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http://dx.doi.org/10.13103/JFHS.2017.32.1.50

Prevalence and Toxin Characteristics of Bacillus cereus Isolated from Drinking Cups in Spring  

Jo, Ah-Hyeon (Department of Food Science and Technology, SunChon National University)
Choi, Ha-Na (Department of Food Science and Technology, SunChon National University)
Heo, Dan-Bi (Department of Food Science and Technology, SunChon National University)
Kwon, Sun-Mok (Department of Food Science and Technology, SunChon National University)
Kim, Jung-Beom (Department of Food Science and Technology, SunChon National University)
Publication Information
Journal of Food Hygiene and Safety / v.32, no.1, 2017 , pp. 50-56 More about this Journal
Abstract
The purpose of this study was to investigate the microbiological contamination of water and drinking cups in springs and to estimate the toxin gene, enterotoxin production ability and antibiotic susceptibility of foodborne pathogens. Ten spring water and 34 drinking cups were tested. The average number of total aerobic bacteria and coliform bacteria in spring water were 1.8 log CFU/mL and 1.2 log CFU/mL, and in drinking cups were $4.7log\;CFU/100cm^2$ and $1.7log\;CFU/100cm^2$. Salmonella spp., Staphylococcus aureus, E. coli O157:H7, Listeria monocytogenes, Vibrio parahaemolyticus, Yersinia enterocolitica were not isolated from all of samples but Bacillus cereus was detected in 5 (14.7%) of 34 drinking cups. The nheA and entFM genes were major enterotoxin genes in B. cereus isolated from drinking cups. All of B. cereus tested in this study produce non-heamolytic enterotoxin but only 2 isolates possessed heamolysin BL enterotoxin producing ability. B. cereus was resistant to ${\beta}-lactam$ antibiotics. These results revealed that the sanitary conditions of drinking cups in spring should be improved promptly. The substitution carrying a personal drinking cup for the public drinking cups equipped in springs is suggested to prevent food-borne illness.
Keywords
spring water; drinking cup; Bacillus cereus; contamination;
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Times Cited By KSCI : 4  (Citation Analysis)
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