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http://dx.doi.org/10.13103/JFHS.2015.30.1.103

Growth Inhibition of Listeria monocytogenes by Weissella spp. from Kimchi Through Real-time PCR  

Lee, Young-Duck (Department of Food Science and Engineering, Seowon University)
Kim, Dae-Yong (Department of Pharmaceutical Science and Engineering, Seowon University)
Park, Jong-Hyun (Department of Food Science and Biotechnology, Gachon University)
Publication Information
Journal of Food Hygiene and Safety / v.30, no.1, 2015 , pp. 103-108 More about this Journal
Abstract
Weissella spp. from traditional Korean foods of Kimchi were isolated and characterized against food-borne pathogenic Listeria monocytogens. The isolates were identified as W. cibaria 0D17 and W. confusa 0D23 from Kimchi by the biochemical characteristics and 16S DNA sequencing. The culture solutions of the isolates adjusted to pH 7.0 showed L. monocytogens inhibition. To analyze the quantitative detection of L. monocytogenes, real-time PCR was performed according to the SYBR Green I method. The isolates grew well and L. monocytogens did not grow during the co-culture with those strains at $37^{\circ}C$. Therefore, W. cibaria 0D17 and W. confusa 0D23 might be the candidates as the functional lactic acid bacteria for improving food safety.
Keywords
Weissella spp.; L. monocytogenes; Kimchi; Real-time PCR; Inhibition;
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