Browse > Article
http://dx.doi.org/10.13103/JFHS.2012.27.1.063

Prevalence and Molecular Characterization of Tetracycline Resistance in $Enterococcus$ Isolates from Raw Milk Samples in Korea  

Kim, Ji-Hoon (Division of Animal Science, Sahmyook University)
Choi, Sung-Sook (College of Pharmacy, Sahmyook University)
Publication Information
Journal of Food Hygiene and Safety / v.27, no.1, 2012 , pp. 63-67 More about this Journal
Abstract
Antibiotic resistance in animal isolates of enterococci is a public health concern, because of the risk of transmission of antibiotic-resistant strains or resistance genes to humans through the food chain. This study investigated phenotypic and genotypic resistances profile of tetracycline in 245 $Enterococcus$ isolates from bovine milk. A total of 245 enterococci were isolated from 950 milk samples. The predominant strain was $E.$ $faecalis$ (n = 199, 81.2%) and $E.$ $faecium$ (n = 25, 10.2%). $E.$ $avium$ (n = 7, 2.9%), $E.$ $durans$ (n = 6, 2.5%), $E.$ $gallinarum$ (n = 4, 1.6%), and $E.$ $raffinosus$ (n = 4, 1.6%) were also isolated. Of the 245 enterococcal isolates 76.3% (n = 187) displayed tetracycline resistance (${\geq}16{\mu}g/ml$). Of the 187 tetracycline-resistant isolates, 83.4% (n = 156), 16.1% (n = 30), and 26.7% (n = 50) possessed the genes $tet$(M), $tet$(L), $tet$(S) respectively. While 3.2% (n = 6) of the tetracycline-resistant isolates possessed all three genes $tet$(M) + $tet$(L) + $tet$(S), 8.6% (n = 16), 16.0% (n = 30), and 2.7% (n = 5) of them possessed two genes $tet$(M) + $tet$(L), $tet$(M) + $tet$(S), and $tet$(L) + $tet$(S) respectively. The tetracycline resistance pattern investigated in this study was attributable mainly to the presence of $tet$(M).
Keywords
antibiotic resistance; enterococci; bovine milk; tetracycline;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Devriese, L.A., Collins, M.D. and Wirt, R.: The genus Enterococcus. In: Ballows, A., Truper, H. G., Dworkin, M., Harder, W., Schleifer, K. H., (Eds.) The Prokaryotes, 2nded., vol. 2, pp. 1465-1478 (1992), Springer-Verlag. New York.
2 Flahaut, S., Boutibonnes, P. and Auffray, Y.: Les enterocoques dans I'environnement proche de I'homme. Canad. J. Microbio. 43, 699-708 (1997).   DOI
3 Malani, P.N., Kauffman, C.A. and Zervos, M.J: Enterococcal disease, epidemiology and treatment. In: Gilmore, M. S. (Ed.) The Enterococci: pathogenesis, molecular biology and antimicrobiotic resistance. PP. 385-408 (2002). Amercan Society for Mcirobiology. Washington, DC
4 Mannu, L., Paba, A., Daga, E., Comunian, R., Zanetti, S., Dupre, I. and Sechi L.A.: Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int. J. Food Microbiol. 88, 291-304 (2003).   DOI
5 NORM. NORM-VET: Usage of antimicrobial agents and occurrence of antimicrobial resistance in Norway, pp. 1-72 (2003).
6 OIE. European Scientific Conference: The use of antibiotics in animal ensuring the protection of public health. pp. 8-142 (2001).
7 Sorensen, T.L., Blom, M., Monnet, D.L., Frimodt-Moller, N., Poulsen, R.L. and Espersen, F.: Transient intestinal carriage after ingestion of antibiotic-resistant Enterococcus faecium from chicken and pork. N. Engl. J. Med. 345, 1161-1166 (2001).   DOI
8 Witte, W., Klare, I. and Werner, G.: Selective pressure by antibiotics as feed additives. Infection. 29, suppl2, S35-38 (1999).
9 Lim, S.K., Lee, H.S., Byun, J.R., Park, S.Y. and Jung, S.C.: Antimicrobial resistance of commensal bacteria isolated from food-producing animals 1. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from cattle fecal samples. Kor. J. Vet. Pub. Health. 31(1), 21-29 (2007).
10 Kim, A.R., Cho, Y.M., Lim, S.K., Her, M., Jeong, W.S., Jung, S.C. and Kwon, J.H.: Antimicrobial resistance of commensal bacteria isolated from food-producing animals 3. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from chicken fecal samples. Kor. J. Vet. Pub. Health. 31(1), 41-49 (2007).
11 Roberts, M.C.: Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbiol. Rev. 19(1), 1-24 (1996).
12 Chopra, I. and Roberts, M.C.: Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev. 65(2), 232-260 (2001).   DOI
13 Huys, G., D'Haene, K., Collard, J.M. and Swings, J.: Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl. Environ. Microbiol. 70, 1555-1562 (2004).   DOI
14 Aarestrup, F.M., Hasman, H., Jensen, L.B., Moreno, M., Herrero, I.A., Domínguez, L., Finn, M. and Franklin, A.: Antimicrobial resistance among enterococci from pigs in three European countries. Appl. Environ. Microbiol. 68, 4127-4129 (2002).   DOI
15 Barbosa, J., Ferreira, P. and Teixeira, P.: Antibiotic susceptibility of enterococci isolated from traditional fermented meat products. Food. Micorbiol. 26, 527-532 (2009).   DOI
16 Hershberger, E., Oprea, S.F., Donabedian, S.M., Perri, M., Bozigar, P., Bartlett, P. and Zervos, M.J.: Epidemiology of antimicrobial resistance in enterococci of animal origin. J. Antimicrob. Chemother. 55, 127-130 (2005).   DOI
17 Nam, H.M., Lim, S.K., Moon, J.S., Kang, H.M., Kim, J.M., Jang, K. C., Kim, J.M., Kang, M.I., Joo, Y.S. and Jung, S.C.: Antimicrobial resistance of enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health. 57(7-8):e59-64 (2010)   DOI
18 CLSI.: Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement. Clinical and Laboratory Standards Institute. ASM press, Washington DC, USA. (2007)
19 Kim, C.M., Kang, S.J., Lee, B.J., Lee, S.J. and Yuk, D.S.: Prevalence and molecular characterization of tetracyclineresistant Enterococcus isolates from live stock. Kor. J. Vet. Serv. 33(2) 143-149 (2010).
20 Jeong, K.O., Heo, J.H., Lee, J.M., Yun, I.R., Choi Y.J. and Kim, J.S.: Surveillance of antimicrobial resistance ratio of E. coli and Enterococcus spp. isolated from fecal and carcasses of pigs in slaughterhouse. Kor. J. Vet. Serv. 33(3), 241-248 (2010).
21 Korea Animal Health Products Association, http://www.kahpa.or.kr/