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http://dx.doi.org/10.13103/JFHS.2013.28.2.124

Development of PCR Method for Rapid Detection of Allergic Materials in Foods  

Park, Yong-Chjun (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Kim, Mi-Ra (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Shin, Jun-Ho (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Kim, Kyu-Heon (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Lee, Jae-Hwang (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Cho, Tae-Yong (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Lee, Hwa-Jung (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Lee, Sang-Jae (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Han, Sang-Bae (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
Publication Information
Journal of Food Hygiene and Safety / v.28, no.2, 2013 , pp. 124-129 More about this Journal
Abstract
The method for detection foods containing allergenic materials by PCR was developed in this study. To detect allergenic raw material from processed food, species specific primer which up to 200bp for PCR product were designed or selected from advanced research. As target materials, 14 items were selected (12 target materials for allergen in Korea, 2 target materials for allergen in foreign countries). The amplicon size for eggs, milk, buckwheat, peanuts, beans, wheat, mackerel, crab, shrimp, pork, peach, tomato, almond, and sesame were confirmed 281, 131, 138, 120, 118, 127, 211, 174, 231, 138, 174, 132, 103, and 220bp, respectively. And any non-specific bands were not detected among each others. Detection method for allergenic material developed in this study could be used to investigate inaccurate goods for allergen labeling or non-intentional contaminant during processed foods manufacturing. In addition, the system will be usefully to detection accurate allergenic raw materials of export for other countries.
Keywords
Allergen; PCR (Polymerase Chain Reaction); Species-specific primer;
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1 Wunsch, A. and Hormaza, J.I.: Molecular characterization of sweet cherry (Prunnus avium L.) genotypes using peach [Prunnus persica (L.) Batsch] SSR sequences, Heredity, 89, 56-63 (2002).   DOI   ScienceOn
2 Shirasawa, K., Asamizu, E., Fukuoka, H., Ohyama, A., Sato, S., Nakamura, Y., Tabata, S., Sasamoto, S., Wada, T., Kishida, Y., Tsuruoka, H., Fujishiro, T., Yamada, M. and Isobe, S.: An interspecific linkage map of SSR and intronic polymorphism markers in tomato, Theor Appl Genet., 121, 731-739 (2010).   DOI   ScienceOn
3 식품의약품안전평가원, 2012, 가짜식품 판별 검사 지침서(I) (2011).
4 식품공전, 제 10 일반시험법, 8 식품표시관련시험법 (2011).
5 He, G., Ronghua, M., Newman, M., Gao, G., Pittman, R.N. and Prakash, C.S.: Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.), BMC Plant Biology, 3 (2003).
6 Wei, W., Qi, X., Wang, L., Zhang, Y., Hua, W., Li, D., Haixia, L. and Zhang X.: Characterization of the sesame(Sesamum indicum L.) global transcriptome using Illumina paired-end sequencing and development of ETS-SSR markers, BMC Genomics, 12, 451 (2011).   DOI
7 Frew, A.J.: Food allergy and intolerances, Allergy, 52, 675 (1997).   DOI
8 한국소비자원 소비자위해감시시스템(http://www.kca.go.kr)
9 식품의약품안전청, 2005. 식품의약품안전청고시 제2005-12호.
10 식품의약품안전청, 2008. 일본의 알레르기 유발물질 검사법 규정: 번역집, 63.
11 Roland, E.P. and Elke, A.K.: Polymerase Chain Reaction Techniques for Food Allergy Detection, J. AOAC Int., 87, 139 (2004).
12 Hebert, P.D.N., Alina, C., Shelley, L.B. and Jeremy, R.: Biological identifications through DNA Barcodes, Proc. R. Soc. Lond. B., 270, 313-321 (2003).   DOI   ScienceOn
13 Michael, T.M., Michael, B., Gregory, T.R. and Alfried, P.V.: DNA-based species delineation in tropical beetles using mitochondrial and nuclear markers, Philos. Trans. R. Soc., 360, 1925-1933 (2005).   DOI   ScienceOn
14 Tomoaki, M., Atsushi, A., Takuma, T., Sumitaka, Y., Yutaka, O. and Yoshida, M.: Identification of animal species using the partial sequences in the mitochondrial 16S rRNA Gene, Legal Med., 11, 449-450 (2009).   DOI   ScienceOn
15 http://www.inspection.gc.ca
16 http://www.mhlw.go.jp
17 King, N., Helm, R. and Stanley, S.: Allergenic characteristics of a modified peanut allergen, Mol. Nutr. Food Res., 49, 963-971 (2005).   DOI   ScienceOn
18 Sampson, H.A.: Update on food allergy, J. Allergy Clin. Immunol., 113, 805-819 (2004).   DOI   ScienceOn
19 http://www.fda.gov
20 http://www.emea.europa.eu/ema
21 Samuel, B., Lehrer, W., Elliott, H. and Gerald, R.: Why are some proteins allergenic? Implications for biotechnology, Crit. Rev. Food Sci. Nut., 36, 553-564 (1996).   DOI   ScienceOn
22 Lee, J.H., Song, K.Y., Hyeon, J.Y., Hwang, I.G., Kwak, H.S., Han, J.A., Chung, Y.H. and Seo, K.H.: Comparison of Standard Culture Method and Real-time PCR Assay for Detection of Staphylococcus aureus in Processed and Unprocessed Foods, Korean J. Food Sci. Ani. Resour., 30, 410-418 (2010).   과학기술학회마을   DOI   ScienceOn
23 Lee, S.Y., Jang, K.I., Woo, G.J., Kwak, H.S. and Kim, K.Y.: Development of Protocol for the Effective Detection of Feline Calicivirus as Norovirus Surrogate in Oyster and Lettuce, Korean J. Food Sci. Techinol., 39, 71-76 (2007).   과학기술학회마을
24 Park, Y.C., Ahn, C.Y., Jin, S.O., Lim, J.Y., Kim, K.H., Lee, J.H., Cho, T.Y., Lee, H.J., Park, K.S. and Yoon, H.S.: Identification of Raw Materials in Processed Meat Products by PCR Using Species-Specific Primer, J. Fd Hyg. Safety, 27, 68-73 (2012).   DOI   ScienceOn
25 Rakoczy-Trojanowska, M. and Bolibok, H.: Characteristics and comparison of three classes of microsatellite-based maskers and their application in plants, Cell Mol Biol Lett., 9, 221-238 (2004).
26 Park, Y.C., Lim, J.Y., Kim, M.R., Park, Y.E., Lim, J.D., Hwang, C.R., Kim, K.H., Lee, J.H., Cho, T.Y., Lee, H.J., Lee, S.J. and Han, S.B.: Identification of Fauly Red Pepper Powder Containing Seasoned Red-pepper Sauce, J. Fd Hyg. Safety, 27, 182-187 (2012).   DOI   ScienceOn