Browse > Article
http://dx.doi.org/10.9721/KJFST.2020.52.6.580

Validation of a trienzyme-Lactobacillus casei method for folate analysis in fishery resources consumed in the Korean diet  

Jeong, Bomi (Department of Food Science and Technology, Sunchon National University)
Nam, Ki-Ho (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Kim, Yeon-Kye (Food Safety and Processing Research Division, National Institute of Fisheries Science)
Chun, Jiyeon (Department of Food Science and Technology, Sunchon National University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.6, 2020 , pp. 580-586 More about this Journal
Abstract
Fishery resources have been widely consumed as protein- and vitamin-rich food sources in the Korean diet. However, information regarding their vitamin levels is extremely limited. In this study, trienzyme-Lactobacillus casei method was validated and used to determine the folate contents in fishery foods. The trienzyme-L. casei method for folate analysis showed excellent accuracy (85.2 to 95.3% recovery) and precision (repeatability 1.4% RSD and reproducibility 2.4% RSD). Folate contents of 20 fish foods (4 fish, 3 crustaceans, 3 sea algae, 3 cephalopods, 4 shellfish, and 3 others) ranged from 1.75 to 97.98 ㎍/100 g. Furthermore, we found that the folate content in seaweed fusiforme was the highest, followed by gulfweed (69.73 ㎍/100 g). Folate analysis using the trienzyme-L. casei method was determined excellent based on the z-score of -0.3 in the Food Analysis Performance Assessment Scheme test. Analytical and method validation data generated in this study could be used to update the national food composition table on vitamin B9 in Korean fishery resources.
Keywords
fishery resources; folate; validation; database; vitamin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kucukkolbasi S, Bilber O, Ayyildiz HF, Kara H. Simultaneous and accurate determination of water-and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method. Quim. Nova 36: 1044-1051 (2013)   DOI
2 Ministry of Education, Culture, Sports, Science and Technology (MEXT). Standard Tables of Food Composition in Japan. 7th ed. Tokyo, Japan. pp. 120-151 (2015)
3 Ministry of Health and Welfare, The Korean Nutrition Society. Dietary reference intakes for Koreans 2015. Sejong. pp. 489-493 (2015)
4 Park SJ, Park SH, Chung HJ, Lee JS, Hyun TS, Chun JY. Effects of different cooking methods on folate retention in selected mushrooms. Korean J. Food Preserv. 24: 1103-1112 (2017)   DOI
5 Ruggeri S, Vahteristo LT, Aguzzi A, Finglas P, Carnovale E. Determination of folate vitamers in food and in Italian reference diet by high-performance liquid chromatography. J. Chromatogr. A. 855: 237-245 (1999)   DOI
6 Rural Development Administration (RDA). National Standard Nutrient Database. 9th ed. Jeonju, Korea. pp. 370-373 (2017)
7 Tamura T. Microbiological assay of folates. In Folic Acid Metabolism in Health and Disease. Picciano MF, Stokstad ELR, Gregory JF, eds. John Wiley & Sons Press, New York, NY, USA. pp. 121-137 (1990)
8 Woortman DV, Fuchs T, Striegel L, Fuchs M, Weber N, Brck TB, Rychlik M. Microalgae a superior source of folates: Quantification of folates in halophile microalgae by stable isotope dilution assay. Front. Bioeng. Biotechnol. 7: 481 (2020)   DOI
9 Yon MY, Hyun TS. Additional data for the folate database for foods common in Korea. J. Nutr. Health. 38: 586-604 (2005)
10 Youn HS. New nutritional concepts of vitamins and minerals. Korean J. Pediatr. 12: 1295-1309 (2005)
11 Chun, J, Martin JA, Chen L, Lee J, Ye L, Eitenmiller RR. A differential assay of folic acid and total folate in foods containing enriched cereal-grain products to calculate ㎍ dietary folate equivalents (㎍ DFE). J. Food Compos. Anal. 19: 182-187 (2006)   DOI
12 Arcot J, Shrestha A. Folate: methods of analysis. Trends Food Sci. Tech. 16: 253-266 (2005)   DOI
13 Bailey LB. Folate in health and disease. 2nd ed. CRC Press. Boca Raton, FL, USA. pp. 2-19 (2010)
14 Ahn HS, Kim JS, Lee GJ, Kim YT. Serum folate levels of maternalumbilical cord blood and pregnancy outcomes. Korean J. Food Nutr. 33: 840-847 (2000)
15 Alaburda J, de Almeida AP, Shundo L, Ruvieri V, Sabino M. Determination of folic acid in fortified wheat flours. J. Food Compost. Anal. 21: 336-342 (2008)   DOI
16 Chen L, Eitenmiller RR. Single laboratory method performance evaluation for the analysis of total food folate by trienzyme extraction and microplate assay. J. Food Sci. 72: 243-247 (2007)
17 De Quirs ARB, De Ron CC, Lopez-Hernandez J, Lage-Yusty MA. Determination of folates in seaweeds by high-performance liquid chromatography. J. Chromatogr. A. 1032: 135-139 (2004)   DOI
18 Kariluoto S, Vahteristo L, Finglas PM, van den Berg H, Carnovale E, Jagerstad M. Intercomparison of current methods for folate determination in food. Proceeding of Fourth International Food Data Conference, Bratislava, Slovakia. pp. 40-41 (2001)
19 Kris-Etherton PM, Harris WS, Appel LJ. Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. Circulation 106: 2747-2757 (2002)   DOI
20 AOAC. AOAC guidelines for single laboratory validation of chemical methods for dietary supplements and baotanicals. Association of Official Analytical Chemists, Gaithersburg, MD, USA. pp. 2,12-13,22,25,26 (2002)