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http://dx.doi.org/10.13103/JFHS.2014.29.4.356

Carex pumila Extract Supresses Mast Cell Activation and IgE-Mediated Allergic Response in Mice  

Lim, Hannah (College of Pharmacy, Duksung Women's University)
Kim, Young Mi (College of Pharmacy, Duksung Women's University)
Publication Information
Journal of Food Hygiene and Safety / v.29, no.4, 2014 , pp. 356-362 More about this Journal
Abstract
Allergic diseases have increased rapidly over the past decades, affecting an estimated 20~30% of the population in developed countries. In this study, we investigated whether or not a typical costal sand dune plant Carex pumila (CPE) suppresses the activation of mast cells and IgE-mediated allergic response in vitro and in vivo. As the results, the extract of Carex pumila inhibited antigen-stimulated degranulation in RBL-2H3 cells and Bone marrow-derived mast cells (BMMCs), and IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. CPE also suppressed the production of pro-inflammatory cytokines, TNF-${\alpha}$ and IL-4, in antigen-stimulated mast cells. As its mechanism of action, CPE inhibited the activation of Syk in $Fc{\varepsilon}RI$-mediated signalling pathway, and that of LAT, a downstream adaptor molecule of Syk, in a dose-dependent manner. CPE also suppressed the activation of mitogen-activated protein (MAP) kinases, p38, ERK1/2, JNK, and Akt. Altogether, CPE inhibited mast cell activation and IgE-mediated allergic response by antigen through suppressing the activation of Syk. These results suggest that CPE may be useful for the treatment of allergic diseases.
Keywords
Carex pumila; Allergy; Passive Cutaneous Anaphylaxis; IgE; Mast Cells; Syk Kinase;
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1 Ebo D.G. and Stevens W.J. IgE-mediated food allergyextensive review of the literature. Acta Clin Belg 56, 234-247 (2001).   DOI
2 Alma J.N., Ferdi E., Leon M.K., Johan G., Frans P.N. and Frank A.R. Mechanisms of allergy and asthma. Eur J Pharmacol 585, 354-360 (2008).   DOI   ScienceOn
3 Toshio T. Efficacy of Flavonoids for Patients with Japanese Cedar Pollinosis. Current Insights in Pollen. Allergens 103-122 (2012).
4 Galli, S. J. New concepts about the mast cells. New Eng. J. Med. 328, 257-270 (1993).   DOI   ScienceOn
5 Paolini R., Jouvin M.H. and Kinet J.P. Phosphorylation and dephosphorylation of the high-affinity receptor for immunoglobulin E immediately after receptor engantigenement and disengantigenement. Nature 353, 855-858 (1991).   DOI
6 Wong B.R., Grossbard E.B., Payan D.G., and Masuda E.S. Targeting Syk as a treatment for allergic and autoimmune disorders. Expert Opin Invest Drugs 13, 743-762 (2004).   DOI   ScienceOn
7 Rivera J and Gilfillan A.M. Molecular regulation of mast cell. activation. J Allergy Clin Immunol 117, 1214-1225 (2006).   DOI   ScienceOn
8 Gilfillan A.M. and Tkaczyk C. Integrated signalling pathways for mast-cell activation. Nat Rev Immunol 6, 218-230 (2006).   DOI   ScienceOn
9 김경만, 알레르기 반응의 신호전달체계 및 조절기능에 관한 연구, 보건복지부 보건의료개발사업 보고서, 2004.
10 환경부, 자연방파제 해안사구, 2011.
11 민병미, 한국의 해안 사구식물, 2006.
12 강영희, 생명과학대사전, 2008.
13 Beaven, M. A. Our perception of the mast cell from Paul Ehrlich to now. Eur. J. Immunol. 39, 11-25 (2009).   DOI
14 Voehringer, D. Protective and pathological roles of mast cells and basophils. Nat. Rev. Immunol. 13, 362-375 (2013).   DOI   ScienceOn
15 Wedemeyer J., Tsai M. and Galli S. J. Roles of mast cells and basophils in innate and acquired immunity. Curr. Opin, Immunol. 12, 624-631 (2000).   DOI   ScienceOn
16 Sada, K., Takano, T., Yanatigeni, S. and Yamamura, H Structure and function of Syk protein-tyrosine kinase. J. Biochem. 130, 177-186, (2001).   DOI
17 Theohardies, T.C. and Kalogeromitros, D. The dritical role of mast cells in allergy and inflammation. Ann. N.Y. Acad. Sci. 1088, 78-99 (2006).   DOI   ScienceOn
18 Chu, D. H., Morita, C.T. and Weiss, A. The syk family of protein tyrosin kinase in T-cell activation and development. Immunol. Rev, 165, 167-180 (1998).   DOI