[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.13103/JFHS.2017.32.1.64

Combined Effect of Cold Plasma and UV-C Against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on Fresh-cut Lettuce

Seong, Ji-Yeong (Department of Food and Nutrition, Kookmin University)
Park, Mi-Jung (Department of Food and Nutrition, Kookmin University)
Kwon, Ki-Hyun (Korea Food Research Institute)
Oh, Se-Wook (Department of Food and Nutrition, Kookmin University)

Publication Information

Journal of Food Hygiene and Safety / v.32, no.1, 2017 , pp. 64-69 More about this Journal

Abstract

This study was conducted to investigate the effect of cold plasma combined with UV-C irradiation against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes on lettuce. E. coli O157:H7, S. Typhimurium, and L. monocytogenes, corresponding to approximately 5.82, 5.09, 5.65 log CFU/g, were inoculated on lettuce, respectively. Then, the lettuce was treated with cold plasma, UV-C and combination (cold plasma + UV-C), respectively. The treated lettuce was stored for 9 days at $4^{\circ}C$ for microbiological analysis and sensory evaluation. Cold plasma reduced the populations of E. coli O157:H7, S. Typhimurium, and L. monocytogenes by 0.26, 0.65, and 0.93 log CFU/g, respectively. Each microorganism were reduced by 0.87, 0.88, and 1.14 log CFU/g after UV-C treatment. And, the combined treatment that was treated by cold plasma after UV-C treatment reduced the populations of inoculated microorganisms by 1.44, 2.70, 1.62 log CFU/g, respectively. The all treatment significantly (p < 0.05) reduced the populations of all inoculated bacteria compared to untreated lettuce. UV-C combined with cold plasma was the most effective for reducing the pathogenic bacteria on lettuce, by showing log-reductions of ${\geq}2.0\;log\;CFU/g$ . All treatment was not significantly different until 6 day storage compared to control group in terms of appearance, texture and overall acceptability. Therefore, the combined treatment will be an effective intervention method to control the bacteria on lettuce.

Keywords

fresh-cut lettuce; cold plasma; UV-C irradiation; pathogens; reduction;

Citations & Related Records

Reference

1	Harris L, Farber J, Beuchat L, Parish M, Suslow T, et al.: Outbreaks associated with fresh produce: incidence, growth, and survival of pathogens in fresh and fresh-cut produce. Compr. Rev. Food. Sci. F. 2, 78-141 (2003). DOI
2	CDC (Centers for Disease Control and Prevention): Available from: http://wwwn.cdc.gov/foodborneoutbreaks/Default.aspx. Accessed on Nov. 08, 2013 (2013).
3	Sagong H-G, Lee S-Y, Chang P-S, Heu S, Ryu S, et al.: Combined effect of ultrasound and organic acids to reduce Escherichia coli O157: H7, Salmonella Typhimurium, and Listeria monocytogenes on organic fresh lettuce. Int. J. Food Microbiol. 145, 287-292 (2011). DOI
4	Zhang S, Farber J: The effects of various disinfectants against Listeria monocytogenes on fresh-cut vegetables. Food Microbiol. 13, 311-321 (1996). DOI
5	Beuchat L, Nail B, Adler B, Clavero M: Efficacy of spray application of chlorinated water in killing pathogenic bacteria on raw apples, tomatoes, and lettuce. J. Food Protect. 61, 1305-1311 (1998). DOI
6	Gelinas P, Goulet J: Neutralization of the activity of eight disinfectants by organic matter. J. Appl. Bacteriol. 54, 243-247 (1983). DOI
7	Kim J, Marshall MR, Du W-X, Otwell WS, Wei C-I: Determination of chlorate and chlorite and mutagenicity of seafood treated with aqueous chlorine dioxide. J. Agr. Food Chem. 47, 3586-3591 (1999). DOI
8	Feng H, Sun P, Chai Y, Tong G, Zhang J, et al.: The interaction of a direct-current cold atmospheric-pressure air plasma with bacteria. IEEE T. Plasma Sci. 37, 121-127 (2009). DOI
9	Koban I, Matthes R, Hubner N-O, Welk A, Meisel P, et al.: Treatment of Candida albicans biofilms with low-temperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jet. New J. Phys. 12, 073039 (2010). DOI
10	Bai N, Sun P, Zhou H, Wu H, Wang R, et al.: Inactivation of Staphylococcus aureus in water by a cold, He/ $O_2$ atmospheric pressure plasma microjet. Plasma Processes Polym. 8, 424-431 (2011). DOI
11	Escalona VH, Aguayo E, Martinez-Hernandez GB, Artes F: UV-C doses to reduce pathogen and spoilage bacterial growth in vitro and in baby spinach. Postharvest Biol. Tec. 56, 223-231 (2010). DOI
12	Sizer C, Balasubramaniam V: New intervention processes for minimally processed juices. Food Technol. 53, 64-67 (1999).
13	Park M-H, Kim J-G: Low-dose UV-C irradiation reduces the microbial population and preserves antioxidant levels in peeled garlic (Allium sativum L.) during storage. Postharvest Biol. Tec. 100, 109-112 (2015). DOI
14	Martinez-Hernandez GB, Huertas J-P, Navarro-Rico J, Gomez PA, Artes F, et al.: Inactivation kinetics of foodborne pathogens by UV-C radiation and its subsequent growth in fresh-cut kailan-hybrid broccoli. Food microbiol. 46, 263-271 (2015). DOI
15	Pasquali, Frederique, et al.: Atmospheric cold plasma process for vegetable leaf decontamination: A feasibility study on radicchio (red chicory, Cichorium intybus L.). Food Control 60, 552-559 (2016). DOI
16	Allende A, McEvoy JL, Luo Y, Artes F, Wang CY: Effectiveness of two-sided UV-C treatments in inhibiting natural microflora and extending the shelf-life of minimally processed 'Red Oak Leaf' lettuce. Food Microbiol. 23, 241-249 (2006). DOI
17	Lee K-H, Kim H-J, Woo KS, Jo C, Kim J-K, et al.: Evaluation of cold plasma treatments for improved microbial and physicochemical qualities of brown rice. LWT-Food Sci. Technol. 73, 442-447 (2016). DOI
18	Cheon H-L, Shin J-Y, Park K-H, Chung M-S, Kang D-H: Inactivation of foodborne pathogens in powdered red pepper (Capsicum annuum L.) using combined UV-C irradiation and mild heat treatment. Food Control 50, 441-445 (2015). DOI
19	Lim W, Harrison MA: Effectiveness of UV light as a means to reduce Salmonella contamination on tomatoes and food contact surfaces. Food Control 66, 166-173 (2016). DOI
20	Lavelli, Vera: Antioxidant activity of minimally processed red chicory (Cichorium intybus L.) evaluated in xanthine oxidase-, myeloperoxidase-, and diaphorase-catalyzed reactions. J. Agr. Food Chem. 56.16: 7194-7200 (2008). DOI
21	Reardon T, Timmer CP, Barrett CB, Berdegue J: The rise of supermarkets in Africa, Asia, and Latin America. Am. J. Agr. Econ. 85, 1140-1146 (2003). DOI
22	Dolan C, Humphrey J: Governance and trade in fresh vegetables: the impact of UK supermarkets on the African horticulture industry. J. Dev. Stud. 37, 147-176 (2000). DOI