Browse > Article
http://dx.doi.org/10.13103/JFHS.2015.30.1.51

Profiles of Toxin genes and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Perilla Leaf Cultivation Area  

Kim, Se-Ri (Microbial Safety Team, Department of Crop Life Safety, NAAS, RDA)
Cha, Min-Hee (Microbial Safety Team, Department of Crop Life Safety, NAAS, RDA)
Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
Shim, Won-Bo (World Institute of Kimchi)
Publication Information
Journal of Food Hygiene and Safety / v.30, no.1, 2015 , pp. 51-58 More about this Journal
Abstract
Thirty one of Staphylococcus aureus isolated from perilla leaf cultivation areas in Miryang were investigated on the characteristics, such as enterotoxin genes and antibiotic susceptibility. Five toxin genes (sea, seb, sec, sed, and see) were examined by PCR method. Disc diffusion method was used to examine the antibiotic susceptibility of S. aureus by using 18 types of antibiotic discs with different concentrations. Among enterotoxin-encoding genes, sea and sed genes were co-detected from 4 isolates (12.9%), sed gene was founded in 9 isolates (29.0%), and see gene was founded in 1 isolate (3.2%). However seb and sec and tsst were not detected in any isolates. As a result of antibiotic susceptibility test, 7 isolates (22.6%) were resistant to 12 antibiotics (penicillin, ampicillin, oxacillin, amoxicillin-clavulanic acid, cefazolin, cephalothin, imipenem, gentamicin, tetracycline, ofloxacin, norfloxacin, and erythromycin). 2 isolates (6.5%) were resistant to 5 antibiotics (penicillin, ampicillin, amoxicillin-clavulanic acid, gentamycin, and telithromycin). MRSA (Methicilline Resistant Staphylococcus aureus) was founded in packing vinyl, hands, and perilla leaves.
Keywords
perilla leaves; S. aureus; toxigenic genes; antibiotics susceptibility;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Sevell, A.M., Farber, J.M.: Foodborne outbreaks in Canada linked to produce. J. Food Prot., 64, 1863-1877 (2001).
2 Choi J.W., Park S.Y., Yeon J.H., Lee M.J., Chung D.H., Lee K.H., Kim M.G., Lee D.H., Kim K.S., Ha S.D.: Microbial contamination levels of fresh vegetables distributed in markets. Korean J. Food Hyg. Safety., 20, 43-47 (2005).
3 FDA. Guidance for industry, Guide to minimize microbial food safety hazard for fresh fruits and vegetables. Available From: http://csan.fda.gov. Accessed Oct. 26, 2005.
4 Burnett, S.L., Beuchat, L.R.: Human pathogens associated with raw produce and unpasteurized juices and difficulties in decontamination. J. Ind. Microbiol. Biotechnol., 27, 104-110 (2001).   DOI
5 Reina, L.D., Fleming, H.P., Breidt, F.J.R.: Bacterial contamination of cucumber fruit through adhesion. J. Food Prot., 65, 1881-1887 (2002).
6 Suk S.U., Park S.C.: Staphylococcal infections. J. Infection., 17, 115-122 (1985).
7 Jang D.S., Sin D.H., Chung D.H., Kim C.M., Lee I.S.: Food hygienic. Jeongmungak Co., Seoul, Korea. pp. 71-111. 2002
8 Chen, T.R., Hsiao, M.H., Chiou, C.S., Tsen, H.Y.: Development and use of PCR primers for the investigation of $C_{1}$, $C_{2}$ and $C_{3}$ enterotoxin types of Staphylococcus aureus strains isolated from food-borne outbreaks. Int. J. Food Microbiol., 71, 63-70 (2001).   DOI
9 Lee E.J.: The effect of temperature and time on the multiplication of Staphylococcus in foods. Korean Journal of Public Health., 9, 381-387 (1972).
10 Dinges, M.M., Orwin, P.M., Schlievert, P.M:. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev., 13, 16-34 (2000).   DOI
11 Loir, Y.L., Baron, F., Gautier, M.: Staphylococcus aureus and food poisoning. Genet. Mol. Res., 2, 63-76 (2003).
12 Kim S.H., Ryu J.H., Kim M.S., Choi H.J.: The risk factors and prognosis of methicillin-resistant Staphylococcus aureus bacteremia : focus on nosocomial acquisition. The Korean Journal of Medicine., 71, 405-414 (2006).
13 Chang, S., Suvert, D.M., Hageman, J.C., Boulton, M.L., Tenover, F.C., and Pounch, D.F.: Infection with vancomycinresistant Staphylococcus aureus containing the vanA resistance gene. N. Engl. J. Med., 348, 1342-1347 (2003).   DOI
14 Boehme, S., Werner, G., Klare, I., Reissbrodt, R., Witte, W.: Occurrence of antibiotic-resistant enterobacteria in agricultural foodstuffs. Mol. Nutr. Food Res., 48, 522-531 (2004).   DOI
15 Kang C.S.: Study on the resistance and prevention of antibiotics. Patent 21., 54, 37-43 (2004).
16 Anonymous. Guidelines for effectiveness testing of surgical hand scrub (glove juice test). Fed. Regist. 43, 1242-1243 (1978).
17 KFDA. Korean Food code. Korea Food and Drug Administration, Seoul, Korea. pp. 75-105 (2002).
18 Karsten, B., Ricarda, R., Georg, P.: Rapid and specific detection of toxic Staphylococcus aureus : Use of two multiplex PCR enzyme immunoassays for amplification and hybridization of Staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J. Clin. Microbiol., 43, 2548-2553 (1998).
19 Yang, I.C., Shih, D.Y., Huang, T.P., Huang, Y.P., Wang, J.Y., Pan, T.: Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. J. Food Prot., 68, 2123-2130 (2005).
20 Tsen, H.Y., Chen, T.R.: Use of the polymerase chain reaction for specific detection of type A, D, and E enterotoxigenic Staphylococcus aureus in foods. Appl. Microbiol. Biotechnol, 37, 685-690 (1992).
21 Monday, S.R., Bohach, G.A.: Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in Staphylococcal isolates. J. Clin. Micobiol., 37, 3411-3414 (1999).
22 NCCLS. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard-9th ed. Clinical and Laboratory Standards Institute, Wayne, PA, USA. pp. 6-80 (2006).
23 Kim J.S., Bang O.K., Chang H.C.: Examination of microbiological contamination of ready-to-eat vegetable salad. J. Fd Hyg, Safety., 19, 60-65 (2004).
24 Jung S.H., Hur M.J., Ju J.H., Kim K.A., Oh S.S., Go J.M., Kim Y.H., Im J.S.: Microbiological evaluation of raw vegetables. J Food Hyg Safety., 21, 250-257 (2006).
25 Thunberg, R.L., Tran, T.T., Bennett, R.W., Matthews, R.N., Belay, N.: Microbial evaluation of selected fresh produces obtained at retail markets. J. Food Prot., 65, 677-682 (2002).
26 Jung H.J., Cho J.I., Park S.H., Ha S.D., Lee K.H., Kim C.H., Song E.S., Chung D.H., Kim M.G., Kim K.Y., Kim K.S.: Genotypic and phenotypic characteristics of Staphylococcus aureus isolates from lettuces and raw milk. Korean. J. Food Sci. Technol., 37, 134-141 (2005).
27 Kim J.S., Lee J.H., Kim J.H., Choi J.M., Kim S.R., Ha S.D., Kim K.S., Lee K.H., Kim M.G., Kim K.Y., Kim C.H., Chung D.H.: Characteristics of enterotoxigenic genes and antibiotic susceptibility of Staphylococcus aureus isolated from Tomato Farms in Western Gyeongnam. Korean J. Food Sci Technol., 38, 295-303 (2006).
28 Tan S.L., Lee H.Y., Mahyudin N.A.: Antimicrobial resistance of Escherichia coli and Staphylococcus aureus isolated from food handler's hands. Food Control, 44, 203-207 (2014).   DOI
29 Park S.H., Kim J.S., Kim K.Y., Chung D.H., Sim W.B.: Identification of toxin gene and antibiotic resistance of staphylococcus aureus isolated from agricultural product cultivation environments. J. Environ Health Sci., 39, 465-473 (2013).
30 Kim S.R., Shim W.B., Kim J.H., Hwang S.J., Park S.J., Ha S.D., Kim K.S., Lee K.H., Kim M.G., Kim K.Y., Kim C.H. Chung D.H.: Screeing of Staphylococcus aureus and Staphylococcal enterotoxin a, b, c gene in strain isolated from strawberry farms in Western Gyeongnam. Korean J. Food Sci. Technol., 37, 321-327 (2005).
31 Aydin, A., Sudagidan, M., Muratoglu, K.: Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Refion of Turkey. Int. J. food Microbiol., 148, 99-106 (2011).   DOI
32 Cho Y.S., Lee J.Y., Lee M.K., Sin D.B., Kim D.H., Park K.M.: Prevalence and characterization of Staphylococcus aureus pathogenic factors isolated from various foods in Korea. Korean J. Food Sci. Technol., 43, 648-665 (2011).   DOI
33 Crago B., Ferrato C., Drews S.J., Svenson L.W., Tyrrell G., Louie M.: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in food samples associated with foodborne illness in Alberta, Canada from 2007 to 2010. Food Microbiol., 32, 202-205 (2012).   DOI
34 Ha D.J., Kim Y.C., Kim Y.J.: Management of infection for methicillin resistant Staphylococcus aureus at an orthopaedic surgery department. J Korean Orthop Assoc., 38, 34-38 (2003).
35 Tsen, H.Y., Yu, G.K., Wang, K.C., Wang, S.J., Chang, M.Y., Lin, L.Y.: Comparison of the enterotoxigenic types, toxic shock syndrome toxin 1(TSST-1) strains and antibiotic susceptibilities for enterotoxigenic Staphylococcus aureus strains isolated from food and clinical samples. Food Microbiol., 15, 33-41 (1998).   DOI