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Determination of Six Sweeteners in Children's Favorite Foods by HPLC-MS/MS  

Kim, Il-Young (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Du, Ok-Ju (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Sung-Dck (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Park, Young-He (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Mi-Sun (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Bea, Chung-Ho (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Chae, Young-Zoo (Food Analysis Team, Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Journal of Food Hygiene and Safety / v.25, no.2, 2010 , pp. 118-121 More about this Journal
Abstract
A HPLC-MS/MS method was developed for simultaneous determination of six sweeteners (acesulfame-K, cyclamate, saccharin, sucralose, stevioside, aspartame) in children's favorite foods. The procedure involves an extraction of the six sweeteners with 50% methanol solution, sample clean-up using the Carrez clearing reagent and filtering with cartridge filter. The HPLC separation was performed on a Hypersil Gold (150 mm ${\times}$ 2.1 mm 5 um) column using the water/acetonitrile mobile phase (95:5). Mass spectrometric analysis was carried out using the TSQ Quantum Ultra operated in negative and positive ESI/SRM. With this method, good linear relationship, sensitivity and reproducibility were obtained. The spike recoveries of six sweeteners for 2 kinds of foods spiked into 0.4 mg/ kg ranged from 87.4 to 114.7%. The detection limits were above 0.02 mg/kg. The method has been applied to determination of six sweeteners in children's favorite foods.
Keywords
sweeteners; ESI; children's favorite foods;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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