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http://dx.doi.org/10.13103/JFHS.2014.29.2.131

Simultaneous Determination of Penicillin Antibiotics in Meat using Liquid Chromatography Tandem Mass Spectrometry  

Kim, Myeong-Ae (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Yoon, Su-Jin (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kim, MeeKyung (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Cho, Yoon-Jae (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Choi, Sun-Ju (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Chang, Moon-Ik (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Lee, Sang-Mok (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kim, Hee-Jeong (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Jeong, Jiyoon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Rhee, Gyu-Seek (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Lee, Sang-Jae (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation)
Publication Information
Journal of Food Hygiene and Safety / v.29, no.2, 2014 , pp. 131-140 More about this Journal
Abstract
The objective of this study was to develop a simultaneous method of 8 penicillin antibiotics including amoxicillin, ampicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin, penicillin G and penicillin V in meat using LC-MS/MS. The procedure involves solid phase extraction with HLB cartridge and subsequent analysis by LC-MS/MS. To optimize MS analytical condition of 8 compounds, each parameter was established by multiple reaction monitoring in positive ion mode. The chromatographic separation was achieved on a $C_{18}$ column with a mobile phase of 0.05% formic acid and 0.05% formic acid in acetonitrile at a flow rate of 0.2 mL/min for 20 min with a gradient elution. The developed method was validated for specificity, linearity, accuracy and precision in beef, pork and chicken. The recoveries were 71.0~106%, and relative standard deviations (RSD) were 4.0~11.2%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.003~0.008 mg/kg and 0.01~0.03 mg/kg, respectively, that are below maximum residue limit (MRL) of the penicillins. This study also performed survey of residual penicillin antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Penicillins were not found in all the samples except a sample of pork which contained cloxacillin (concentration of 0.08 mg/kg) below the MRL (0.3 mg/kg).
Keywords
penicillin antibiotics; meat; validation; LC-MS/MS;
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Times Cited By KSCI : 5  (Citation Analysis)
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