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http://dx.doi.org/10.13103/JFHS.2014.29.2.123

Simultaneous Determination of Aminoglycoside Antibiotics in Meat using Liquid Chromatography Tandem Mass Spectrometry  

Cho, Yoon-Jae (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Choi, Sun-Ju (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kim, Myeong-Ae (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kim, MeeKyung (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Yoon, Su-Jin (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Chang, Moon-Ik (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Lee, Sang-Mok (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kim, Hee-Jeong (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Jeong, Jiyoon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Rhee, Gyu-Seek (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Lee, Sang-Jae (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation)
Publication Information
Journal of Food Hygiene and Safety / v.29, no.2, 2014 , pp. 123-130 More about this Journal
Abstract
A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.
Keywords
aminoglycoside antibiotics; meat; validation; LC-MS/MS;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Park M. S., Lee J. J., Myung S. W.: Analysis of phoxim residue in animal food production (cattle and pig) by LC/ESIMS/ MS. J. Korean Chem. Soc. 55, 626-632 (2011).   DOI
2 권기성 등. 식품 중 잔류동물용의약품 안전성 연구 (I); 2011년 잔류동물용의약품 시험법 개발 및 잔류실태조사. 식품의약품안전청 연구보고서 (2011).
3 Codex guidelines for the establishment of a regulatory programme for control of veterinary drug residues in foods. CAC/GL 16. 1-46 (1993).
4 Guidelines on Good Lavoratory Practice in Pesticide Residue Analysis. CAC/GL 40-1993 (2010).
5 Korea Meat Trade Association (KMTA). Information and data of agricultural statistics of Korea. Avaliable from http://kmta.or.kr/html/sub6-1.html?scode=6. Oct. 20 (2012).
6 Kim H. Y., Chung S. Y., Choi S. H., Lee J. S., Choi I. S., Cho M. J., Shin M. S., Song J. S., Choi J. C., Park H. O., Ha S. C., Shin I. S., Seo E. C.: Monitoring of veterinary drug residues in foods produced in Korea. Korean J. Food Sci. Technol. 42, 653-663 (2010).
7 Hah D. S., Kim J. S., Kim G. S.: Simultaneous quantification of sulfonamide and tetracyclines in fish muscle tissue by matrix solid phase dispersion (MSPD) extraction and HPLC. J .Food Hyg. Safe. 12, 117-124 (1997).
8 신호철, 박관하: 동물용의약품 잔류실태 조사. 식품의약품안전청 연구보고서 (2006).
9 Guillemot D.: Antibiotic use in humans and bacterial resistance. Curr. Opin. Microbiol. 2, 494-498 (1999).   DOI   ScienceOn
10 이태식: 수산용 항생제 관리시스템 구축. 식품의약품안전청 연구보고서 (2006).
11 Yanfei T., Dongmei C., Huan Y., Lingli H., Ahaoying L., Xiaoqin C., Caixia Y., Yuanhu P., Zhenli L., Zonghu Y.: Simultaneous determination of 15 aminoglycoside(s) residues in animal derived food by automated solid-phase extraction and liquid chromatography-tandem mass spectrometry. Food Chem.. 135, 676-683 (2012).   DOI   ScienceOn
12 식품공전: 식품의약품안전처 (2013).
13 Kim M. K., Cho B. H., Lim C. M., Kim D. G., Yune S. Y., Shin J. Y., Bong Y. H., Kang J. W., Kim M. A., Son S. W. : Chemical Residues and Contaminants in Foods of Animal Origin in Korea during the Past Decade. J. of Agri. & Food Chem. 61, 293-2298 (2013).
14 이광근, 정윤희: 식품 중 항생제 잔류량 모니터링. 식품의약품안전청 연구보고서 (2005).
15 Roh Y. S., Baek G. J., Kim S. Y., Choi E. Y., Seo H. S., Hur B. H., Joung D. S.: Simultaneous determination of aminoglycoside antibiotics by HPLC/MS. Korean J. Vet. Serv. 30, 1-12 (2007).
16 Kaufmann A., Butcher P., Maden K.: Determination of aminoglycoside residues by liquid chromatography and tandem mass spectrometry in a variety of matrices. Anal. Chim. Acta. 711, 6-53 (2012).
17 Sara B., Roverta C., Antonio D. C., Aldo L., Monica M., Manuela N.: Simple confirmatory assay for analyzing residues of aminoglycoside antibiotics in bovine milk: hot water extraction followed by liquid chromatography-tandem mass spectrometry. J. Chromatogr. A. 1067, 93-100 (2005).   DOI
18 Zhu W. X., Yang J. Z., Wei W., Liu Y. F., Zhang S. S.: Simultaneous determination of 13 aminoglycoside residues in foods of animal origin by liquid chromatography-elecrosparay ionization tandem mass spectrometry with two consecutive solidphase extraction steps. J. Chromatogr. A. 1207, 29-37 (2008).   DOI
19 Umezawa, H., Hooper I. R.: Aminoglycoside antibiotics, Springer-Verlag, p. 293 (1982).
20 Turnipseed S. B., Clark S. B., Karbiwnyk C. M., Anderse W. C., Miller K. E., Madson M. R.: Analysis of aminoglycoside resideues in bovine milk by liquid chromatography electrospray ion trap mass spectrometry after derivatization with phenyl isocyanate. J. Chromatogr. B. 877, 1487-1493 (2009).   DOI
21 Byun J. Y.: Update of Research on Aminoglycoside toxicity. Korean J Otorhinolaryngol-Head Neck Surg. 55, 1-7 (2012).   DOI
22 Cox E. C., White, J. R., Flaks, J. G.: Streptomycin action and the ribosome. Proc. Natl Acad. Sci. 51, 703-709 (1964).   DOI