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Micronucleus Test of Wild Ginseng Culture Extract Using the Marrow Cells in ICR Mice  

Song Si-Whan (Chemon Inc.)
Yang Deok Chun (Oriental Medicine Material and Processing Center, Kyung Hee University)
Choung Se Young (Department of Hygenic Chemistry, College of Pharmacy, Kyung Hee University)
Publication Information
Journal of Food Hygiene and Safety / v.20, no.1, 2005 , pp. 58-63 More about this Journal
Abstract
To assess clastogenic effects of the wild ginseng culture extract (WGCE) in vivo micronucleus test was performed using 7 weeks old ICR mice. At 24 hours after 2nd treatment with wild ginseng culture extract at the doses of 0, 500, 1,000, and 2,000 mg/kg/day by peritoneal route mice were sacrified and marrow cells were prepared for smear slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocyte(PCE), all treatment groups did not show statistically significant increase than negative control group. And there was no clinical sign connected with injection of wild ginseng culture extract. It was concluded that wild ginseng culture extract did not induce micronucleus in the marrow cells of ICR mice.
Keywords
wild ginseng culture extract; micronucleus; polychromate erythrocyte; marrow cell;
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1 Yamaguchi, H,. Matsuura, H., Kasai, R., Tanaka, O., Satake, M., Kodha, H., Izumi, H., Nuno, M., Katsuki, S. and Isoda, S.: Analysis of saponins of wild ginseng. Chem. Pharm. Bull., 36(10), 4177-4181 (1988)   DOI   ScienceOn
2 신미희: 산삼의 배양 및 그 응용에 관한 연구. 대한화장품학회지, 27(2), 45-56 (2001)
3 남기열: 최신고려인삼 (성분과 효능편). 천일인쇄사, pp. 247-252 (1996)
4 Zar JH: Bioststistical Analysis, 2nd Edition, Prentice-Hall International Editions (1990)
5 Lovell, D.P., Anderson, D., Albanese, R., Amphlett, G.E., Clare, G., Ferguson, R., Richold, M., Papworth, D.G. and Savage, J.R.K.: Statistical analysis on in vivo cytogenetic assays, In Statistical evaluation of mutagenicity test data (Kirkland, D.J. ed), Chambridge University Press, Cambridge, U.K., pp.184-232 (1998)
6 Lee, E.J., Jhao, H.L., Li, D.W., Jung, C.S., Kim, J.H. and Kim, Y.S.: Effect of MeOH extract of adventious root culture of Panax ginseng on hyperlipidemic rat induced by high fat-rich diet. Korean J. Pharmacogn,. 34(2), 179-184 (2003)
7 SAS Intitute: SAS/STAT User's guide, Version 6, 4th ed., Vol.1, Cary, NC., USA (1989a)
8 SAS Intitute: SAS/STAT User's guide, Version 6, 4th ed., Vol.2, Cary, NC., USA (1989b)
9 Alder, I.D.: Cytogenetic tests in mammals. In mutagenecity testing a practical approach. Venitt, S. Parry, JM (eds.), IRL press, Oxford, pp. 275-306 (1984)
10 Mizuno, M., Yamada, J., Terai, H., Kozukue, N., Lee, Y.S. and Tsuchida, H.: Differences in immunomodualating effects between wild and cultured Panax ginseng. Biochem. Biophys. Res. Commun., 200(3), 1672-1678 (1994)   DOI   ScienceOn
11 Heddle, J.A., Stuart, E., Salamone, M.F.: The bone marrow micronucleus test. In Handbook of mutagenicity test procedures, 2nd edition Elsevier Science Publishiers BV. pp.441-457 (1984)
12 Schmid, W.: The micronucleus test. Mutat. Res., 31:9-15 (1975)   DOI