drug Safety guideline determining accuracy, precision, and limit of detection. Mean recoveries of CEX from spiked edible tissues ($6{\sim}1,500{\mu}g/kg$) were 83.9~106.8%, and the relative standard deviation was between 2.3 and 14.8%. Linearities were obtained with the correlation coefficient ($r^2$) of > 0.999. Limit of detection and limit of quantification for the investigated CEX were 2~10 and $6{\sim}30{\mu}g/kg$, respectively. This method was reliable, sensitive, economical and suitable for routine monitoring of CEX residues in bovine edible tissues.
Keywords
Cephalexin; Bovine edible tissues; LC-MS/MS;
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