Browse > Article
http://dx.doi.org/10.9721/KJFST.2020.52.2.191

Anti-diabetic effect of mulberry leaf extract fermented with Lactobacillus plantarum  

Choi, Jisu (Department of Food Science and Biotechnology, Gachon University)
Lee, Sulhee (Department of Food Science and Biotechnology, Gachon University)
Park, Young-Seo (Department of Food Science and Biotechnology, Gachon University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.2, 2020 , pp. 191-199 More about this Journal
Abstract
The purpose of this study was to isolate novel lactic acid bacteria to ferment mulberry leaf extract (MLE) and to investigate its anti-diabetic effect. Lactobacillus plantarum SG-053 isolated from gatkimchi was selected to ferment MLE because it exhibited high α-glucosidase inhibitory activity (96.8%) and enhanced the content of 1-deoxynojirimycin (DNJ), an anti-diabetic substance, in fermented MLE up-to 2.2 times. MLE fermented with L. plantarum SG-053 (FMLE) showed growth promoting activity against L6 myotubes and increased the gene expressions of IRS-1, PI3K p85α, and GLUT-4 up-to 1.4, 2.2, and 1.4 times, respectively, and 2-deoxyglucose uptake up-to 40.7%. In rat skeletal muscle tissue, the expressions of PI3K p85α and GLUT-4 increased by 6.4 and 2.1 times, respectively. These results suggest that L. plantarum SG-053 could enhance the DNJ content of MLE by fermentation and that FMLE is effective in ameliorating insulin resistance via activation of the insulin signaling pathway.
Keywords
Mulberry leaf; 1-deoxynojirimycin; anti-diabetic effect; Lactobacillus plantarum;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lee WC, Kim AJ, Kim SY. The study on the functional materials and effects of mulberry leaf. Food Sci. Ind. 36: 2-14 (2003)
2 Lee HJ, Lee H, Choi YI, Lee JJ. Effect of lactic acid bacteria-fermented mulberry leaf extract on the improvement of intestinal function in rats. Korean J. Food Sci. An. 37: 561-570 (2017)   DOI
3 Lee S, Lynn EG, Kim JA, Quon MJ. Protein kinase C-ae phosphorylates insulin receptor substrate-1, -3 and -4 but not -2: isoform specific determinants of specificity in insulin signaling. Endocrinology 149: 2451-2458 (2008)   DOI
4 Lee S, Park YS. Oligosaccharide production by Leuconostoc lactis CCK940 which has glucansucrase activity. Food Eng. Prog. 21: 383-390 (2017)   DOI
5 Lee D, Yu JS, Lee SR, Hwang GS, Kang KS, Park JG, Kim HY, Kim KH, Yamabe N. Beneficial effects of bioactive compounds in mulberry fruits against cisplatin-induced nephrotoxicity. Int. J. Mol. Sci. 19: E1117 (2018)   DOI
6 Li YG, Ji DF, Zhong S, Lin TB, Lv ZQ, Hu GY, Wang X. 1-Deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci. Rep. 3: 1377 (2013)   DOI
7 Li X, Wang N, Yin B, Fang D, Zhao Z, Zhang H, Wang G, Chen W. Lactobacillus plantarum X1 with ${\alpha}$-glucosidase inhibitory activity ameliorates type 2 diabetes in mice. RSC Adv. 6: 63536-63547 (2016)   DOI
8 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-{\Delta}{\Delta}CT}$ method. Methods 25: 402-408 (2001)   DOI
9 Mussig K, Staiger H, Fiedler H, Moeschel K, Beck A, Kellerer M, Haring HU. Shp2 is required for protein kinase C-dependent phosphorylation of serine 307 in insulin receptor substrate-1. J. Biol. Chem. 280: 32693-32699 (2005)   DOI
10 Park JM, Bong HY, Jeong HI, Kim YK, Kim JY, Kwon O. Postprandial hypoglycemic effect of mulberry leaf in Goto-Kakizaki rats and counterpart Wistar rats. Nutr. Res. Pract. 3: 272-278 (2009)   DOI
11 Ramchandran L, Shah NP. Effect of exopolysaccharides and inulin on the proteolytic, angiotensin-I-converting enzyme- and ${\alpha}$-glucosidase-inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage. J. Dairy Sci. 92: 895-906 (2009)   DOI
12 Ryu IH, Kwon TO. Enhancement of piperidine alkaloid contents by lactic acid fermentation of mulberry leaves (Morus alba L.). Korean J. Med. Crop Sci. 20: 472-478 (2012)   DOI
13 Saito K, Lee S, Shiuchi T, Toda C, Kamijo M, Inagaki-Ohara K, Okamoto S, Minokoshi Y. An enzymatic photometric assay for 2-deoxyglucose uptake in insulin-responsive tissues and 3T3-L1 adipocytes. Anal. Biochem. 412: 9-17 (2011)   DOI
14 Takasu S, Parida IS, Onose S, Ito J, Ikeda R, Yamagishi K, Higuchi O, Tanaka F, Kimura T, Miyazawa T, Nakagawa K. Evaluation of the anti-hyperglycemic effect and safety of microorganism 1-deoxynojiriycin. PLoS One 13: e0199057 (2018)   DOI
15 Zhong Y, Wu S, Chen F, He M, Lin J. Isolation of high ${\gamma}$-aminobutyric acid-producing lactic acid bacteria and fermentation in mulberry leaf powders. Exp. Ther. Med. 18: 147-153 (2019)
16 Cai S, Sun W, Fan Y, Guo X, Xu G, Xu T, Hou Y, Zhao B, Feng X, Liu T. Effect of mulberry leaf (Folium Mori) on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus rats. Pharm. Biol. 54: 2685-2691 (2016)   DOI
17 Asano N, Oseki K, Tomioka E, Kizu H, Matsui K. N-Containing sugars from Morus alba and their glycosidase inhibitory activities. Carbohyd. Res. 259: 243-255 (1994)   DOI
18 Bae UJ, Jung ES, Jung SJ, Chae SW, Park BH. Mulberry leaf extract displays antidiabetic activity in db/db mice via Akt and AMPactivated protein kinase phosphorylation. Food Nutr. Res. 62: 1473 (2018)
19 Bartoli E, Fra GP, Carnevale Schianca GP. The oral glucose tolerance test (OGTT) revisited. Eur. J. Intern. Med. 22: 8-12 (2011)   DOI
20 Bruning JC, Michael MD, Winnay JN, Hayashi T, Horsch D, Accili D, Goodyear LJ, Kahn CR. A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Mol. Cell. 2: 559-569 (1998)   DOI
21 Caplice E, Fitzgerald GF. Food fermentations: Role of microorganisms in food production and preservation. Int. J. Food. Microbiol. 50: 131-149 (1999)   DOI
22 Carnevali O, Vivo L, Sulpizio R, Gioacchini G, Olivotto I, Silvi S, Cresci A. Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression. Aquaculture 258: 430-438 (2006)   DOI
23 Ceriello A. Postprandial hyperglycemia and diabetes complications: is it time to treat? Diabetes 54: 1-7 (2005)   DOI
24 Choi SW, Lee YJ, Ha SB, Jeon YH, Lee DH. Evaluation of biological activity and analysis of functional constituents from different parts of mulberry (Morus alba L.) tree. J. Korean Soc. Food Sci. Nutr. 44: 823-831 (2015)   DOI
25 Hitendra J, Narotham Prasad BD, Gurumurthy H, Suvarna VC. Role of lactic acid bacteria (LAB) in food preservation. Int. J. Curr. Microbiol. Appl. Sci. 5: 255-257 (2016)   DOI
26 Cubas-Cano E, Gonzales-Fernandez C, Tomas-Pejo E. Evolutionary engineering of Lactobacillus pentosus improves lactic acid productivity from xylose-rich media at low pH. Bioresource Technol. 288: 121540 (2019)   DOI
27 Czech MP, Corvera S. Signaling mechanisms that regulate glucose transport. Biol. Chem. 274: 1865-1868 (1999)   DOI
28 Gao K, Zheng C, Wang T, Zhao H, Wang J, Wang Z, Zhai X, Jia Z, Chen J, Zhoi Y, Wang W. 1-Deoxynojirimycin: occurrence, extraction, chemistry, oral pharmacokinetics, biological activities and in silico target fishing. Molecules 21: E1600 (2016)   DOI
29 Gual P, Le Marchand-Brustel Y, Tanti JF. Positive and negative regulation of insulin signaling through IRS-1 phosphorylation. Biochimie. 87: 99-109 (2005)   DOI
30 Harano Y, Sakamoto A, Izumi K, Shimizu Y, Hoshi M, Shichiri M, Shigeta Y, Ohgaku S, Abe H. Usefulness of maltose for testing glucose tolerance. Am. J. Clin. Nutr. 30: 924-931 (1977)   DOI
31 Holzapfel WH, Schillinger U. Introduction to pre- and probiotics. Food Res. Int. 35: 109-116 (2002)   DOI
32 Honda K, Moto M, Uchida N, He F, Hashizume N. Anti-diabetic effects of lactic acid bacteria in normal and type 2 diabetic mice. J. Clin. Biochem. Nutr. 51: 96-101 (2012)   DOI
33 Huang CH, Chen MF, Chung HH, Cheng JT. Antihyperglycemic effect of syringaldehyde in streptozotocin-induced diabetic rats. J. Nat. Prod. 75: 1465-1468 (2012)   DOI
34 Huang X, Liu G, Gou J, Su Z. The PI3K/AKT pathway in obesity and type 2 diabetes. Int. J. Biol. Sci. 14: 1483-1496 (2018)   DOI
35 Kang DH, Khil LY, Park KS, Lee BH, Moon CK. Effects of cadmium on glucose transport in L6 myocytes. J. Environ. Toxicol. 20: 75-85 (2005)
36 Huang DW, Shen SC, Wu JSB. Effects of caffeic acid and cinnamic acid on glucose uptake in insulin-resistant mouse hepatocytes. J. Agr. Food Chem. 57: 7687-7692 (2009)   DOI
37 Jeon HJ, Kim SH, Lee BY. Oral glucose and maltose tolerance test and inhibition effect of a-glucosidase of Ecklonia cava extract (seapolynol) and dieckol. J. Korean Soc. Food Sci. Nutr. 47: 347-351 (2018)   DOI
38 Jeong JH, Lee NK, Cho SH, Jeong DY, Jeong YS. Enhancement of 1-deoxynojirimycin content and a-glucosidase inhibitory activity in mulberry leaf using various fermenting microorganisms isolated from Korean traditional fermented food. Biotechnol. Bioproc. E. 19: 1114-1118 (2014)   DOI
39 Jung SH, Han JH, Park HS, Lee DH, Kim SJ, Cho HS, Kang JS, Myung CS. Effects of unaltered and bioconverted mulberry leaf extracts on cellular glucose uptake and antidiabetic action in animals. BMC Complem. Altern. M. 19: 55 (2019)   DOI
40 Kan J, Velliquette RA, Grann K, Burns CR, Scholten J, Tian F, Zhang Q, Gui M. A novel botanical formula prevents diabetes by improving insulin resistance. BMC Complem. Altern. M. 17: 352 (2017)   DOI
41 Kim GN, Kwon YI, Jang HD. Mulberry leaf extract reduces postprandial hyperglycemia with few side effects by inhibiting a-glucosidase in normal rats. J. Med. Food. 14: 712-717 (2011)   DOI
42 Kojima Y, Kimura T, Nakagawa K, Asai A, Hasumi K, Oikawa S, Miyazawa T. Effects of mulberry leaf extract rich in 1-deoxynojirimycin on blood lipid profiles in humans. J. Clin. Biochem. Nutr. 47: 155-161 (2010)   DOI
43 Lee WJ, Choi SW. Quantitative changes of polyphenolic compounds in mulberry (Morus alba L.) leaves in relation to varieties, harvest period, and heat processing. Prev. Nutr. Food. Sci. 17: 280-285 (2012)   DOI