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Analysis of coenzyme Q 1O in dietary supplement by HPLC  

No, Ki-Mi (Korea Food & Drug Administration)
Leem, Dong-Gil (Korea Food & Drug Administration)
Kim, Mi-Gyeong (Korea Food & Drug Administration)
Park, Kyoung-Sik (Korea Food & Drug Administration)
Yoon, Tae-Hyung (Korea Food & Drug Administration)
Hong, Jin (Korea Food & Drug Administration)
Park, Sun-Young (Korea Food & Drug Administration)
Jeong, Ja-Young (Korea Food & Drug Administration)
Publication Information
Journal of Food Hygiene and Safety / v.26, no.1, 2011 , pp. 12-15 More about this Journal
Abstract
A method based on high-performance liquid chromatography (HPLC) with ultraviolet detection has been developed to quantify coenzyme Q10 (CoQ10) in raw materials and dietary supplements. Single-laboratory validation has been performed on the method to determine linearity, selectivity, accuracy, limits of quantification (LOQ) and repeatability for CoQ10. An excellent linearity (r=0.999) was observed for CoQ10 in the concentration range $15.625{\sim}500\;{\mu}g/ml$ in dietary supplement. Observed recovery of CoQ10 was found to be between 98.33 and 99.38%. LOQ was found to be $250\;{\mu}g/ml$ Repeatability precision for CoQ10 was between 0.15 and 0.21% relative standard deviation (RSD). Further, limited studies showed that some adulterants and degraded material could be satisfactorily separated from CoQ10 and identified.
Keywords
Coenzyme Q10; dietary supplements; HPLC; validation;
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