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http://dx.doi.org/10.9721/KJFST.2021.53.6.775

Characteristics of butyric acid bacterium, Clostridium butyricum DIMO 52, isolated from feces of Korean breastfeeding infants  

Mo, SangJoon (Medical Laser Research Center, Dankook University)
Publication Information
Korean Journal of Food Science and Technology / v.53, no.6, 2021 , pp. 775-784 More about this Journal
Abstract
After isolating the DIMO 52 strain with a large inhibition zone diameter for Clostridium perfringens and maximum butyric acid production from the fecal sample of a breastfeeding infant, it was identified as Clostidium butyricum. The maximum growth of the DIMO 52 strain was reached 24 h after inoculation, and the maximum butyric acid concentration was approximately 34.73±4.27 mM. The DIMO 52 strain survived approximately 67.5% of the initial inoculum at pH 2.0, and approximately 64.9% survived in RCM broth supplemented with 0.3% (w/v) oxgall. In addition, DIMO 52 showed antibacterial activity against Escherichia coli KCTC 2441 and Salmonella Typhimurium KCTC 1925. In LPS-stimulated RAW264.7 cells, 1×103 CFU/mL viable cells of the DIMO 52 strain also exhibited significant NO (nitric oxide) production inhibitory activity (33%, p<0.01). This result suggests that C. butyricum DIMO 52 has anti-inflammatory activity related to NO radical-scavenging activity. In conclusion, C. butyricum DIMO 52 isolated in this study has the potential to be used as a probiotic.
Keywords
Anaerobic bacteria; butyric acid; Clostridium butyricum; gut microbiota; probiotics;
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