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http://dx.doi.org/10.13103/JFHS.2017.32.1.20

Investigation of Microbiological Safety of on-farm Produce in Korea  

Kim, Won-Il (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Gwak, Min-Gyu (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Jo, A-Ra (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Ryu, Sang Don (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Se-Ri (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Ryu, Song Hee (Chemical Safety Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Hwang-Yong (Division for KOPIA (Korea Program on International Agriculture), Technology Cooperation Bureau, Rural Development Administration)
Ryu, Jae-Gee (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Food Hygiene and Safety / v.32, no.1, 2017 , pp. 20-26 More about this Journal
Abstract
Foodborne disease outbreaks associated with produces have been increasing in occurrence worldwide. This study investigated microbial contamination levels on thirteen kinds of agricultural products from farms stage to evaluate potential hazards associated with foodborne illness. A total of 1,820 samples were collected in major cultivating area from 2013 through 2015, and analyzed to enumerate aerobic bacterial counts, coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study for four kinds of microorganisms (Escherichia coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.01 to 7.18 log CFU/g, with the highest bacterial cell counts recorded for watermelon. Coliforms were detected in 651 samples (35.8%) with a minimum of 0.01 log CFU/g and a maximum of more than 5 log CFU/g. B. cereus was detected in 169 samples (9.3%) ranging from < 0.01 to 2.48 log CFU/g among total samples analyzed. S. aureus was detected in 14 samples (0.7%) with a minimum of 0.01 log CFU/g and a maximum of 1.69 log CFU/g. E. coli was detected in 101 samples (5.5%) among 1,820 samples. E. coli O157:H7, Salmonella spp. and L. monocytogenes were not detected in any of the samples. The microbial contamination levels of several agricultural products determined in this study may be used as the fundamental data for microbiological risk assessment (MRA).
Keywords
foodborne pathogen; microbial contamination; produce;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
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1 Koseki, S. and Isobe, S.: Prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. Int J Food Microbiol, 104, 239-248 (2005).   DOI
2 Won, Y.J., Yoon, C.Y., Seo, I.W., Nam, H.S., Lee, D.M., Park, D.H., Lee, H.M., Kim, S.S. and Lee, K.Y.: The study for the occurrence of food poisoning bacteria in organic vegetables, Kwangju regional FDA, pp. 99-117 (2002).
3 Choi, J.W., Park, S.Y., Yeon, J.H., Lee, M.J., Chung, D.H., Lee, K.H., Kim, M.G., Lee, D.H., Kim, K.S. and Ha S.D.: Microbial contamiantion levels of fresh vegetables distributed in markets. J. Fd Hyg. Safety, 20, 43-47 (2005).
4 Hong, C.K., Seo, Y.H., Choi, C.M., Hwang, I.S. and Kim, M.S.: Microbial quality of fresh vegetables and fruits in Seoul, Korea. J. Fd Hyg. Safety, 27, 24-29 (2012).   DOI
5 Kim, W.I., Jung, H.M., Kim, S.R., Park, K.H., Kim, B.S., Yun, J.C. and Ryu, K.Y.: Investigation of microbial contamination levels of leafy greens and its distributing conditions at different time-focused on perilla leaf and lettuce-. J. Fd Hyg. Safety, 27, 277-284 (2012).   DOI
6 Kim, W.I., Jo, A.R., Lee, J.H., Kim, S.R., Park, K.H., Nam, K.W., Yoon, Y., Yoon, D.H., Oh, S.Y., Lee, M.H., Ryu, J.G. and Kim, H.Y.: Survey of microbial contamination of tomatoes at farms in Korea. J. Fd Hyg. Safety, 28, 324-329 (2013).   DOI
7 Kim, J.W., Yu, Y.M., Na, W.S., Baljii, E., Choi, I.W., Youn, Y.N. and Lee, Y.H.: Monitoring of biosafety of agricultural products from urban community gardens and roof gardens in Korea. Kor. J. Hort. Sci. Technol., 32, 400-407 (2014).
8 Forsythe, S.J.: The microbiology of safe food, 2nd edition, Wiley-Blackwell (2010).
9 Johnston, L.M., Jaykus, L.A., Moll, D., Martinez, M.C., Anciso, J., Mora, B. and Moe, C.L.: A field study of the microbiological quality of fresh produce. J Food Prot, 68, 1840-1847 (2005).   DOI
10 Kim, J.H., Lee, Y.K. and Yang, J.Y.: Analysis on hazard microorganisms in raw materials and processing environment for Sunsik manufacture. J. Fd Hyg. Safety, 26, 410-416 (2011).
11 Nam, M.J., Heo, R.W., Lee, W.G., Kim, K.Y., Chung, D.Y., Kim, J.S., Shim, W.B. and Chung, D.H.: Microbiological hazard analysis of hot pepper farms for the application of Good Agricultural Practices (GAP) system. Journal of Agriculture & Life Science, 45, 163-173 (2012).
12 Choi, N.J., Bahk, G.J., Ha, S.D., Chung, M.S., Lee, S.H., Hwang, I.G., Park, J.H., Kim, G.H. and Oh, D.H.: Analysis of microbiological contamination levels of cabbage and freshcut produce on difference area toward climate in Korea. J. Fd Hyg. Safety, 27, 209-214 (2012).   DOI
13 Beuchat, L.R. and Ryu, J.H.: Produce handling and processing practices. Emerg Infect Dis, 3, 459-465 (1997).   DOI
14 Beuchat, L.R.: Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes and infection / Institut Pasteur, 4, 413-423 (2002).   DOI
15 Islam, M., Doyle, M.P., Phatak, S.C., Millner, P. and Jiang, X.: Persistence of enterohemorrhagic Escherichia coli O157: H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water. J Food Prot, 67, 1365-1370 (2004).   DOI
16 Niemira, B.A. and Cooke, P.H.: Escherichia coli O157:H7 biofilm formation on Romaine lettuce and spinach leaf surfaces reduces efficacy of irradiation and sodium hypochlorite washes. J Food Sci, 75, M270-277 (2010).   DOI
17 Allen, K.J., Kovacevic, J., Cancarevic, A., Wood, J., Xu, J., Gill, B., Allen, J.K. and Mesak, L.R.: Microbiological survey of imported produce available at retail across Canada. Int J Food Microbiol, 162, 135-142 (2013).   DOI
18 U.S. Food and Drug Administration (FDA): Bad Bug Book, Foodborne pathogenic microorganisms and natural toxins. 2nd edition, pp. 9-100 (2012).
19 Ölmez, H. and Temur, S.D.: Effects of different sanitizing treatments on biofilms and attachment of Escherichia coli and Listeria monocytogenes on green leaf lettuce. LWT - Food Science and Technology, 43, 964-970 (2010).   DOI
20 Scharff, R.L.: Health-related costs from foodborne illness in the United States, The produce safety project at Georgetown University, (2010).
21 Centers for Disease Control and Prevention (CDC): http://www.cdc.gov/ecoli/general
22 Walsh, K.A., Bennett, S.D., Mahovic, M. and Gould, L.H.: Outbreaks associated with cantaloupe, watermelon, and honeydew in the United States, 1973-2011. Foodborne Pathog Dis, 11, 945-952 (2014).   DOI
23 Food standards agency: Health protection agency advisory committee on the microbiological safety of food information paper 'Microbiological status of RTE fruit and vegetables' (2005).
24 Centers for Disease Control and Prevention (CDC): Foodborne outbreak tracking and reporting (available at http://wwwn.cdc.gov/foodborneoutbreaks/Default.aspx (2015).
25 Samadpour, M., Barbour, M.W., Nguyen, T., Cao, T.M., Buck, F., Depavia, G.A., Mazengia, E., Yang, P., Alfi, D., Lopes, M. and Stopforth, J.D.: Incidence of enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms. J Food Prot, 69, 441-443 (2006).   DOI
26 Morris, C.E. and Monier, J.M.: The ecological significance of biofilm formation by plant-associated bacteria. Annu Rev Phytopathol., 41, 429-453 (2003).   DOI
27 Chikthimmah, N., LaBorde, L.F. and Beelman, R.B.: The effect of washing and slicing operations on the survival behavior of Listeria monocytogenes and Salmonella sp in fresh mushrooms during postharvest storage. Mushroom News, 9, 4-13 (2007).
28 Whipps, J.M., Hand, P., Pink, D.A. and Bending, G.D.: Human pathogens and the phyllosphere. Adv Appl Microbiol., 64, 183-221 (2008).
29 Li, D., Friedrich, L.M., Danyluk, M.D., Harris, L.J. and Schaffner, D.W.: Development and validation of a mathematical model for growth of pathogens in cut melons. J Food Prot, 76, 953-958 (2013).   DOI
30 Berrada, H., Soriano, J.M., Pico, Y. and Manes, J.: Quantification of Listeria monocytogenes in salads by real time quantitative PCR. Int J Food Microbiol, 107, 202-206 (2006).   DOI
31 Painter, J.A., Hoekstra, R.M., Ayers, T., Tauxe, R.V., Braden, C.R., Angulo, F.J. and Griffin, P.M.: Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998-2008. Emerg Infect Dis, 19, 407-415 (2013).   DOI
32 Lee, K.I., Han, H.S. and Son, E.Y.: Analysis of Korean food consumption trends. Korea Rural Economic Institute, R560 (2007).
33 Rickman, J.C., Bruhn, C.M. and Barrett, D.M.: Nutritional comparison of fresh, frozen, and canned fruits and vegetables II. Vitamin A and carotenoids, vitamin E, minerals and fiber. J Sci Food Agric, 87, 1185-1196 (2007).   DOI
34 Berger, C.N., Sodha, S.V., Shaw, R.K., Griffin, P.M., Pink, D., Hand, P. and Frankel, G.: Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ Microbiol, 12, 2385-2397 (2010).   DOI
35 Beuchat, L.R. and Scouten, A.J.: Combined effects of water activity, temperature and chemical treatments on the survival of Salmonella and Escherichia coli O157:H7 on alfalfa seeds. J Appl Microbiol, 92, 382-395 (2002).   DOI
36 Bennett, S.D., Walsh, K.A. and Gould, L.H.: Foodborne disease outbreaks caused by Bacillus cereus, Clostridium perfringens, and Staphylococcus aureus--United States, 1998- 2008. Clin Infect Dis.: an official publication of the Infectious Diseases Society of America, 57, 425-433 (2013).   DOI