Browse > Article
http://dx.doi.org/10.9721/KJFST.2022.54.4.398

Effects of black chokeberry on cholesterol metabolism in HepG2 cells  

Lee, Sang Gil (Department of Food Science and Nutrition, Pukyong National University)
Kim, Bohkyung (Department of Food Science and Nutrition, Pusan National University)
Publication Information
Korean Journal of Food Science and Technology / v.54, no.4, 2022 , pp. 398-402 More about this Journal
Abstract
Black chokeberry (Aronia melanocarpa), a rich source of polyphenols, exerts hypocholesterolemic effects. However, little is known about its effects on the regulation of the hepatic cholesterol metabolism and the underlying mechanisms. In the present study, the effects of polyphenol-rich black chokeberry extract (CBE) on hepatic cholesterol metabolism were investigated by measuring the expression of genes involved in the absorption, de novo synthesis, and efflux of cholesterol in HepG2 cells. There was a significant reduction in the expression levels of genes involved in cholesterol metabolism, the low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and sterol regulatory element-binding protein 2, in CBE-treated HepG2 cells. Meanwhile, CBE increased the expression levels of genes involved in cholesterol and bile acid efflux. The expression levels of mitochondrial fatty acid oxidation genes increased, whereas those of lipogenic genes decreased following CBE treatment. These data suggest that the consumption of black chokeberry may be beneficial for the prevention of hypercholesterolemia.
Keywords
black chokeberry; Aronia melanocarpa; choelsterol metabolism; hypocholesterolemia; HepG2 cells;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Madison BB. Srebp2: A master regulator of sterol and fatty acid synthesis. J. Lipid Res. 57: 333-335 (2016)   DOI
2 Millar JS, Cuchel M. Cholesterol metabolism in humans: a review of methods and comparison of results. Curr. Opin. Lipidol. 29: 1-9 (2018)   DOI
3 Ontawong A, Pasachan T, Trisuwan K, Soodvilai S, Duangjai A, Pongchaidecha A, Amornlerdpison D, Srimaroeng C. Coffea arabica pulp aqueous extract attenuates oxidative stress and hepatic lipid accumulation in HepG2 cells. J. Herb. Med. 29 (2021)
4 Quinones M, Miguel M, Aleixandre A. Beneficial effects of polyphenols on cardiovascular disease. Pharmacol. Res. 68: 125-131 (2013)   DOI
5 Ren K, Jiang T, Zhao GJ. Quercetin induces the selective uptake of HDL-cholesterol via promoting SR-BI expression and the activation of the PPARgamma/LXRalpha pathway. Food Funct. 9: 624-635 (2018)   DOI
6 Simonson W. Update on statin drugs for lipid disorders. Geriatr. Nurs. 39: 350-351 (2018)   DOI
7 Nelson RH. Hyperlipidemia as a risk factor for cardiovascular disease. Primary Care. 40: 195-211 (2013)   DOI
8 Shen WJ, Azhar S, Kraemer FB. SR-B1: a unique multifunctional receptor for cholesterol influx and efflux. Annu. Rev. Physiol. 80: 95-116 (2018)   DOI
9 Tangney CC, Rasmussen HE. Polyphenols, inflammation, and cardiovascular disease. Curr. Atheroscler Rep. 15: 324 (2013)   DOI
10 Temel RE, Brown JM. A new model of reverse cholesterol transport: enTICEing strategies to stimulate intestinal cholesterol excretion. Trends Pharmacol. Sci. 36: 440-451 (2015)   DOI
11 Wang DQ, Portincasa P, Tso P. Transintestinal cholesterol excretion: a secondary, nonbiliary pathway contributing to reverse cholesterol transport. Hepatology. 66: 1337-1340 (2017)   DOI
12 Jurikova T, Mlcek J, Skrovankova S, Sumczynski D, Sochor J, Hlavacova I, Snopek L, Orsavova J. Fruits of black chokeberry Aronia melanocarpa in the prevention of chronic diseases. Molecules. 22 (2017)
13 Virani SS, Alonso A, Aparicio HJ, Benjamin EJ, Bittencourt MS, Callaway CW, Carson AP, Chamberlain AM, Cheng S, Delling FN, Elkind MSV, Evenson KR, Ferguson JF, Gupta DK, Khan SS, Kissela BM, Knutson KL, Lee CD, Lewis TT, Liu J, Loop MS, Lutsey PL, Ma J, Mackey J, Martin SS, Matchar DB, Mussolino ME, Navaneethan SD, Perak AM, Roth GA, Samad Z, Satou GM, Schroeder EB, Shah SH, Shay CM, Stokes A, Van-Wagner LB, Wang NY, Tsao CW, American Heart Association Council on Epidemiology and Prevention Statistics Committee and Stroke Statistics Subcommittee. Heart disease and stroke statistics-2021 update: a report from the American Heart Association. Circulation. 143: e254-e743 (2021)
14 Zafra-Stone S, Yasmin T, Bagchi M, Chatterjee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol. Nutr. Food Res. 51: 675-683 (2007)   DOI
15 Tu L, Sun H, Tang M, Zhao J, Zhang Z, Sun X, He S. Red raspberry extract (Rubus idaeus L shrub) intake ameliorates hyperlipidemia in HFD-induced mice through PPAR signaling pathway. Food Chem. Toxicol. 133: 110796 (2019)   DOI
16 Wong TY, Lin SM, Leung LK. The flavone luteolin suppresses SREBP-2 expression and post-translational activation in hepatic cells. PLoS One. 10: e0135637 (2015)   DOI
17 Dikkers A, Tietge UJF. Biliary cholesterol secretion: More than a simple ABC. World J. Gastroenterol. 16: 5936-5945 (2010)
18 Goldstein JL, Brown MS. Regulation of the mevalonate pathway. Nature. 343: 425-430 (1990)   DOI
19 Jia L, Betters JL, Yu L. Niemann-Pick C1-Like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport. Annual Review of Physiology. 73: 239-259 (2011)   DOI
20 Kim B, Park Y, Wegner CJ, Bolling BW, Lee J. Polyphenol-rich black chokeberry (Aronia melanocarpa) extract regulates the expression of genes critical for intestinal cholesterol flux in Caco-2 cells. J Nutr Biochem. 24: 1564-1570 (2013)   DOI
21 Kashani A, Sallam T, Bheemreddy S, Mann DL, Wang Y, Foody JM. Review of side-effect profile of combination ezetimibe and statin therapy in randomized clinical trials. Am. J. Cardiol. 101: 1606-1613 (2008)   DOI
22 Lee D-H, Choi S-S, Kim B-B, Kim S-Y, Kang B-S, Lee S-J, Park H-J. Effect of alcohol-free red wine concentrates on cholesterol homeostasis: An in vitro and in vivo study. Process Biochemistry. 48: 1964-1971 (2013)   DOI
23 Quinones M, Miguel M, Aleixandre A. The polyphenols, naturally occurring compounds with beneficial effects on cardiovascular disease. Nutr. Hosp. 27: 76-89 (2012)
24 Sato R. Sterol metabolism and SREBP activation. Archives of Biochemistry and Biophysics. 501: 177-181 (2010)   DOI
25 Chambers KF, Day PE, Aboufarrag HT, Kroon PA. Polyphenol effects on cholesterol metabolism via bile acid biosynthesis, CYP7A1: a review. Nutrients. 11 (2019)
26 Chiang JY. Regulation of bile acid synthesis: pathways, nuclear receptors, and mechanisms. J. Hepatol. 40: 539-551 (2004)   DOI
27 Abel T, Feher J. Statin therapy and muscle disorders. Orv Hetil. 150: 261-263 (2009)   DOI
28 Brown MS, Goldstein JL. Sterol regulatory element binding proteins (SREBPs): controllers of lipid synthesis and cellular uptake. Nutr Rev. 56: S1-3; discussion S54-75 (1998)
29 Chung S, Gebre AK, Seo J, Shelness GS, Parks JS. A novel role for ABCA1-generated large pre-beta migrating nascent HDL in the regulation of hepatic VLDL triglyceride secretion. J. Lipid Res. 51: 729-742 (2010)
30 Groen AK, Bloks VW, Verkade H, Kuipers F. Cross-talk between liver and intestine in control of cholesterol and energy homeostasis. Mol. Aspects Med. 37:77-88 (2014)   DOI